In vitro Differenzierung von naiven CD4+ T-Zellen in pathogene Th17-Zellen in der Maus
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3.1K Views
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07:46 min
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October 25th, 2024
DOI :
October 25th, 2024
•Haofei Wang*1, Xu Liu*1, Wei Huang1, Xiaojing Wu1, Ying Tang1, Mingzhu Zheng1,2, Haibo Qiu1, Yingzi Huang1
1Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, 2Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University
Transkript
Kapitel in diesem Video
0:00
Introduction
1:38
Isolation and Preparation of Mice Splenic Single‐Cell Suspension for Flow Cytometry Analysis
3:45
Purification and Analysis of Mice Naive CD4+ T‐Cells Using Magnetic Bead‐Based Negative Selection
5:57
In Vitro Induction of Pathogenic Th17 Cells into Naive CD4+ T‐Cells of Mice
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