A. Ozcan gratefully acknowledges the support of NSF CAREER Award, the ONR Young Investigator Award 2009 and the NIH Director&#x2019;s New Innovator Award DP2OD006427 from the Office of The Director, NIH. The authors also acknowledge the support of the Bill &amp; Melinda Gates Foundation, Vodafone Americas Foundation, and NSF BISH program (under Awards # 0754880 and 0930501).

<ol>
	<li>Coskun, A. F., Su, T., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wide+field-of-view+lens-free+fluorescent+imaging+on+a+chip.">Wide field-of-view lens-free fluorescent imaging on a chip.</a> Lab Chip. 10, 824-824 (2010).</li><li>Coskun, A. F., Sencan, I., Su, T., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lensless+wide-field+fluorescent+imaging+on+a+chip+using+compressive+decoding+of+sparse+objects.">Lensless wide-field fluorescent imaging on a chip using compressive decoding of sparse objects.</a> Opt. Express. 18, 10510-10523 (2010).</li><li>Coskun, A. F., Sencan, I., Su, T., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lensfree+Fluorescent+On-Chip+Imaging+of+Transgenic+Caenorhabditis+elegans+Over+an+Ultra-Wide+Field-of-View.">Lensfree Fluorescent On-Chip Imaging of Transgenic Caenorhabditis elegans Over an Ultra-Wide Field-of-View.</a> PLoS ONE. 6, e15955-e15955 (2011).</li><li>Coskun, A. F., Sencan, I., Su, T., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wide-field+lensless+fluorescent+microscopy+using+a+tapered+fiber-optic+faceplate+on+a+chip.">Wide-field lensless fluorescent microscopy using a tapered fiber-optic faceplate on a chip.</a> Analyst. , (2011).</li><li>Seo, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-Throughput+Lens-Free+Blood+Analysis+on+a+Chip.">High-Throughput Lens-Free Blood Analysis on a Chip.</a> Analytical Chemistry. 82, 4621-4627 (2010).</li><li>Mudanyali, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Compact,+light-weight+and+cost-effective+microscope+based+on+lensless+incoherent+holography+for+telemedicine+applications.">Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications.</a> Lab Chip. 10, 1417-1417 (2010).</li><li>Tseng, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lensfree+microscopy+on+a+cellphone.">Lensfree microscopy on a cellphone.</a> Lab Chip. 10, 1787-1787 (2010).</li><li>Lucy, L. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+iterative+technique+for+the+rectification+of+observed+distributions.">An iterative technique for the rectification of observed distributions.</a> The Astronomical Journal. 79, 745-745 (1974).</li><li>Richardson, W. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bayesian-Based+Iterative+Method+of+Image+Restoration.">Bayesian-Based Iterative Method of Image Restoration.</a> J. Opt. Soc. Am. 62, 55-59 (1972).</li><li>Biggs, D. S. C., Andrews, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acceleration+of+iterative+image+restoration+algorithms.">Acceleration of iterative image restoration algorithms.</a> Appl. Opt. 36, 1766-1775 (1997).</li><li>Candes, E., Wakin, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Introduction+To+Compressive+Sampling.">An Introduction To Compressive Sampling.</a> Signal Processing Magazine, IEEE. 25, 21-30 (2008).</li><li>Kim, S., Koh, K., Lustig, M., Boyd, S., Gorinevsky, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Interior-Point+Method+for+Large-Scale+L1-Regularized+Least+Squares.">An Interior-Point Method for Large-Scale L1-Regularized Least Squares.</a> Selected Topics in Signal Processing, IEEE. 1, 606-617 (2007).</li><li>Candes, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+restricted+isometry+property+and+its+implications+for+compressed+sensing.">The restricted isometry property and its implications for compressed sensing.</a> Comptes Rendus Mathematique. 346, 589-592 (2008).</li><li>Baraniuk, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Compressive+Sensing+[Lecture+Notes].">Compressive Sensing [Lecture Notes].</a> Signal Processing Magazine, IEEE. 24, 118-121 (2007).</li><li>Romberg, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+via+Compressive+Sampling.">Imaging via Compressive Sampling.</a> Signal Processing Magazine, IEEE. 25, 14-20 (2008).</li></ol>

No conflicts of interest declared.

We demonstrated an on-chip fluorescent microscopy platform that can achieve e.g., &lt;4&mu;m spatial resolution over e.g., &gt;0.6-8 cm2 field-of-view without the use of any lenses, mechanical-scanning or thin-film interference-filters. In this technique, with the use of a fiber-optic faceplate or a taper, the fluorescent emission from the objects is collected with a 2D-array of fiber-optic cables before being delivered to an opto-electronic sensor-array such as a CCD/CMOS chip. These acquired lensfree images ...

