July 7th, 2011
•The endoplasmic reticulum plays a key role in protein biogenesis and in calcium homeostasis. We have established an experimental system that allows us to address the role of Ca2+ leak channels and to characterize their putative regulatory mechanisms. This system involves siRNA mediated gene silencing and live cell Ca2+ imaging.
Tags
Related Videos
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+