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JoVE Journal

Neuroscience

This content is Open Access.

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

17.8K Views

06:30 min

July 26th, 2012

July 26th, 2012

17,802 Views

0:05

Title

1:49

Preparation: DNA Solution and Needles

2:10

Preparation: Surgery Before DNA Injection and Electroporation

3:25

Surgery: DNA Injection and Electroporation

5:01

Surgery: Post-surgical Evaluation and Tissue Processing

5:28

Representative Results

6:06

Conclusion

This article describes in detail a protocol to electroporate in utero the cerebral cortex and the hippocampus at E14.5 in mice. We also show that this is a valuable method to study dendrites and spines in these two cerebral regions.

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