Peak Proteins Ltd.

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Ewa Rejnowicz¹, Jack Wright¹, Margaret Veldman-Jones¹, Steven Harborne¹

The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.