<table border="1">
 <tbody>
 <tr>
 <th>Material Name</th>
 <th>Company</th>
 <th>Catalogue number</th>
 </tr>
 <tr>
 <td>Charge-coupled device(CCD) </td>
 <td>KODAK</td>
 <td>KAF-8300</td>
 </tr>
 <tr>
 <td>Charge-coupled device(CCD)</td>
 <td>KODAK</td>
 <td>KAF-11002</td>
 </tr>
 <tr>
 <td>Charge-coupled device(CCD)</td>
 <td>KODAK</td>
 <td>KAF-39000</td>
 </tr>
 <tr>
 <td>Complementary Metal-Oxide-Semiconductor (CMOS)</td>
 <td>Micron</td>
 <td>MT9T031C12STCD</td>
 </tr>
 <tr>
 <td>High power LED light source</td>
 <td>Thorlabs </td>
 <td>M455L2-C2</td>
 </tr>
 <tr>
 <td>High power LED driver</td>
 <td>Thorlabs</td>
 <td>LEDD1B</td>
 </tr>
 <tr>
 <td>Fiber coupled LED light source</td>
 <td>Mightex</td>
 <td>FCS-0625-000</td>
 </tr>
 <tr>
 <td>Vacuum Pen</td>
 <td>Edmund Optics</td>
 <td>NT57-636</td>
 </tr>
 <tr>
 <td>2, 4, 10 &mu;m Fluospheres</td>
 <td>Invitrogen</td>
 <td>F-8826, F-8859, F-8836</td>
 </tr>
 <tr>
 <td>RBS lysis buffer 1X </td>
 <td>eBioscience</td>
 <td>00-4333</td>
 </tr>
 <tr>
 <td>SYTO 16 labeling reagent</td>
 <td>Invitrogen</td>
 <td> S7578</td>
 </tr>
 <tr>
 <td>Fiber-optic faceplate</td>
 <td>Edmund Optics</td>
 <td>NT55-142</td>
 </tr>
 <tr>
 <td>Fiber-optic taper</td>
 <td>Edmund Optics</td>
 <td>NT55-134</td>
 </tr>
 <tr>
 <td>Prisms</td>
 <td>Edmund Optics</td>
 <td>NT47-626, NT45-403</td>
 </tr>
 <tr>
 <td>Filters</td>
 <td>Edmund Optics</td>
 <td>NT39-417</td>
 </tr>
 <tr>
 <td>PDMS Elastomers</td>
 <td>Dow Corning</td>
 <td>Slygard 184</td>
 </tr>
 </tbody>
</table>

lensless fluorescent microscopy on a chip

On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for high-throughput screening applications. This emerging technology platform has the potential to eliminate the need for bulky and/or costly optical components through the help of novel theories and digital reconstruction algorithms. Along the same lines, here we demonstrate an on-chip fluorescent microscopy modality that can achieve e.g., &lt;4&mu;m spatial resolution over an ultra-wide field-of-view (FOV) of &gt;0.6-8 cm2 without the use of any lenses, mechanical-scanning or thin-film based interference filters. In this technique, fluorescent excitation is achieved through a prism or hemispherical-glass interface illuminated by an incoherent source. After interacting with the entire object volume, this excitation light is rejected by total-internal-reflection (TIR) process that is occurring at the bottom of the sample micro-fluidic chip. The fluorescent emission from the excited objects is then collected by a fiber-optic faceplate or a taper and is delivered to an optoelectronic sensor array such as a charge-coupled-device (CCD). By using a compressive-sampling based decoding algorithm, the acquired lensfree raw fluorescent images of the sample can be rapidly processed to yield e.g., &lt;4&mu;m resolution over an FOV of &gt;0.6-8 cm2. Moreover, vertically stacked micro-channels that are separated by e.g., 50-100 &mu;m can also be successfully imaged using the same lensfree on-chip microscopy platform, which further increases the overall throughput of this modality. This compact on-chip fluorescent imaging platform, with a rapid compressive decoder behind it, could be rather valuable for high-throughput cytometry, rare-cell research and microarray-analysis.

Watch this Scientific Journal Video about Lensless Fluorescent Microscopy on a Chip at JoVE.com

Lensless Fluorescent Microscopy on a Chip

A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., &#62;0.6-8 cm2 with &#60;4&#956;m resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.

On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for ...

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17.5K Views.  University of California, Los Angeles. A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with 