Renaissance School of Medicine at Stony Brook University

3 ARTICLES PUBLISHED IN JoVE

Biology

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Agnieszka B. Bialkowska^*1, Amr M. Ghaleb^*1, Mandayam O. Nandan¹, Vincent W. Yang^1,2

¹Department of Medicine, Stony Brook University School of Medicine, ²Department of Physiology & Biophysics, Stony Brook University School of Medicine

Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.

Biology

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Emilia J. Orzechowska-Licari¹, Joseph F. LaComb¹, Michael Giarrizzo¹, Vincent W. Yang^1,2, Agnieszka B. Bialkowska¹

¹Department of Medicine, Renaissance School of Medicine at Stony Brook University, ²Department of Physiology and Biophysics, Renaissance School of Medicine at Stony Brook University

The gastrointestinal tract is one of the most sensitive organs to injury upon radiotherapeutic cancer treatments. It is simultaneously an organ system with one of the highest regenerative capacities following such insults. The presented protocol describes an efficient method to study the regenerative capacity of the intestinal epithelium.

Neuroscience

Functional Magnetic Resonance Imaging (fMRI) of the Visual Cortex with Wide-View Retinotopic Stimulation

Andre Galenchik-Chan^*1, Diane Chernoff^*1, Wei Zhou², Timothy Q. Duong³, Eric R. Muir⁴

¹Renaissance School of Medicine at Stony Brook University, ²Department of Radiology, University of Colorado School of Medicine, ³Department of Radiology, Montefiore-Einstein, ⁴Department of Radiology, School of Medicine, Stony Brook University

We have developed techniques for mapping the visual cortex function utilizing more of the visual field than is commonly used. This approach has the potential to enhance the evaluation of vision disorders and eye diseases.