Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion ChromatographyEwa Rejnowicz 1, Jack Wright 1, Margaret Veldman-Jones 1, Steven Harborne 1
1Peak Proteins Ltd.
The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.
