A Parasite Rescate y Transformación de ensayo para la detección antileishmanial contra intracelular

2:44

Seeding and Differentiation of THP1 Macrophages in a 96-well Plate and 16-chamber Glass Culture Slide

4:13

Infection of the Transformed THP1 Cells with Leishmania donovani Promastigotes

5:43

Treatment of Infected Macrophages with Test Drugs

6:43

Staining, Imaging and Image Analysis for Quantification of Infection and Drug Treatment Effect

8:09

Parasite Rescue & Transformation Assay: Controlled Lysis of Parasite-infected Macrophages

8:59

Quantitative Analysis of Transformed Promastigotes

9:22

Results: % Infectivity and Parasite Survival in the Presence or Absence of Drug

10:41

Conclusion