The authors would like to thank Professor Yuzuru Takashima at the University of Arizona for discussions on optical imaging, Mr. Karl Urbanek for assistance with high power lasers, and Max Yuen for discussions of Brownian motion. The authors send further thanks to Professor Kenneth Crozier at Harvard University for helpful discussions on optical trapping experiments. Funding was provided in part by the United States National Science Foundation (award number 1028372).

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The authors have nothing to disclose.

The NOCB combines the strong trapping forces and small trap size of plasmonic approaches with the capability to transport particles, long available only for conventional focused-beam techniques. Unique to the NOCB, the trapping and transport properties of the system are a result of surface patterning and not of shaping the illumination beam. Provided the illumination is bright enough and its polarization or wavelength can be modulated, particles can be held or moved in complicated protocols on the surface. We have demons...

捕获，审讯和操纵单个纳米颗粒在纳米技术越来越重要。光学镊子已成为在那里他们已启用突破实验如单DNA分子4和机械性能的测定在分子生物学1-4，化学5-7和纳米组装7-10，实验一特别成功操作技术细胞通过它们的光学性质11,12排序。这些前沿的发现开辟了更小的系统研究，他们让路新切实有益的产品和技术工程。反过来，这种趋势驱动需要新的技术来操纵更小，更基本的粒子。此外，还有一个推构建&#39;上实验室一个芯片的设备中，以便使化学和生物学检验出的更便宜和更小的封装执行这些功能实验室和进入该领域的医疗和其他目的13,14。 不幸的是，传统的光学捕获（COT）不能满足所有的纳米技术的日益增长的需求。 COT操作上使用高数值孔径（NA）的物镜，使激光光到紧聚焦，产生在光强度和高梯度的电磁场能量的局部峰值的机制。这些能量密度梯度施加的光漫射粒子的净力通常吸引他们在朝向焦点的中心。捕获更小的颗粒要求更高的光功率或突出重点。然而，光的聚焦光束服从衍射原理，这限制了焦斑的最小尺寸和在能量密度梯度了一个上限。有效的COT不能捕获的小物件，和COT有麻烦了密集的颗粒之间进行区分，一个诱捕分辨率：这有两个直接后果限制被称为“胖手指”的问题。此外，实现多个粒子捕获用COT需要光束转向光学器件或空间光调制器，组分急剧增加的光学捕获系统的成本和复杂性的系统。 规避光的常规聚焦光束的根本局限性的一种方法，所述传播在远场，是改为利用光的电磁能量的梯度，在近场。近场呈指数衰减远离电磁场源，这意味着不仅是它高度局部化到这些源，但它也表现出非常高的梯度在其能量密度。纳米金属谐振器，如蝴蝶结孔，纳米柱，和C形雕刻的近场，已被证明表现出近INFR非凡浓度的电磁能量，通过金，银的等离子体激元的作用的进一步增强的ARED和光的波长。这些谐振器已被用于捕集非常小的微粒子以高效率和分辨率15-22。虽然这一技术已被证明在捕集小颗粒有效的，它也被证明在其上可感知的范围，这是必要的，如果近场的系统与远场的系统或微流体接口输送粒子的能力是有限的。 最近，我们的组提出了解决这一问题。当谐振器被放置得很近，颗粒原则上可以从一个近场光阱到下迁移，而从表面释放。传输的方向可以，如果相邻的陷阱可打开和关闭分别确定。的三个或更多个可寻址的谐振器，其中每一个谐振器是一个偏振或光不同波长从它的邻居敏感的线性阵列，为光输送带，输送nanoparti克莱斯超​​过一个芯片上的距离为几微米。 所谓“纳米光传送带”（NOCB）是其中等离子谐振器诱捕方案独一无二的，因为它不仅可以在地方举办的颗粒，但它也可以沿着图案的轨道移动在高速，聚集或分散颗粒，混合排队它们，甚至对它们进行排序按性质，如他们的流动性23。所有这些功能都通过调制照明的偏振或波长，而无需光束转向光学器件的控制。作为近场光学陷阱，该NOCB诱捕分辨率比常规聚焦光束的光阱的更高，因此它可以在靠近颗粒之间区分;因为它采用了金属纳米结构，以光集中于一个俘获很好，这是功率效率，并且不需要昂贵的光学组件，例如一个高NA物镜。此外，许多NOCBs可以并行地操作时，在高的填充巢穴sity，在同一基板上，并且功率为1W可以驱动超过1200孔23。 我们最近展示了第一个偏振驱动NOCB，顺利推进纳米粒子来回沿着4.5微米的轨道24。在这篇文章中，我们提出必要的设计和制造设备的步骤，光激活并繁殖运输试验。我们希望使这一技术更广泛的应用将有助于弥补微流控，远场光学和纳米器件与实验之间的大小差距。

