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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

DOI :

10.3791/50883-v

January 12th, 2014

January 12th, 2014

8,075 Views

1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534, University of Lyon

We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

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