Deposition of Lipids from Organic Solvents: Classical Protocol
3:13
Electroformation of Giant Liposomes
5:19
Deposition of Lipids by Controlled Dehydration of Small Liposomes
7:10
Results: Electroformation of Giant Liposomes from Small Liposome Dispersions is Compatible with Reconstituted Ion Channel Function
8:32
Conclusion
Reconstituting functional membrane proteins into giant liposomes of defined composition is a powerful approach when combined with patch-clamp electrophysiology. However, conventional giant liposome production may be incompatible with protein stability. We describe protocols for producing giant liposomes from pure lipids or small liposomes containing ion channels.
