<a href="https://www.jove.com/video/50175">Eritema Dose (MED), testes mínimos</a> Carolyn J. Heckman 1, Rachel Chandler 2, Jacqueline D. Kloss 3, Amy Benson 2, Deborah Rooney 2, Teja Munshi 1, Susan D. Darlow 1, Clifford Perlis 4, Sharon L. Manne 5, David W. Oslin 2 1 de Prevenção do Câncer e do Programa de Controle, Fox Chase Cancer Center, 2 Departamento de Psiquiatria da Universidade da Pensilvânia, 3 Departamento de Psicologia da Universidade Drexel, 4 Departamento de Medicina, Fox Chase Cancer Canter, 5 de Prevenção do Câncer e do Programa de Controle, o Instituto do Câncer New Jersey Este artigo descreve como realizar o teste da dose eritematosa mínima (DEM), para determinaçãone a menor dose de radiação ultravioleta, que vai provocar eritema (queimadura), quando administrados a um indivíduo. <a href="https://www.jove.com/video/50344">Medindo Ca 2 + intracelular Mudanças no esperma humano, utilizando quatro técnicas: fluorometria convencional, Parado Fluorometria fluxo, citometria de fluxo e única célula de imagem</a> Esperanza Martínez Mata-1, Omar José 1, Paulina Torres-Rodríguez 1, Alejandra López Solís-1, Ana A. Sánchez-Tusie 1, Yoloxochitl Sánchez Guevara-1, Marcela B. Treviño 2, Claudia L. Treviño 1 1 Departamento de Genética del Desarrollo y Fisiologia Molecular, Instituto de Biotecnologia-Universidad Nacional Autónoma de México, 2 Departamento de Matemática e Ciências, <strong&gt; Edison State College Ca 2 + intracelular dinâmica são muito importantes na fisiologia de esperma e de Ca2 + sensíveis à corantes fluorescentes constituem uma ferramenta versátil para estudá-los. Experiências da população (fluorometria e parou fluorometria fluxo) e experimentos com células individuais (citometria de fluxo e de imagem única célula) são usados ​​para rastrear espaço-temporais [Ca 2 +] alterações nas células de esperma humano. <a href="https://www.jove.com/video/50492">Filtro de ar de dispositivos, incluindo malhas não tecidos de electrospun Proteínas Recombinantes de seda de aranha</a> Gregor Lang, Stephan Jokisch, Thomas Scheibel Biomaterials Research Group, da Universidade de Bayreuth Fibras de seda de aranha exibir extraordinárias propriedades mecânicas. Engineered Araneus fibroína 4 (eADF4) pode ser transformado em malhas não tecidos usando electrospinning. Seu<html> E, o eADF4 malhas, não tecidos são utilizados para melhorar o desempenho dos dispositivos de filtragem de ar. <a href="https://www.jove.com/video/4439">Ex vivo imagens ao vivo de divisões celulares simples no mouse neuroepithelium</a> Karolina Piotrowska-Nitsche 1,2, Tamara Caspary 1 1 <a href="/institutions/NA-north-america/US-united-states/GA-georgia/24832-emory-university">Departamento de Genética Humana, Emory University School of Medicine</a> , 2 Departamento de Embriologia Experimental, IGAB Polish Academy of Sciences Aqui vamos desenvolver as ferramentas necessárias para ex vivo de imagem in vivo para traçar divisões celulares individuais no rato E8.5 neuroepithelium <a href="https://www.jove.com/video/50047">Análise Quantitativa de Autophagy usando microscopia de fluorescência avançada 3D</a> tenda &quot;&gt; Chun A. Changou 1, Deanna L. Wolfson 2, Balpreet Singh Ahluwalia 3, Richard J. Negrito 4, Hsing-Jien Kung 5, Frank YS Chuang 6 1 Departamento de Medicina Bioquímica e Molecular, NSF Center for Biophotonics Ciência e Tecnologia, 2 NSF Center for Biophotonics Ciência e Tecnologia, da Universidade da Califórnia, em Davis, 3 NSF Center for Biophotonics Ciência e Tecnologia da Universidade de Tromsø, 4 Departamento de Cirurgia da Universidade da Califórnia, em Davis, 5 Department of Biological Chemistry, University of California, Davis, 6 Department of Biochemistry and Molecular Medicine, University of California, Davis Autophagy é um processo ubíquo que permite que as células de degradar proteínas e reciclar e organelles. Aplicamos microscopia de fluorescência para visualizar avançada e quantificar o pequeno, mas, alterações físicas essenciais associados com a indução de autofagia, incluindo a formação e distribuição de autofagossomas e lisossomas, e sua fusão em autolysosomes.