<table border="0" cellpadding="0" cellspacing="0">
	<tbody>
		<tr>
			<td>HSQ e-beam resist</td>
			<td>Dow Corning</td>
			<td>XR-1541-006</td>
			<td></td>
		</tr>
		<tr>
			<td>PMMA</td>
			<td>MicroChem</td>
			<td>950A2 M230002</td>
			<td></td>
		</tr>
		<tr>
			<td>Fast curing optical adhesive</td>
			<td>Norland Optical Adhesive</td>
			<td>NOA 81</td>
			<td></td>
		</tr>
		<tr>
			<td>Fluorescent carboxyl microspheres</td>
			<td>Bangs Laboratories</td>
			<td>FC02F, FC03F</td>
			<td></td>
		</tr>
		<tr>
			<td>Fluorescent carboxylate-modified microspheres</td>
			<td>Molecular Probes</td>
			<td>F-8888</td>
			<td></td>
		</tr>
		<tr>
			<td>Quartz slide</td>
			<td>SPI Supplies</td>
			<td>1020-AB</td>
			<td></td>
		</tr>
		<tr>
			<td>Inverted fluorescent microscope</td>
			<td>Nikon</td>
			<td>ECLIPSE TE2000-U</td>
			<td></td>
		</tr>
		<tr>
			<td>Nd:YAG laser</td>
			<td>Lightwave Electronics</td>
			<td>221-HD-V04</td>
			<td></td>
		</tr>
		<tr>
			<td>sCMOS camera</td>
			<td>PCO</td>
			<td>EDGE55</td>
			<td></td>
		</tr>
		<tr>
			<td>CCD camera</td>
			<td>Watec</td>
			<td>WAT-120N</td>
			<td></td>
		</tr>
		<tr>
			<td>Zero-order half-wave plate</td>
			<td>Thorlabs</td>
			<td>WPH05M-1064</td>
			<td></td>
		</tr>
		<tr>
			<td>Triton X-100</td>
			<td>Sigma-Aldrich</td>
			<td>T8787</td>
			<td></td>
		</tr>
		<tr>
			<td>Distilled water</td>
			<td>Invitrogen</td>
			<td>10977-023</td>
			<td></td>
		</tr>
		<tr>
			<td>Si Wafer</td>
			<td>Silicon Quest International</td>
			<td>708069</td>
			<td></td>
		</tr>
		<tr>
			<td>Optical lenses</td>
			<td>Thorlabs</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

fabrication and operation of a nano-optical conveyor belt

使用聚焦激光束捕获并施加力的小颗粒的技术已使纳米生物和物理科学许多关键性的发现，在过去的几十年。在这一领域所取得的进展的邀请甚至较小的系统，并在更大的规模进一步的研究中，有可能更容易地分布并使其更广泛使用的工具。不幸的是，衍射的基本定律限制的激光束，这使得颗粒比半波长更小的直径难以捕集的焦斑的最小尺寸并且通常阻止操作者从颗粒之间鉴别它们是更靠近在一起超过一个半1/4波长。这就排除许多紧密排列的纳米粒子的光学操纵，并限制光学机械系统的​​分辨率。此外，操纵使用聚焦光束需要的波束形成或转向光学器件，它可以是非常笨重和昂贵。讲话在常规的光学捕获我们实验室的系统的可扩展这些限制已经设计出一种替代技术，其利用近场光学器件以跨越一个芯片移动的颗粒。代替聚焦激光束在远场的，电浆谐振器的光学近场产生必要的局部光强度增强，以克服衍射的限制，并在更高分辨率操作的颗粒。紧密间隔的谐振器产生可以加以处理以介导从一个越区切换粒子到下一个传送带状方式强烈光阱。在这里，我们将介绍如何设计和使用金表面图案与等离子C形谐振器和如何与偏振激光来实现超分辨率纳米操纵和运输操作它产生的传送带。纳米光输送带芯片可以使用光刻技术来制造，并容易包装和分发。

