<a href="https://www.jove.com/video/50175">最小红斑剂量（MED）测试</a> 卡罗琳·赫克曼1，2，杰奎琳·克洛斯3，艾米本森2，德博拉·鲁尼2，Teja公司MUNSHI 1，苏珊D.达罗1，克利福德玻璃市4，沙龙L.曼尼5，大卫·奥斯林2雷切尔钱德勒 癌症预防与控制计划， 宾夕法尼亚大学 ， 福克斯蔡斯癌症中心的精神病学系，2，3， 德雷克塞尔大学心理学系，4医学系， 福克斯蔡斯癌症坎特 ，5癌症预防与控制计划， 癌症研究所新泽西州 本文介绍了如何进行最小红斑量（MED）测试，以确定定义的最低剂量的紫外线照射，这将导致红斑（燃烧）时给予个体。 <a href="https://www.jove.com/video/50344">测量细胞内Ca 2 +的变化在人类精子的4项技术：传统的荧光，停流荧光，流式细胞术及单细胞成像</a> 马塔埃斯佩兰萨·马丁内斯1，奥马尔·何塞1，宝琳娜托雷斯·罗德里格斯1，亚历杭德拉·索利斯-洛佩兹1，安娜A.桑切斯Tusie 1，Yoloxochitl桑切斯-格瓦拉1，马塞拉B. TREVINO 2，克劳迪亚L. TREVINO 1 1阿雅遗传学德尔发展ŸFisiología分子， 研究所德Biotecnología墨西哥国立自治大学 ，数学和科学系， <stroNG&gt;爱迪生州立学院细胞内Ca 2 +动态精子生理学和Ca 2 +敏感的荧光染料构成一个多功能的工具来研究它们是非常重要的。被用于的人口实验（荧光和停流荧光）和单细胞实验（流式细胞仪和单细胞成像），跟踪时空的[Ca 2 +]在人类精子细胞中的变化。 <a href="https://www.jove.com/video/50492">空气过滤设备，包括非织造网格静电重组蜘蛛丝蛋白</a> 格雷戈尔郎，斯蒂芬Jokisch，托马斯·沙伊贝尔生物材料研究组， 拜罗伊特大学 蜘蛛丝纤维显示出非凡的机械性能。工程蛛diadematus素蛋白4，（eADF4）可以被加工成非织造网格，使用静电纺丝。她的<html> e，则eADF4的无纺网格被用来改善空气过滤装置的性能。 <a href="https://www.jove.com/video/4439">体外实时成像在小鼠神经上皮单细胞分裂</a> Karolina的Piotrowska NITSCHE 1,2，塔玛拉CASPARY 1 1 <a href="/institutions/NA-north-america/US-united-states/GA-georgia/24832-emory-university">， 埃默里大学医学院</a> ，2部实验胚胎学， <a href="/institutions/NA-north-america/US-united-states/GA-georgia/24832-emory-university">人类遗传学系</a> IGAB波兰科学院 在这里，我们开发体外实时成像跟踪单E8.5的小鼠神经上皮细胞分裂所必需的工具 <a href="https://www.jove.com/video/50047">采用先进的3D荧光显微镜定量分析自噬</a> 帐篷“春A. Changou 1，蒂娜L.沃尔夫森2，Balpreet辛格阿卢瓦利亚3，理查德J.大胆4，兴吉恩西贡5，弗兰克YS庄6 生物化学与分子医学系， 生物光子学科学技术中心2 NSF生物光子学科学技术， 美国加州大学戴维斯分校 ，美国国家科学基金会生物光子学科学技术中心， 特罗姆瑟大学 ，外科部， 大学NSF中心加州戴维斯分校，戴维斯分校 ， 加州大学生物化学，生物化学与分子医学系， 美国加州大学戴维斯分校的 5部细胞自噬是一个无处不在的进程，使细胞蛋白质降解和回收，奥尔加内勒斯。我们采用先进的荧光显微镜可视化和量化小，但必要的，与诱导自噬相关的物理变化，包括自噬体和溶酶体的形成和分布，以及他们融合成auto​​lysosomes。