Não há conflitos de interesse declarados.

may 2013: this month in jove

Aqui está um olhar para o que está chegando no <a href="http://www.jove.com/archive/75">maio 2013 questão</a> de Jove - The Journal of Experiments visualizadas. O que veio primeiro: o ovo ou a galinha? Esta questão fundamental não tem um componente vital da reprodução: o esperma. Antes de qualquer coisa pode acontecer, uma célula de esperma deve nadar até o óvulo e fundir-se com ele. Estes eventos são muito dependentes vias de sinalização mediadas por íons de cálcio (Ca 2 +). Assim, para estudar a função espermática, é útil para medir alterações na concentração de Ca2 + em espermatozóides. Isto pode ser facilitado através de cálcio sensíveis corantes fluorescentes, que <a href="http://www.jove.com/video/50344">Mata-Martinez et ai.</a> usar em quatro técnicas fluorométricos para controlar a dinâmica de Ca 2 + no esperma humano: fluorometria convencional, o fluxo parou fluorometria, a citometria de fluxo e de imagem de uma única célula. Movendo along para outra parte do trato reprodutivo masculino, que apresentam um artigo sobre o câncer de próstata, uma das principais causas de morte por câncer em homens nos Estados Unidos. Em células humanas de cancro da próstata, uma enzima chamada arginina deiminase (ADI), foi recentemente demonstrado para induzir autofagia, um mecanismo de morte celular no qual uma célula essencialmente come os seus próprios componentes. Para estudar mais este mecanismo, <a href="http://www.jove.com/video/50047">Changou et al.</a> desenvolveu uma abordagem baseada em imagens através de microscopia de fluorescência quantitativa 3D, permitindo-lhes acompanhar precisamente alterações morfológicas como células sofrem autofagia. Esta técnica irá ajudar os pesquisadores a estudar potenciais terapias para o câncer de próstata. Em <a href="http://www.jove.com/bioengineering">Bioengenharia Jove</a> , artigos anteriores têm apresentado métodos para isolar as glândulas produtoras de seda das aranhas, produzindo proteínas da seda de aranha recombinantes em bactérias, e girando purificada proteins em fibras para potenciais aplicações biomédicas ( <a href="http://www.jove.com/video/2382">Jeffrey et al, 2011.</a> ; <a href="http://www.jove.com/video/4191">. Hsia et al, 2012</a> ). Este mês, <a href="http://www.jove.com/video/50492">. Lang et al</a> apresentar um novo pedido de seda de aranha: a fabricação de dispositivos de filtro de ar com um não tecido de malha de proteínas da seda de aranha recombinantes electrospun. Se os filtros comerciais são revestidos com uma camada de seda de aranha, a sua eficiência de filtragem pode ser melhorada. Os autores demonstram como electrospin as fibras, tratar as malhas de seda não tecidos, e analisar as malhas usando microscopia eleição (SEM). Finalmente, os autores demonstram permeabilidade ao ar e a eficiência do filtro. Em <a href="http://www.jove.com/ctm">Medicina Clínica e Translacional Jove</a> , <a href="http://www.jove.com/video/50175">Heckman et al.</a> demonstrar um método para determinar a menor dose de ultraviolet luz que irá causar eritema (queimadura solar), em um indivíduo. Luz UV é usada para tratar várias condições da pele, tais como psoríase, acne e eczema, porque nem todas as pessoas são igualmente sensíveis à luz UV, este método pode ajudar a determinar a dose apropriada para administrar. Um remendo Daavlin é usado para controlar tanto a área ea duração da exposição à radiação UV sobre a pele. No dia seguinte, as variações na cor da pele são avaliadas para determinar a menor dose de UV requerida para provocar eritema. Em <a href="http://www.jove.com/neuro">JOVE Neuroscience</a> , <a href="http://www.jove.com/video/4439">Piotrowska-Nitsche e Caspary</a> demonstrar como fatias cultura de neuroepithelium embrionárias de camundongos e realizar imagens ao vivo de vários marcadores fluorescentes. Este método permite ao pesquisador monitorar o comportamento das células, tais como divisões celulares individuais, in situ e em tempo real. Você teve apenas uma pré-visualização de alguns dos destaques de Jove para o mês de maio. Visite o site para ver os artigos completos, além de muitas outras, em Jove: The Journal of Experiments visualizadas.