Figure 7 is a picture of the final device. At the center of the 1 cm x 1 cm gold surface is the matrix of CSE and conveyor patterns, which can be barely seen from an angled view. Figure 6 is a scanning electron microscopy image of an example CSE pattern on the final device.
The particle motion of a 390 nm polystyrene bead traveling across a nano-optical conveyor belt 5 &#181;m in length is shown in Figure 9. The curve shows the particle&#8217;...

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Fabrication and Operation of a Nano-Optical Conveyor Belt

The scalability and resolution of conventional optical manipulation techniques are limited by diffraction. We circumvent the diffraction limit and describe a method of optically transporting nanoparticles across a chip using a gold surface patterned with a path of closely spaced C-shaped plasmonic resonators.

一个纳米光输送带的制作和操作

The technique of using focused laser beams to trap and exert forces on small particles has enabled many pivotal discoveries in the nanoscale biological ...

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11.5K 次观看.  Stanford University. The scalability and resolution of conventional optical manipulation techniques are limited by diffraction. We circumvent the diffraction limit and describe a method of optically transporting nanoparticles across a chip using a gold surface patterned with a path of closely spaced C-shaped plasmonic resonators.

视频: Fabrication and Operation of a Nano-Optical Conveyor Belt

Fabrication, Densification, and Replica Molding of 3D Carbon Nanotube Microstructures

The introduction of new materials and processes to microfabrication has, in large part, enabled many important advances in microsystems, lab-on-a-chip devices, and their applications. In particular, capabilities for cost-effective fabrication of polymer microstructures were transformed by the advent of soft lithography and other micromolding techniques 1, 2, and this led a revolution in applications of microfabrication to biomedical engineering and biology. Nevertheless, it remains challenging to fabricate microstructures with well-defined nanoscale surface textures, and to fabricate arbitrary 3D shapes at the micro-scale. Robustness of master molds and maintenance of shape integrity is especially important to achieve high fidelity replication of complex structures and preserving their nanoscale surface texture. The combination of hierarchical textures, and heterogeneous shapes, is a profound challenge to existing microfabrication methods that largely rely upon top-down etching using fixed mask templates. On the other hand, the bottom-up synthesis of nanostructures such as nanotubes and nanowires can offer new capabilities to microfabrication, in particular by taking advantage of the collective self-organization of nanostructures, and local control of their growth behavior with respect to microfabricated patterns. 
Our goal is to introduce vertically aligned carbon nanotubes (CNTs), which we refer to as CNT "forests", as a new microfabrication material. We present details of a suite of related methods recently developed by our group: fabrication of CNT forest microstructures by thermal CVD from lithographically patterned catalyst thin films; self-directed elastocapillary densification of CNT microstructures; and replica molding of polymer microstructures using CNT composite master molds. In particular, our work shows that self-directed capillary densification ("capillary forming"), which is performed by condensation of a solvent onto the substrate with CNT microstructures, significantly increases the packing density of CNTs. This process enables directed transformation of vertical CNT microstructures into straight, inclined, and twisted shapes, which have robust mechanical properties exceeding those of typical microfabrication polymers. This in turn enables formation of nanocomposite CNT master molds by capillary-driven infiltration of polymers. The replica structures exhibit the anisotropic nanoscale texture of the aligned CNTs, and can have walls with sub-micron thickness and aspect ratios exceeding 50:1. Integration of CNT microstructures in fabrication offers further opportunity to exploit the electrical and thermal properties of CNTs, and diverse capabilities for chemical and biochemical functionalization 3.

We present methods for fabrication of patterned microstructures of vertically aligned carbon nanotubes (CNTs), and their use as master molds for production of polymer microstructures with organized nanoscale surface texture. The CNT forests are densified by condensation of solvent onto the substrate, which significantly increases their packing density and enables self-directed formation of 3D shapes.

制备，致密，副本三维碳纳米管微结构成型

The introduction of new materials and processes to microfabrication has, in large part, enabled many important advances in microsystems, lab-on-a-chip ...

科研

工程学

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

This paper discusses fluorescent core microcavity-based sensors that can operate in a microfluidic analysis setup. These structures are based on the formation of a fluorescent quantum-dot (QD) coating on the channel surface of a conventional microcapillary. Silicon QDs are especially attractive for this application, owing in part to their negligible toxicity compared to the II-VI and II-VI compound QDs, which are legislatively controlled substances in many countries. While the ensemble emission spectrum is broad and featureless, an Si-QD film on the channel wall of a capillary features a set of sharp, narrow peaks in the fluorescence spectrum, corresponding to the electromagnetic resonances for light trapped within the film. The peak wavelength of these resonances is sensitive to the external medium, thus permitting the device to function as a refractometric sensor in which the QDs never come into physical contact with the analyte. The experimental methods associated with the fabrication of the fluorescent-core microcapillaries are discussed in detail, as well as the analysis methods. Finally, a comparison is made between these structures and the more widely investigated liquid-core optical ring resonators, in terms of microfluidic sensing capabilities.

Fluorescent-core microcavity sensors employ a high-index quantum-dot coating in the channel of silica microcapillaries. Changes in the refractive index of fluids pumped into the capillary channel cause shifts in the microcavity fluorescence spectrum that can be used to analyze the channel medium.

折光传感荧光芯微腔的合成和操作

This paper discusses  fluorescent core microcavity-based sensors that can operate in a microfluidic  analysis setup. These structures are based on the ...

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Surface acoustic waves (SAWs) can be used to drive liquids in portable microfluidic chips via the acoustic counterflow phenomenon. In this video we present the fabrication protocol for a multilayered SAW acoustic counterflow device. The device is fabricated starting from a lithium niobate (LN) substrate onto which two interdigital transducers (IDTs) and appropriate markers are patterned. A polydimethylsiloxane (PDMS) channel cast on an SU8 master mold is finally bonded on the patterned substrate. Following the fabrication procedure, we show the techniques that allow the characterization and operation of the acoustic counterflow device in order to pump fluids through the PDMS channel grid. We finally present the procedure to visualize liquid flow in the channels. The protocol is used to show on-chip fluid pumping under different flow regimes such as laminar flow and more complicated dynamics characterized by vortices and particle accumulation domains.

In this video we first describe fabrication and operation procedures of a surface acoustic wave (SAW) acoustic counterflow device. We then demonstrate an experimental setup that allows for both qualitative flow visualization and quantitative analysis of complex flows within the SAW pumping device.

制造，操作和表面声波驱动声逆流微流体流动可视化

Surface acoustic waves (SAWs) can be used to drive liquids in portable microfluidic chips via the acoustic counterflow phenomenon. In this video we ...

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Cavity optomechanics experiments that parametrically couple the phonon modes and photon modes have been investigated in various optical systems including microresonators. However, because of the increased acoustic radiative losses during direct liquid immersion of optomechanical devices, almost all published optomechanical experiments have been performed in solid phase. This paper discusses a recently introduced hollow microfluidic optomechanical resonator. Detailed methodology is provided to fabricate these ultra-high-Q microfluidic resonators, perform optomechanical testing, and measure radiation pressure-driven breathing mode and SBS-driven whispering gallery mode parametric vibrations. By confining liquids inside the capillary resonator, high mechanical- and optical- quality factors are simultaneously maintained.

Parametric optomechanical excitations have recently been experimentally demonstrated in microfluidic optomechanical resonators by means of optical radiation pressure and stimulated Brillouin scattering. This paper describes the fabrication of these microfluidic resonators along with methodologies for generating and verifying optomechanical oscillations.

制造和微流控光机振荡器测试

Cavity optomechanics experiments that parametrically couple the phonon modes and photon modes have been investigated in various optical systems including ...

