Sichuan University and Collaborative Innovation Center

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Huayun Yan¹, Yingfang Ma¹, Xinyue Zhou², Yushuang He³, Yang Liu², Carlos Caulin⁴, Leiming Wang⁵, Heng Xu^1,6, Han Luo^2,6

¹State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center, ²Division of Thyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-Related Molecular Network, Department of General Surgery, West China Hospital, Sichuan University, ³Department of Ultrasound, West China Hospital of Sichuan University, Sichuan University, ⁴Department of Otolaryngology-Head & Neck Surgery and University of Arizona Cancer Center, University of Arizona, ⁵Center for Translational Medicine, Shenzhen Bay Laboratory, ⁶Division of Laboratory Medicine/Research Centre of Clinical Laboratory Medicine, West China Hospital, Sichuan University

Here, we present a standard pipeline to obtain murine ATC tumors by spontaneous genetically engineered mouse models. Further, we present tumor dynamics and pathological information about the primary and metastasized lesions. This model will help researchers to understand tumorigenesis and facilitate drug discoveries.

Immunology and Infection

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Yuanfan Qian^1,2, Linye He^1,2, Anping Su^1,2, Yiguo Hu³, Jingqiang Zhu¹

¹Thyroid and Parathyroid Surgery Center, West China Hospital of Sichuan University, ²The Laboratory of Thyroid and Parathyroid Disease, West China Hospital of Sichuan University, ³State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center

Several types of animal models of Hashimoto's thyroiditis have been established, as has spontaneous autoimmune thyroiditis in the NOD mouse. H-2h4 mice are a simple and reliable model for HT induction. This article describes this approach and evaluates the pathological process for a better understanding of the SAT murine model.

Cancer Research

High-Resolution Ultrasonography for the Analysis of Orthotopic ATC Tumors in a Genetically Engineered Mouse Model

Yushuang He¹, Yan Luo¹, Zhihui Li², Han Luo², Huayun Yan³, Yingfang Ma³, Xinyue Zhou²

¹Department of Ultrasound, West China Hospital, Sichuan University, ²Department of Thyroid and Parathyroid Surgery, Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, ³State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center

The present protocol describes high-frequency ultrasonography for visualizing the entire mouse thyroid gland and monitoring the growth of anaplastic thyroid carcinoma.