Watch this Scientific Journal Video about May 2013: This Month in JoVE at JoVE.com

May 2013: This Month in JoVE

Aqui estão alguns destaques da edição de maio 2013 Journal of Experiments visualizar (Jove).

Maio 2013: Este mês na JOVE

Here's a look at what's coming up in the May 2013 issue of JoVE - The Journal of Visualized Experiments.
What came first: the chicken or the egg? This ...

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8.2K visualizações.  Massachusetts Eye and Ear. Aqui estão alguns destaques da edição de maio 2013 Journal of Experiments visualizar (Jove).

Vídeo: May 2013: This Month in JoVE

December 2011: This Month in JoVE

The Journal of Visualized Experiments (JoVE) closes 2011 with a December issue that begins by honoring World's AIDS Day with an article that deals with visualizing the structure of HIV envelope glycoproteins, which are a critical component of the viral infection pathway.
One key method for determining virion envelope structure is cryo-electron tomography. Prior to using this technique, soluble virus must be vitrified on a gold grid - a process that involves adding gold to a viral suspension, transferring that suspension to the grid, and plunging the grid into liquid ethane. The virus particles, present in a thin film of liquid atop the grid, are frozen at a rate greater than 100,000 K per second. Following vitrification, collected samples are loaded into the electron microscope and precisely defined regions on the grid are hit with a focused electron beam, while the grid is tilted. The tilt series that results from these imaging sessions is comprised of hundreds of individual images, which must be aligned and averaged to resolve individual envelope proteins. This computationally intense process often requires computer clusters, capable of remote-access parallel processing, like NIH's Biowulf.
Once completed, 3D models can not only provide insight into the structure of envelope glycoproteins themselves, but can also reveal the manner in which they interact other proteins, like the neutralizing antibody B12, which is information that can greatly influence vaccine design.
This article, from the laboratory of Dr. Sriram Subramaniam involves contributions from twenty authors, including not only graduates students and postdocs, but also middle and highschool students, showing that you are never too young to make an impact in the fight against a disease that has infected over 60 million people, worldwide.
In Clinical and Translational Medicine, JoVE presents an article from the University College London and the University of Oxford that investigates the physiological correlates of nociception, or pain sensation, in infants. Typically, infants respond to noxious stimuli by crying and changing their facial expressions - reactions considered to be autonomic and reliant on subcortical regions of the brain.
In order to understand the involvement of the cerebral cortex and spinal cord in infant nociception our authors first prepare infants for EEG and EMG recordings. They also set up physiological monitoring and video recording, so that the infant's facial expressions can be linked to physiological data. Following assessment of infant responses to neutral tactile stimuli, the baby is subjected to heel lancing - a clinical procedure used to obtain blood samples from infants. After data processing, electrical potentials can be compared between noxious and non-noxious stimuli in these infants, which will hopefully lead to meaningful conclusions, regarding how nociceptive information is interpreted by newborns.
JoVE's Bioengineering section takes a more clinical focus in December with an article from the University of Wisconsin, Madison, which involves the fabrication of a compartmentalized microfluidic device for studying cancer stem cell migration. Following device design, master molds are created by photolithography for the application of poly dimethyl siloxane (PDMS)- an organosilicon compound that is transparent and biologically inert - making it suitable for microfluidics applications with live cells. Specifically, our authors load tumor stem cells derived from multiform glioblastoma, a highly malignant brain cancer, into the compartmentalized devices and then place them in a specialized imaging system that acquires data over a few days. 
Time lapse video from these authors show that cancer stem cells are able to regulate their morphology in the size-constrained microfluidic channels much like they would in the interstitial space, and fluidic isolation made possible by chambers like these facilitates drug screening for potential therapeutics that can affect cancer stem cell migration.
Late on in the month, JoVE travels to France, where investigators from the Institute Pasteur and the Centre National de la Recherche Scientifique, illustrate a method for inducing expression of channel-rhodopsin, a light-activated ion channel, into the olfactory bulb of mice, as well as subsequent neuronal stimulation using a miniature light emitting diode (LED).
Our authors give detailed step-by-step instructions for preparing injection needles, and for precise injection of virus into the rostral migratory stream, a site of migration for adult born neuroblasts. Furthermore, they show how miniature LED stimulation devices are fabricated, calibrated, and implanted atop a cranial window prepared over the bulb. In particular, our authors are interested in the way that adult born neurons integrate into existing olfactory neuronal circuits, and they show that LED stimulation in awake behaving animals, can lead to activation of virally-tranduced neuroblasts in the olfactory bulb, which have migrated from the rostral migratory stream.
These video-articles comprise JoVE's highlights for the month of December. Other noteworthy upcoming articles illustrate methods for patterning conductive inks into microelectrodes, measuring cytosolic calcium in contractile lymphatics, and imaging neuronal responses to pheromones in the vomeronasal organ.

