August 22nd, 2007
•The ability to manipulate human neural stem/precursor cells (hNSPCs) in vitro allows to investigate their utility as cell transplants for therapeutic purposes and to explore human neural development. This protocol presents a method of culturing and passaging hNSPCs in hopes of increasing reproducibility of human stem cell research.
Tags
Related Videos
Passaging HuES Human Embryonic Stem Cell-lines with Trypsin.
Propagation of Human Embryonic Stem (ES) Cells
Transfecting Human Neural Stem Cells with the Amaxa Nucleofector
Counting Human Neural Stem Cells
Immunocytochemistry: Human Neural Stem Cells
Derivation of Human Embryonic Stem Cells by Immunosurgery
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
Chromatin Immunoprecipitation from Human Embryonic Stem Cells
Culture and Maintenance of Human Embryonic Stem Cells
Freezing and Thawing Human Embryonic Stem Cells