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1。鼠标膀胱壁注射液小鼠品系，年龄和性别的选择是出于实验需要。我们用8至12周龄小鼠，因为这是一个窗口前的免疫成熟到衰老。作为一般指引，老鼠应该到达至少一个星期前，实验操作，以避免应力引起的混杂因素。 <ol><li>用肥皂和水清洁手术台面。 </li><li> CIDE挥笔或杀菌湿巾擦拭手术台面。 </li><li>前高压灭菌干净的手术器械，手术中使用。 </li><li>此外，热珠灭菌消毒的手术器械之前使用，以及在手术过程中的动物之间。 </li><li>清洁100μL，汉密尔顿注射器和29或30号（1 / 2英寸长）针头反复抽吸和无水酒精注射，第一次使用前，在最后的外科手术结束。 </li><li>清洗和冲洗消毒磷酸盐缓冲生理盐水每只动物之间的汉密尔顿注射器。 </li><li>麻醉小鼠放置在2-5％之间，异氟醚，异氟醚诱导室。 </li><li>一旦达到全身麻醉室和放置在一个温暖的垫仰卧位，请从鼠标的下方，以帮助维持正常体温。 </li><li>实现维护麻醉动物的口鼻部放置在一个喷嘴，含挥发异氟醚（滴定从1-3％，要保持适当的麻醉）。 </li><li>剃须与快船队的腹部皮肤。 </li><li>使用一次性无菌手术悬垂覆盖，以防止在手术过程中粪便污染肛门。 </li><li>使用第二个一次性无菌手术悬垂覆盖手术野（小腹）。 </li><li>准备三件浸泡优碘，纱布，腹部。重复此步骤三次。 </li><li>为解剖显微镜的放大倍率，使较低的中线腹部切口，用剪刀。 </li><li>公开膀胱。 </li><li>如果膀胱已满，部分解压缩温柔在圆顶的下调压力。 </li><li>注入样品溶液（高达50μL），使用29或30号针头和注射器，进入膀胱球与斜面朝上针（壁间注射）壁。 </li><li>推注射器推杆注入膀胱壁的样品溶液。良好的本地化的气泡，是一个成功注射到膀胱壁细胞的迹象。 </li><li>取下注射器，关闭切口，伤口剪辑，让鼠标恢复变暖垫。 </li></ol> 2。代表性的成果： 一个良好的本地化的气泡，不漏液，并保持稳定的规模是一个成功注射到膀胱壁细胞（图1）的指示。组织学分析，可以确认注入膀胱壁细胞的存在。 <img src="/files/ftp_upload/2523/2523fig1.jpg" alt="图1"/> 图1。成功的膀胱壁注射印度墨水使用插图的例子。 <img src="/files/ftp_upload/2523/2523fig2.jpg" alt="图2"/> 图2实验步骤示意图。

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小鼠膀胱壁注射允许为特定的细胞植入到膀胱壁的定义领域。这项技术已广泛应用，为膀胱癌，平滑肌，和干细胞生物学的小鼠模型。根据定义，小鼠膀胱壁注射液有利于引入膀胱癌或干细胞原位位置，所以可以在一个有关生理解剖背景下的研究，他们的生长和分化。事实上，Dinney等。首次描述了这种技术，以研究在1裸鼠人膀胱癌裸鼠移植瘤原位。 虽然一直在膀胱?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>异氟醚（Aerrane） </td><td>巴克斯特</td><td> NDC的10019-773-60 </td></tr><tr><td> 29或30表针</td><td>汉密尔顿</td><td> 7803-06或7803-07 </td></tr><tr><td> 100微升注射器</td><td>汉密尔顿</td><td> 7656-01 </td></tr></tbody></table>

mouse bladder wall injection

小鼠膀胱壁注射原位研究，包括干细胞，平滑肌，和癌症生物学膀胱现象，是一种有用的技术。开始前注射，手术区必须用肥皂和水和消毒液清洗。在使用前和每个动物之间的手术设备必须消毒。每个鼠标放在吸入异氟醚麻醉下（2-5％的诱导，维持1-3％）和膀胱暴露中线腹部切口，用剪刀。如果膀胱满了，这是部分之间的两根手指轻轻挤压解压。 intramurally利益的细胞悬液注入使用29或30号针头和注射器1毫升或更小的圆顶膀胱壁。伤口闭合伤口剪辑，并允许恢复变暖垫鼠标。膀胱壁注射液是一种微妙的显微外科技术，可以掌握与实践。

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Mouse Bladder Wall Injection

小鼠膀胱壁注射液是一种有用的方法原位研究膀胱干细胞和癌症生物学。这种微妙的显微外科方法可以掌握仔细的技术和实践。

鼠标膀胱壁注射液

Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. ...

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23.8K 次观看.  Stanford University School of Medicine. 小鼠膀胱壁注射液是一种有用的方法原位研究膀胱干细胞和癌症生物学。这种微妙的显微外科方法可以掌握仔细的技术和实践。