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Collection of Subendothelial Matrix from Retinal Microvascular Endothelial Cell (REC) Cultures for Atomic Force Microscope (AFM) Stiffness Measurement

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02:01 min

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July 12th, 2024

DOI :

10.3791/201965-v

July 12th, 2024

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18 Views

Irene Santiago Tierno¹^,²^,³, Mahesh Agarwal¹^,², Nikolaos Matisioudis²^,⁴, Sathishkumar Chandrakumar¹^,², Kaustabh Ghosh¹^,²^,³

¹Department of Ophthalmology, University of California, Los Angeles, ²Doheny Eye Institute, ³Molecular, Cellular, and Integrative Physiology Interdepartmental PhD Program, University of California, Los Angeles, ⁴Department of Bioengineering, University of Pennsylvania

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Subendothelial Matrix

Retinal Microvascular Endothelial Cells

Atomic Force Microscope

AFM Stiffness Measurement

Glutaraldehyde Cross linking

Decellularization Buffer

Phase Contrast Microscope

RNase Free DNase

Cellular Debris Removal

Nanoscale Matrix Fibers

Fibrous Matrix Aggregates

Fibronectin Matrix

来自系列

Measurement of Stiffness in Mouse Retinal Capillaries and Subendothelial Matrix

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