Here are some highlights from the December 2011 Issue of Journal of Visualized Experiments (JoVE).

Em dezembro em JOVE

The Journal of Visualized Experiments (JoVE) closes 2011 with a December issue that begins by honoring World's AIDS Day with an article that deals with ...

Research

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Biologia

October 2012: This Month in JoVE

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE). Gr&oslash;nlund et al. demonstrate how to isolate and analyze specific cell types in plant leaves expressing green fluorescent protein (GFP) using fluorescence-activated cell sorting (FACS). This method overcomes the interfering fluorescence of the chlorophyll found in leaves, and distinguishes GFP-expressing protoplasts from non-GFP protoplasts. In JoVE Neuroscience, Babona-Pilipos et al. demonstrate how to construct a chamber for measuring galvanotaxis, or cell migration within an electric field. Through time-lapse imaging and image analysis, the authors can study the migratory behavior of neural precursor cells in an electric field, which may lead to the use of electrical stimulation to direct neural precursors to sites of injury or disease. Articles involving microfluidic platforms have a significant publication history in JoVE. Harris et al. have released three articles, which involve a microfluidic device for separating axons from neuronal cell bodies. This month in JoVE Neuroscience, Higashimori et al. use this microfluidic platform to investigate the interactions between neuronal axons and glial cells, which are critical for the physiological function of the nervous system. In JoVE Bioengineering, two research groups demonstrate the novel bioadhesive properties of chitosan-a polymer derived from chitin, which is found in fungal cell walls or in the exoskeletons of crustaceans and insects. Chitosan is used in many industrial and agricultural applications, and bioengineers are also finding uses for it in surgical applications. Foster et al. have developed a laser-activated surgical film called Surgilux, which combines chitosan with indocyanine green ICG), a photosensitive dye. This surgical film binds strongly to tissue, such as muscle, after laser irradiation. Lauto et al. developed a surgical film that combines chitosan with the photoactive dye, rose Bengal. This novel film also binds strongly to tissues, such as intestine, after it is irradiated. These adhesive films are biocompatible and may potentially be used in various surgical procedures in place of sutures. In JoVE Clinical and Translational Medicine, Fiema et al. demonstrate a high-throughput technique for validating biomarkers in graft vs. host disease, a common and life-threatening complication of cell or tissue transplantation. The authors use commercially available ELISAs to analyze multiple proteins in sequential fashion. In JoVE Immunology and Infection, Keyel et al. demonstrate how to measure the real-time kinetics of immune cell responses to bacterial toxins using high-speed live cell microscopy. This method can be used to show how immune cells respond to bacterial toxins. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response. In JoVE Applied Physics, Borisenko et al. determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy in a synchotron radiation facility. By combining recent advances in synchotron radiation, surface science, and cryogenics, this method can gain a precise picture of the energy and momentum of electrons inside a solid, and address key questions in the field of condensed matter physics. This preview summarizes just a few of the notable video-articles available in the October 2012 issue of JoVE. For additional videos, please visit <a href="http://www.jove.com">www.jove.com</a>.

Here are some highlights from the October 2012 Issue of Journal of Visualized Experiments (JoVE).

Outubro de 2012: Este mês, em JoVE

Here are some highlights from the October 2012 issue of Journal of Visualized Experiments (JoVE).  Gr&oslash;nlund et al. demonstrate how to isolate and ...

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: Um ano em análise

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...