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Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

17.9K Views

13:40 min

July 7th, 2011

July 7th, 2011

17,943 Views

0:05

Title

2:16

Preparation of Stock Solutions

3:03

Gene Silencing in HeLa Cells

5:39

Complementation of HeLa Cells

7:07

Live Cell Calcium Imaging

9:58

Representative Calcium Imaging Results

12:58

Conclusion

The endoplasmic reticulum plays a key role in protein biogenesis and in calcium homeostasis. We have established an experimental system that allows us to address the role of Ca2+ leak channels and to characterize their putative regulatory mechanisms. This system involves siRNA mediated gene silencing and live cell Ca2+ imaging.

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