may 2013: this month in jove

下面就来看看即将在<a href="http://www.jove.com/archive/75">2013年5月发行</a>的“朱庇特-杂志可视化实验。 什么来到第一：先有鸡还是先有蛋？这个基本问题缺乏再现：精子的重要组成部分。之前，任何事情都有可能发生，精子细胞必须游到卵子和融合。这些事件在很大程度上是依赖于钙离子（Ca 2 +的）介导的信号转导通路。因此，研究精子功能的影响，它是有用的测量Ca 2 +浓度在精子的变化。这可以促进钙敏感的荧光染料，其中： <a href="http://www.jove.com/video/50344">马塔·马丁内斯等。</a>使用四个荧光技术监测Ca 2 +的人精子：常规荧光光度法，动力学停流荧光光度法，流式细胞术，和单细胞成像。 移动ALO纳克的男性生殖道的另一部分，我们配备了一篇文章，关于前列腺癌，在美国的男性癌症死亡的主要原因之一。在人类前列腺癌细胞，这种酶叫做精氨酸脱亚胺酶（ADI）最近证实诱导自噬，细胞死亡机制的细胞基本上吃自己的组件。为了进一步研究这种机制， <a href="http://www.jove.com/video/50047">Changou 等</a>开发基于成像的方法，用定量的三维荧光显微镜，让他们能够精确地跟踪形态学变化，细胞发生自噬。这项技术将有助于研究人员研究前列腺癌的潜在疗法。 在<a href="http://www.jove.com/bioengineering">朱庇特生物工程</a> ，过去的文章中已经提出的方法从蜘蛛丝绸生产腺体隔离，生产重组蜘蛛丝蛋白在细菌，和纺纯化PROTE成纤维潜在的生物医学应用（ <a href="http://www.jove.com/video/2382">杰弗里等人 ，2011</a> ; <a href="http://www.jove.com/video/4191">夏等人，2012</a> ）的插件。这个月， <a href="http://www.jove.com/video/50492">郎等人</a>提出了一种新的应用程序蜘蛛丝：空气过滤装置的静电重组蜘蛛丝蛋白与非织造网眼。如果蜘蛛丝涂有一层商业过滤器，其过滤效率可以得到改善。作者展示了如何electrospin纤维，对待非织造丝绸网，并利用扫描电镜（SEM）分析的网格。最后，作者展示了透气性和过滤效率。 <a href="http://www.jove.com/video/50175">赫克曼等人</a>在<a href="http://www.jove.com/ctm">朱庇特的临床和转化医学</a> ，为确定最低剂量ultravi的展示出一种方法OLET光会引起红斑（晒斑），在一个单独的。紫外线灯经常被用来治疗各种皮肤状况，如牛皮癣，痤疮，湿疹，因为不是所有的人都同样对紫外线敏感，这种方法可以帮助确定合适的剂量管理。甲Daavlin补丁是用来控制在皮肤上的UV曝光的面积和持续时间。第二天，皮肤颜色的变化进行评估，以确定引起红斑所需的紫外线剂量最低。 在<a href="http://www.jove.com/neuro">朱庇特的神经</a> ， <a href="http://www.jove.com/video/4439">Piotrowska NITSCHE CASPARY</a>演示如何培养小鼠胚胎神经上皮片和各种荧光标记进行实时成像。这种方法使研究人员能够监测细胞的行为，如单细胞分裂，就地和实时。 5月份刚喝了朱庇特的一些亮点预览。访问该网站看到全长的文章，加上许多，在朱庇特：[可视化实验。

Watch this Scientific Journal Video about May 2013: This Month in JoVE at JoVE.com

May 2013: This Month in JoVE

这里有一些亮点从2013年5月发行的杂志可视化实验（朱庇特）。

2013年5月：这个月在朱庇特

Here's a look at what's coming up in the May 2013 issue of JoVE - The Journal of Visualized Experiments.
What came first: the chicken or the egg? This ...

