Fixation of Protein-protein Interactions by Formaldehyde Cross-linking in Living Cells
4:07
Purification of Strep-tagged Membrane Protein
5:24
Purification of Strep-tag Membrane Bait Protein and SDS-PAGE
7:43
Confirmation of Interaction Partners
8:53
Results: Membrane SPINE Analysis
10:33
Conclusion
A biochemical approach is described to identify in vivo protein-protein interactions (PPI) of membrane proteins. The method combines protein cross-linking, affinity purification and mass spectrometry, and is adaptable to almost any cell type or organism. With this approach, even the identification of transient PPIs becomes possible.