Fabrication Process of Silicone-based Dielectric Elastomer Actuators

This contribution demonstrates the fabrication process of dielectric elastomer transducers (DETs). DETs are stretchable capacitors consisting of an elastomeric dielectric membrane sandwiched between two compliant electrodes. The large actuation strains of these transducers when used as actuators (over 300% area strain) and their soft and compliant nature has been exploited for a wide range of applications, including electrically tunable optics, haptic feedback devices, wave-energy harvesting, deformable cell-culture devices, compliant grippers, and propulsion of a bio-inspired fish-like airship. In most cases, DETs are made with a commercial proprietary acrylic elastomer and with hand-applied electrodes of carbon powder or carbon grease. This combination leads to non-reproducible and slow actuators exhibiting viscoelastic creep and a short lifetime. We present here a complete process flow for the reproducible fabrication of DETs based on thin elastomeric silicone films, including casting of thin silicone membranes, membrane release and prestretching, patterning of robust compliant electrodes, assembly and testing. The membranes are cast on flexible polyethylene terephthalate (PET) substrates coated with a water-soluble sacrificial layer for ease of release. The electrodes consist of carbon black particles dispersed into a silicone matrix and patterned using a stamping technique, which leads to precisely-defined compliant electrodes that present a high adhesion to the dielectric membrane on which they are applied.

This manuscript shows the fabrication process for the manufacture of dielectric elastomer soft actuators based on silicone membranes. The three key stages of production are presented in detail: blade casting of thin silicone membranes; pad printing of compliant electrodes; and the assembly of all the components.

有机硅为基础的介电弹性体致动器的制作工艺

This contribution demonstrates the fabrication process of dielectric elastomer transducers (DETs). DETs are stretchable capacitors consisting of an ...

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must be smaller than the incident wavelength of sound and the width of the fluidic channels are typically tens to hundreds of micrometers across, acoustofluidic devices typically use ultrasonic waves generated from a piezoelectric transducer pulsating at high frequencies (in the megahertz range). At characteristic frequencies that depend on the geometry of the device, it is possible to induce the formation of standing waves that can focus particles along desired fluidic streamlines within a bulk flow. Here, we describe a method for the fabrication of acoustophoretic devices from common materials and clean room equipment. We show representative results for the focusing of particles with positive or negative acoustic contrast factors, which move towards the pressure nodes or antinodes of the standing waves, respectively. These devices offer enormous practical utility for precisely positioning large numbers of microscopic entities (e.g., cells) in stationary or flowing fluids for applications ranging from cytometry to assembly.

Acoustofluidic devices use ultrasonic waves within microfluidic channels to manipulate, concentrate and isolate suspended micro and nanoscopic entities. This protocol describes the fabrication and operation of such a device supporting bulk acoustic standing waves to focus particles in a central streamline without the aid of sheath fluids.

制造和Acoustofluidic设备的运营支撑体声波驻波的聚焦鞘颗粒

Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must ...

Fabrication and Characterization of Superconducting Resonators

Superconducting microwave resonators are of interest for a wide range of applications, including for their use as microwave kinetic inductance detectors (MKIDs) for the detection of faint astrophysical signatures, as well as for quantum computing applications and materials characterization. In this paper, procedures are presented for the fabrication and characterization of thin-film superconducting microwave resonators. The fabrication methodology allows for the realization of superconducting transmission-line resonators with features on both sides of an atomically smooth single-crystal silicon dielectric. This work describes the procedure for the installation of resonator devices into a cryogenic microwave testbed and for cool-down below the superconducting transition temperature. The set-up of the cryogenic microwave testbed allows one to do careful measurements of the complex microwave transmission of these resonator devices, enabling the extraction of the properties of the superconducting lines and dielectric substrate (e.g., internal quality factors, loss and kinetic inductance fractions), which are important for device design and performance.

Superconducting microwave resonators are of interest for detection of light, quantum computing applications and materials characterization. This work presents a detailed procedure for fabrication and characterization of superconducting microwave resonator scattering parameters.

制备与表征超导谐振器的

Superconducting microwave resonators are of interest for a wide range of applications, including for their use as microwave kinetic inductance detectors ...

Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices

Thermoplastic microfluidic devices offer many advantages over those made from silicone elastomers, but bonding procedures must be developed for each thermoplastic of interest. Solvent bonding is a simple and versatile method that can be used to fabricate devices from a variety of plastics. An appropriate solvent is added between two device layers to be bonded, and heat and pressure are applied to the device to facilitate the bonding. By using an appropriate combination of solvent, plastic, heat, and pressure, the device can be sealed with a high quality bond, characterized as having high bond coverage, bond strength, optical clarity, durability over time, and low deformation or damage to microfeature geometry. We describe the procedure for bonding devices made from two popular thermoplastics, poly(methyl-methacrylate) (PMMA), and cyclo-olefin polymer (COP), as well as a variety of methods to characterize the quality of the resulting bonds, and strategies to troubleshoot low quality bonds. These methods can be used to develop new solvent bonding protocols for other plastic-solvent systems.

