A.奥兹坎感激地承认了国家科学基金会职业奖，羚羊青年研究者奖2009年和办公室的主任，国立卫生研究院国立卫生研究院主任的新的创新奖DP2OD006427的支持。作者也承认，比尔和梅林达盖茨基金会，沃达丰美洲基金会，和NSF BISH的程序（＃0754880和0930501奖）的支持。

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没有利益冲突的声明。

我们展示了一个芯片上的荧光显微镜平台，可以实现例如，&lt;4μm的空间分辨率比例如，&gt; 0.6-8厘米2没有使用任何镜头，机械扫描或薄膜干涉滤波器领域的视图。在这种技术中，使用了光纤面板或锥度，从对象的荧光收集，然后传递到如的CCD光电传感器阵列与二维阵列的光纤电缆/ CMOS芯片。这些收购的lensfree图像，然后迅速处理，产生超过4μm的决议&gt; 0.6-8厘米2，使用压缩采样/?...

<table border="1"><tbody><tr><th>材料名称</th><th>公司</th><th>目录编号</th></tr><tr><td>电荷耦合器件（CCD） </td><td>柯达</td><td> KAF - 8300 </td></tr><tr><td>电荷耦合器件（CCD） </td><td>柯达</td><td> KAF - 11002 </td></tr><tr><td>电荷耦合器件（CCD） </td><td>柯达</td><td> KAF - 39000 </td></tr><tr><td>互补金属氧化物半导体（CMOS） </td><td>美光科技公司</td><td> MT9T031C12STCD </td></tr><tr><td>高功率LED光源</td><td> Thorlabs </td><td> M455L2 - C2 </td></tr><tr><td>高功率LED驱动器</td><td> Thorlabs </td><td> LEDD1B </td></tr><tr><td>光纤耦合LED光源</td><td> Mightex </td><td> FCS - 0625 - 000 </td></tr><tr><td>真空笔</td><td>埃德蒙光学</td><td> NT57 - 636 </td></tr><tr><td> 2，4，10微米Fluospheres </td><td> Invitrogen公司</td><td> F - 8826，F - 8859，F - 8836 </td></tr><tr><td> RBS裂解液1X </td><td> eBioscience公司</td><td> 00-4333 </td></tr><tr><td>祥发16标签试剂</td><td> Invitrogen公司</td><td> S7578 </td></tr><tr><td>光纤面板</td><td>埃德蒙光学</td><td> NT55 - 142 </td></tr><tr><td>光纤光锥</td><td>埃德蒙光学</td><td> NT55 - 134 </td></tr><tr><td>棱镜</td><td>埃德蒙光学</td><td> NT47 - 626，NT45 - 403 </td></tr><tr><td>过滤器</td><td>埃德蒙光学</td><td> NT39 - 417 </td></tr><tr><td>硅橡胶弹性体</td><td>道康宁</td><td> Slygard 184 </td></tr></tbody></table>

lensless fluorescent microscopy on a chip

一般无透镜成像芯片，旨在更简单和更紧凑的设计，以取代笨重的镜头，尤其是对高通量筛选应用光学显微镜，。这个新兴的技术平台，以消除潜在需要的笨重和/或昂贵的光学元件，通过新颖的理论和数字重建算法的帮助。按照同样的思路，在这里我们展示了一个芯片上的荧光显微镜模式，可以实现超过超宽领域的视角（FOV）的4μm的空间分辨率&gt; 0.6-8厘米2例如，没有使用任何镜头，机械扫描或基于薄膜干涉滤光片。在这种技术中，荧光激发是通过由非相干源照明棱镜或半球形玻璃界面。与整个物体的体积相互作用后，这激发光全内反射（TIR）的过程，是发生在样品的微流体芯片的底部被拒绝。从激发对象的荧光是由光纤面板或锥度然后收集和交付作为一个电荷耦合器件（CCD）光电传感器阵列。通过使用压缩采样的解码算法，收购lensfree原料样品的荧光图像，可以迅速处理产量，例如，0.6-8厘米2 &lt;4μm的分辨率超过了视野。此外，垂直堆叠，如分离的微通道，50-100微米，也可以成功地使用相同的lensfree片上显微镜平台，这进一步增加了这种方式的整体吞吐量成像。这个紧凑的芯片上的荧光成像平台，它背后的快速压缩解码器，可用于高通量流式细胞仪，罕见的细胞研究和基因芯片分析是相当宝贵的。

Watch this Scientific Journal Video about Lensless Fluorescent Microscopy on a Chip at JoVE.com

Lensless Fluorescent Microscopy on a Chip

一个无透镜片上荧光显微镜平台，证明可以超过，如超宽现场，查看荧光图像对象，&gt; 0.6-8平方厘米&lt;4μm的使用压缩采样的解码算法的决议。这种紧凑型和宽视场荧光片上的成像方式，可用于高通量流式细胞仪，罕见的细胞研究和基因芯片分析的价值。

片上的无透镜荧光显微镜

On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for ...

lensless-fluorescent-microscopy-on-a-chip

Research

JoVE Journal

Bioengineering

17.5K 次观看.  University of California, Los Angeles. 一个无透镜片上荧光显微镜平台，证明可以超过，如超宽现场，查看荧光图像对象，> 0.6-8平方厘米