Accedi

Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC)

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Generation of Human Brain Organoids for Mitochondrial Disease Modeling

Stephanie Le¹, Laura Petersilie², Gizem Inak^1,4, Carmen Menacho-Pando¹, Karl W. Kafitz², Agnieszka Rybak-Wolf³, Nikolaus Rajewsky³, Christine R. Rose², Alessandro Prigione^1,5

¹Department of General Pediatrics, Neonatology and Pediatric Cardiology, Duesseldorf University Hospital, Medical Faculty, Heinrich Heine University, ²Institute of Neurobiology, Heinrich Heine University, ³Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrueck Center for Molecular Medicine (MDC), ⁴Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, ⁵Max Delbrueck Center for Molecular Medicine (MDC)

We describe a detailed protocol for the generation of human induced pluripotent stem cell-derived brain organoids and their use in modeling mitochondrial diseases.

Biology

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Janna Leiz^*1,2, Christian Hinze^*1,2, Anastasiya Boltengagen³, Caroline Braeuning⁴, Christine Kocks³, Nikolaus Rajewsky³, Kai M. Schmidt-Ott^1,2

¹Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, ²Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ³Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ⁴Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)

Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.