December 22nd, 2011
•Visualizing protein samples by negative stain electron microscopy (EM) has become a popular structural analysis method. It is useful for quantitative structural analysis, such as calculating a 3D reconstruction of the molecules being studied, and also for qualitative examination of the quality of protein preparations. In this article we present detailed protocols for preparing the EM grids, staining the sample and visualizing the sample in an electron microscope. Novice users can follow these protocols easily and to utilize negative stain EM as a routine assay, in addition to other biochemical assays, for evaluating their protein samples.
Tags
Related Videos
Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
Cell Co-culture Patterning Using Aqueous Two-phase Systems
Preparation of Hydroxy-PAAm Hydrogels for Decoupling the Effects of Mechanotransduction Cues
Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes