JoVE Journal

Immunology and Infection

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方法评估细胞毒性的可燃烟草产品的准备和免疫抑制

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09:25 min

January 10th, 2015

January 10th, 2015


0:05

Title

1:40

Intracellular Staining and Flow Cytometry

3:52

K562 Killing Assay

6:33

Results: Representative Cytotoxic and Immunosuppressive Effects of Combustible Tobacco Product Preparations

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Conclusion

Using optimized human peripheral blood mononuclear cell (PBMC) ex vivo assays, we showed that a combustible tobacco product preparation markedly suppresses receptor-mediated intracellularly secreted cytokines and cytolytic ability of effector PBMCs. These rapid assays may be useful in product evaluation and understanding the potential long-term effects of tobacco exposure.

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