小鼠幼稚 CD4⁺ T 细胞体外分化为致病性 Th17 细胞

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2.3K Views

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07:46 min

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October 25th, 2024

DOI :

10.3791/66718-v

October 25th, 2024

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Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng¹^,², Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University

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摘要

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JoVE 212 CD4 T Th17

此视频中的章节

0:00

Introduction

1:38

Isolation and Preparation of Mice Splenic Single‐Cell Suspension for Flow Cytometry Analysis

3:45

Purification and Analysis of Mice Naive CD4⁺ T‐Cells Using Magnetic Bead‐Based Negative Selection

5:57

In Vitro Induction of Pathogenic Th17 Cells into Naive CD4⁺ T‐Cells of Mice

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