In Vitro Differentiation of Naive CD4⁺ T Cells into Pathogenic Th17 Cells in Mouse

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2.3K Views

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07:46 min

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October 25th, 2024

DOI :

10.3791/66718-v

October 25th, 2024

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Haofei Wang*¹, Xu Liu*¹, Wei Huang¹, Xiaojing Wu¹, Ying Tang¹, Mingzhu Zheng¹^,², Haibo Qiu¹, Yingzi Huang¹

¹Jiangsu Provincial Key Laboratory of Critical Care Medicine, Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University, ²Department of Pathogenic Biology and Immunology, Jiangsu Provincial Key Laboratory of Critical Care Medicine, School of Medicine, Southeast University

Transkrypcja

Podsumowanie

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In Vitro Differentiation

Naive CD4 T Cells

Pathogenic Th17 Cells

Th17 Cell Induction

Differentiation Purity

Flow Cytometry Sorting

Magnetic Bead Sorting

Autoimmune Disorders

Multiple Sclerosis

Rheumatoid Arthritis

Acute Respiratory Distress Syndrome

Sepsis

Differentiation Protocols

Splenocyte Isolation

Mouse Model

Rozdziały w tym wideo

0:00

Introduction

1:38

Isolation and Preparation of Mice Splenic Single‐Cell Suspension for Flow Cytometry Analysis

3:45

Purification and Analysis of Mice Naive CD4⁺ T‐Cells Using Magnetic Bead‐Based Negative Selection

5:57

In Vitro Induction of Pathogenic Th17 Cells into Naive CD4⁺ T‐Cells of Mice

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In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

In Vitro Differentiation of Naive CD4⁺ T Cells into Pathogenic Th17 Cells in Mouse