Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC)
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Generation of Human Brain Organoids for Mitochondrial Disease Modeling
Stephanie Le 1, Laura Petersilie 2, Gizem Inak 1,4, Carmen Menacho-Pando 1, Karl W. Kafitz 2, Agnieszka Rybak-Wolf 3, Nikolaus Rajewsky 3, Christine R. Rose 2, Alessandro Prigione 1,5
1Department of General Pediatrics, Neonatology and Pediatric Cardiology, Duesseldorf University Hospital, Medical Faculty, Heinrich Heine University, 2Institute of Neurobiology, Heinrich Heine University, 3Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrueck Center for Molecular Medicine (MDC), 4Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, 5Max Delbrueck Center for Molecular Medicine (MDC)
We describe a detailed protocol for the generation of human induced pluripotent stem cell-derived brain organoids and their use in modeling mitochondrial diseases.
Biology
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
Janna Leiz *1,2, Christian Hinze *1,2, Anastasiya Boltengagen 3, Caroline Braeuning 4, Christine Kocks 3, Nikolaus Rajewsky 3, Kai M. Schmidt-Ott 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.