Without Section

8.2K 次观看.  Massachusetts Eye and Ear. 这里有一些亮点从2013年5月发行的杂志可视化实验（朱庇特）。

视频: May 2013: This Month in JoVE

December 2011: This Month in JoVE

The Journal of Visualized Experiments (JoVE) closes 2011 with a December issue that begins by honoring World's AIDS Day with an article that deals with visualizing the structure of HIV envelope glycoproteins, which are a critical component of the viral infection pathway.
One key method for determining virion envelope structure is cryo-electron tomography. Prior to using this technique, soluble virus must be vitrified on a gold grid - a process that involves adding gold to a viral suspension, transferring that suspension to the grid, and plunging the grid into liquid ethane. The virus particles, present in a thin film of liquid atop the grid, are frozen at a rate greater than 100,000 K per second. Following vitrification, collected samples are loaded into the electron microscope and precisely defined regions on the grid are hit with a focused electron beam, while the grid is tilted. The tilt series that results from these imaging sessions is comprised of hundreds of individual images, which must be aligned and averaged to resolve individual envelope proteins. This computationally intense process often requires computer clusters, capable of remote-access parallel processing, like NIH's Biowulf.
Once completed, 3D models can not only provide insight into the structure of envelope glycoproteins themselves, but can also reveal the manner in which they interact other proteins, like the neutralizing antibody B12, which is information that can greatly influence vaccine design.
This article, from the laboratory of Dr. Sriram Subramaniam involves contributions from twenty authors, including not only graduates students and postdocs, but also middle and highschool students, showing that you are never too young to make an impact in the fight against a disease that has infected over 60 million people, worldwide.
In Clinical and Translational Medicine, JoVE presents an article from the University College London and the University of Oxford that investigates the physiological correlates of nociception, or pain sensation, in infants. Typically, infants respond to noxious stimuli by crying and changing their facial expressions - reactions considered to be autonomic and reliant on subcortical regions of the brain.
In order to understand the involvement of the cerebral cortex and spinal cord in infant nociception our authors first prepare infants for EEG and EMG recordings. They also set up physiological monitoring and video recording, so that the infant's facial expressions can be linked to physiological data. Following assessment of infant responses to neutral tactile stimuli, the baby is subjected to heel lancing - a clinical procedure used to obtain blood samples from infants. After data processing, electrical potentials can be compared between noxious and non-noxious stimuli in these infants, which will hopefully lead to meaningful conclusions, regarding how nociceptive information is interpreted by newborns.
JoVE's Bioengineering section takes a more clinical focus in December with an article from the University of Wisconsin, Madison, which involves the fabrication of a compartmentalized microfluidic device for studying cancer stem cell migration. Following device design, master molds are created by photolithography for the application of poly dimethyl siloxane (PDMS)- an organosilicon compound that is transparent and biologically inert - making it suitable for microfluidics applications with live cells. Specifically, our authors load tumor stem cells derived from multiform glioblastoma, a highly malignant brain cancer, into the compartmentalized devices and then place them in a specialized imaging system that acquires data over a few days. 
Time lapse video from these authors show that cancer stem cells are able to regulate their morphology in the size-constrained microfluidic channels much like they would in the interstitial space, and fluidic isolation made possible by chambers like these facilitates drug screening for potential therapeutics that can affect cancer stem cell migration.
Late on in the month, JoVE travels to France, where investigators from the Institute Pasteur and the Centre National de la Recherche Scientifique, illustrate a method for inducing expression of channel-rhodopsin, a light-activated ion channel, into the olfactory bulb of mice, as well as subsequent neuronal stimulation using a miniature light emitting diode (LED).
Our authors give detailed step-by-step instructions for preparing injection needles, and for precise injection of virus into the rostral migratory stream, a site of migration for adult born neuroblasts. Furthermore, they show how miniature LED stimulation devices are fabricated, calibrated, and implanted atop a cranial window prepared over the bulb. In particular, our authors are interested in the way that adult born neurons integrate into existing olfactory neuronal circuits, and they show that LED stimulation in awake behaving animals, can lead to activation of virally-tranduced neuroblasts in the olfactory bulb, which have migrated from the rostral migratory stream.
These video-articles comprise JoVE's highlights for the month of December. Other noteworthy upcoming articles illustrate methods for patterning conductive inks into microelectrodes, measuring cytosolic calcium in contractile lymphatics, and imaging neuronal responses to pheromones in the vomeronasal organ.

