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Antileishmanial筛选对细胞内的寄生虫救援和转化实验

22.1K Views

12:22 min

December 30th, 2012

DOI :

10.3791/4054-v

December 30th, 2012


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70 THP1 Antileishmanial

此视频中的章节

0:05

Title

2:44

Seeding and Differentiation of THP1 Macrophages in a 96-well Plate and 16-chamber Glass Culture Slide

4:13

Infection of the Transformed THP1 Cells with Leishmania donovani Promastigotes

5:43

Treatment of Infected Macrophages with Test Drugs

6:43

Staining, Imaging and Image Analysis for Quantification of Infection and Drug Treatment Effect

8:09

Parasite Rescue & Transformation Assay: Controlled Lysis of Parasite-infected Macrophages

8:59

Quantitative Analysis of Transformed Promastigotes

9:22

Results: % Infectivity and Parasite Survival in the Presence or Absence of Drug

10:41

Conclusion

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