Solvent bonding is a simple and versatile method for fabricating thermoplastic microfluidic devices with high quality bonds. We describe a protocol to achieve strong, optically clear bonds in PMMA and COP microfluidic devices that preserve microfeature details, by a judicious combination of pressure, temperature, an appropriate solvent, and device geometry.

溶剂粘接的PMMA的制备和COP微流体装置

Thermoplastic microfluidic devices offer many advantages over those made from silicone elastomers, but bonding procedures must be developed for each ...

Construction and Operation of a Light-driven Gold Nanorod Rotary Motor System

The possibility to generate and measure rotation and torque at the nanoscale is of fundamental interest to the study and application of biological and artificial nanomotors and may provide new routes towards single cell analysis, studies of non-equilibrium thermodynamics, and mechanical actuation of nanoscale systems. A facile way to drive rotation is to use focused circularly polarized laser light in optical tweezers. Using this approach, metallic nanoparticles can be operated as highly efficient scattering-driven rotary motors spinning at unprecedented rotation frequencies in water.
In this protocol, we outline the construction and operation of circularly-polarized optical tweezers for nanoparticle rotation and describe the instrumentation needed for recording the Brownian dynamics and Rayleigh scattering of the trapped particle. The rotational motion and the scattering spectra provides independent information on the properties of the nanoparticle and its immediate environment. The experimental platform has proven useful as a nanoscopic gauge of viscosity and local temperature, for tracking morphological changes of nanorods and molecular coatings, and as a transducer and probe of photothermal and thermodynamic processes.

Plasmonic gold nanorods can be trapped in liquids and rotated at kHz frequencies using circularly-polarized optical tweezers. Introducing tools for Brownian dynamics analysis and light scatteringspectroscopy leads to a powerful system for research and application in numerous fields of science.

轻型金纳米旋转电机系统的施工与运行

The possibility to generate and measure rotation and torque at the nanoscale is of fundamental interest to the study and application of biological and ...

Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics

The use of microfluidic devices has emerged as a defining tool for biomedical applications. When combined with modern microscopy techniques, these devices can be implemented as part of a robust platform capable of making simultaneous complementary measurements. The primary challenge created by the combination of these two techniques is the mismatch in refractive index between the materials traditionally used to make microfluidic devices and the aqueous solutions typically used in biomedicine. This mismatch can create optical artifacts near the channel or device edges. One solution is to reduce the refractive index of the material used to fabricate the device by using a fluorinated polymer such as MY133-V2000 whose refractive index is similar to that of water (n = 1.33). Here, the construction of a microfluidic device made out of MY133-V2000 using soft lithography techniques is demonstrated, using O2 plasma in conjunction with an acrylic holder to increase the adhesion between the MY133-V2000 fabricated device and the polydimethylsiloxane (PDMS) substrate. The device is then tested by incubating it filled with cell culture media for 24 h to demonstrate the ability of the device to maintain cell culture conditions during the course of a typical imaging experiment. Finally, quantitative phase microscopy (QPM) is used to measure the distribution of mass within the live adherent cells in the microchannel. This way, the increased precision, enabled by fabricating the device from a low index of refraction polymer such as MY133-V2000 in lieu of traditional soft lithography materials such as PDMS, is demonstrated. Overall, this approach for fabricating microfluidic devices can be readily integrated into existing soft lithography workflows in order to reduce optical artifacts and increase measurement precision.

This protocol describes the fabrication of microfluidic devices from MY133-V2000 to eliminate artifacts that often arise in microchannels due to the mismatching refractive indices between microchannel structures and an aqueous solution. This protocol uses an acrylic holder to compress the encapsulated device, improving adhesion both chemically and mechanically.

生物医学微流体折射率匹配器件的研制

The use of microfluidic devices has emerged as a defining tool for biomedical applications. When combined with modern microscopy techniques, these devices ...