Here are some highlights from the December 2011 Issue of Journal of Visualized Experiments (JoVE).

这在朱庇特12月

The Journal of Visualized Experiments (JoVE) closes 2011 with a December issue that begins by honoring World's AIDS Day with an article that deals with ...

科研

JoVE Journal

生物学

October 2012: This Month in JoVE

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE). Gr&oslash;nlund et al. demonstrate how to isolate and analyze specific cell types in plant leaves expressing green fluorescent protein (GFP) using fluorescence-activated cell sorting (FACS). This method overcomes the interfering fluorescence of the chlorophyll found in leaves, and distinguishes GFP-expressing protoplasts from non-GFP protoplasts. In JoVE Neuroscience, Babona-Pilipos et al. demonstrate how to construct a chamber for measuring galvanotaxis, or cell migration within an electric field. Through time-lapse imaging and image analysis, the authors can study the migratory behavior of neural precursor cells in an electric field, which may lead to the use of electrical stimulation to direct neural precursors to sites of injury or disease. Articles involving microfluidic platforms have a significant publication history in JoVE. Harris et al. have released three articles, which involve a microfluidic device for separating axons from neuronal cell bodies. This month in JoVE Neuroscience, Higashimori et al. use this microfluidic platform to investigate the interactions between neuronal axons and glial cells, which are critical for the physiological function of the nervous system. In JoVE Bioengineering, two research groups demonstrate the novel bioadhesive properties of chitosan-a polymer derived from chitin, which is found in fungal cell walls or in the exoskeletons of crustaceans and insects. Chitosan is used in many industrial and agricultural applications, and bioengineers are also finding uses for it in surgical applications. Foster et al. have developed a laser-activated surgical film called Surgilux, which combines chitosan with indocyanine green ICG), a photosensitive dye. This surgical film binds strongly to tissue, such as muscle, after laser irradiation. Lauto et al. developed a surgical film that combines chitosan with the photoactive dye, rose Bengal. This novel film also binds strongly to tissues, such as intestine, after it is irradiated. These adhesive films are biocompatible and may potentially be used in various surgical procedures in place of sutures. In JoVE Clinical and Translational Medicine, Fiema et al. demonstrate a high-throughput technique for validating biomarkers in graft vs. host disease, a common and life-threatening complication of cell or tissue transplantation. The authors use commercially available ELISAs to analyze multiple proteins in sequential fashion. In JoVE Immunology and Infection, Keyel et al. demonstrate how to measure the real-time kinetics of immune cell responses to bacterial toxins using high-speed live cell microscopy. This method can be used to show how immune cells respond to bacterial toxins. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response. In JoVE Applied Physics, Borisenko et al. determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy in a synchotron radiation facility. By combining recent advances in synchotron radiation, surface science, and cryogenics, this method can gain a precise picture of the energy and momentum of electrons inside a solid, and address key questions in the field of condensed matter physics. This preview summarizes just a few of the notable video-articles available in the October 2012 issue of JoVE. For additional videos, please visit <a href="http://www.jove.com">www.jove.com</a>.

Here are some highlights from the October 2012 Issue of Journal of Visualized Experiments (JoVE).

2012年10月：这个月在朱庇特

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE).  Gr&oslash;nlund et al. demonstrate how to isolate and ...

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012年：年度回顾

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...