Name: measuring cardiac autonomic nervous system (ans) activity in toddlers - resting and developmental challenges
Uploaded: 2016-02-25T14:00:00.000+00:00
Duration: 8 min 22 s
Description: Watch this Scientific Journal Video about Measuring Cardiac Autonomic Nervous System ANS Activity in Toddlers - Resting and Developmental Challenges at JoVE.com

This&#160;research was supported by NIH 1 U01 HL097973, NHLBI 5 R01 HL116511-02, UCSF-CTSI Grant Number UL1 TR000004, the Robert Wood Johnson Health and Society Scholars Program, and the Lisa and John Pritzker Family Foundation. The authors also wish to acknowledge Michelle Stephens for her assistance with scoring the ANS data as well as Vanessa Tearnan, Marialma Gonzales-Cruz, Yurivia Cervantes, Amy Engler, Stephanie Grover and Karen Jones-Mason for their assistance in collecting the ANS data, and Michael Coccia for his help with the data. We are also thankful to the families for their generous participation in this research and to the volunteers who helped us to test and refine this protocol, including the first author&#8217;s two children. We want to acknowledge our mentors who taught us about measuring ANS and why measuring ANS responsivity is meaningful in young children: W. Thomas Boyce, Gary Bernston, and Dave Lozano.

这项研究是经委员会加州大学旧金山分校的人类研究。 1.预协议建立（参见设备的完整列表材料的电子表格） <ol><li>通过打开计算机和连接ANS数据采集单元到电源安装设备。 注意:不要把采集单元上。 </li><li>在此之前开始任何数据采集，建立了ANS数据采集软件。选择设置，以符合图1。 </li></ol><img alt="图1" src="/files/ftp_upload/53652/53652fig1.jpg" /> 图1. BIOLAB软件配置设置。如在步骤1.2中描述此图显示的ANS数据收集期间将要使用的配置设置的屏幕截图。 <ol start="3"><li>现货保持电极在一个密封的包装，直至需要它们，因为电极质量下降了时间暴露于空气中。抓拍点电极到每个使用硬表面7捕捉线索。确保引线解开并牢固地连接到电极上。 </li><li>打开和提示音频和视觉应用（见下文步骤4）的片剂。设置的音量，以在片剂和全卷上的扬声器最大体积的80％。 </li><li>设置在不显眼的地方三脚架和摄像机。 </li><li>事先设置柠檬汁任务物品开始​​的协议。倒入一小纸杯一些水柠檬汁任务（约3盎司）之后冲洗。把柠檬汁极少量从分组到另一个杯和柠檬汁2滴（该量将达到的移液管的第一缩小和等于约0.1毫升）填充一次性塑料吸管。在纸巾或柠檬汁杯休息滴管，并设置出的方式。 </li></ol> 2.准备的发展挑战协议的母亲和儿童<ol><li>确保两个人，儿童评估机构（CA）和电脑操作员（CO），准备以管理协议。引进人员的母亲和孩子，让他们舒服。眼神接触，跟我们的孩子，以建立母，子，和员工之间的信任关系。让孩子一定的空间，使其恢复到CA和CO。不要关注压倒了孩子。 </li><li>问母如果孩子需要被馈送或具有电极置于孩子之前以避免以后中断他或她的尿布改变。让母亲开始之前的协议顾不上孩子的需求。 </li><li>让孩子在地板上或引进过程中一个开放的空间发挥。一旦协议开始，指导母亲让孩子在她的腿上，直到它完成。 </li><li>通过阅读剧本审查与母亲协议（见补充信息）。此外，发言的孩子直接左右了我回事访问期间发生。 </li></ol> 3.连接ANS采集设备儿童<ol><li>指示施加在电极对孩子的颈部和躯干（ 见图2）在CA，而CO分散了孩子。使用白色圆圈贴纸展示一个娃娃电极位置，如果有必要搞的孩子。快速移动和放置电极孩子的衬衫下面，以尽量减少可能性孩子将触摸或移开电极。 </li></ol><img alt="图2" src="/files/ftp_upload/53652/53652fig2.jpg" /> 图2.电极配置18个月孩子注 :2 ECG电极在铅II配置应用。此外，对于颈背替代电极放置在此显示。 <ol start="2"><li>直接应用在电极到皮肤上。按用手指在纸带凝胶周围，以确保Ť帽子当场电极附着到皮肤上。所有电极必须从邻近的电极，以确保正确的信号传导被置于相距至少3厘米。对于那些难以确定任何距离，应用卷尺测量和通过测量每个电极的中心之间的长度验证此最小距离。 注意:将电极对儿童的下列顺序一直有效，以幼儿良好的耐受性。 <ol><li>放置连接到红色的电极（ - ）上的下部的中心引回。 </li><li>放置附着在白色电极（ - ）导致正上方的剑突，它位于xiphosternal结（胸骨底部尖端;其中所述左和右肋骨相遇）。确保该电极是红上述至少3个厘米（ - ）点电极。 </li><li>放置连接到刚刚离开的锁骨的边缘胸骨上切迹（评估的右侧）的白色（+）导线的电极。 <ol><li>使用卷尺测量，验证并记录在厘米2前电极之间的距离（（白色（+）和白 - ）），用于在阻抗得分程序以后使用。 </li></ol></li><li>将连接到棕色第一ECG电极（ - ）铅对右锁骨那里的锁骨符合肩膀骨。按下电极周围的泡沫和感觉锁骨。将电极沿纵向骨。 </li><li>放置连接至左下肋棕色（+），引线的第二ECG电极。按在电极周围的泡沫和感觉肋骨。 </li><li>放置连接到黑色的接地电极（ - ）铅对儿童的右下肋符合左下肋电极的水平面。 </li></ol></li><li>包和剪辑导致孩子的衣服下端，以尽量减少引线的运动。指示CO监控计算机。指示CA从事儿童和审查的Activiti参与与母亲的协议ES（见脚本B中的补充信息）。 </li><li>为了获得阻抗和心电图（ECG）信号转ANS数据获取单元上。按"重新检测"，开始心电图分析，然后单击一次"开始"旁边的绿灯在程序窗口的右下角。 <ol><li>等待5分钟后，在电极被放置以允许凝胶充分粘附到孩子的皮肤对所述ECG和阻抗信号的最佳电导。 5分钟后，检查电脑屏幕，查看信号，并确保他们看起来正常（CO）。转动摄像机（CO）上。 <ol><li>避免在这段时间与孩子闲聊。报价孩子举行一个标准化的"可选"的毛绒玩具，如果他或她（以协议开始计划从手工删除玩具前夕），似乎不舒服。 注:解释给孩子和母亲，这是需要等待一个双向吨至确保设备工作正常。 </li><li>显示孩子的心脏活动，母亲的计算机图像并解释每个信号代表。提醒母亲，这一活动是不用于诊断任何健康问题。向她保证，如果研究小组注意到令人关注的东西，她会收到通知。按照脚本B（见补充信息）。 </li></ol></li></ol></li></ol> 4.管理任务的发展挑战议定书<ol><li>打开片剂媒体播放器。播放第1分钟的摇篮曲。保持平板电脑出孩子够不到的地方。 </li><li>持有玩偶的盒子在孩子面前。慢慢的旋转手臂，播放音乐，直到袜子猴子弹出，使得它需要15秒才能完成一个回合。重复四次。 </li><li>开始与脚本C柠檬汁任务（见补充信息）。将2滴浓柠檬汁对孩子的舌头的中心，稍有BAC从前面K，所以果汁不顺口。 注:如果吸管在果汁杯休息，抖落之前放弃它在舌头上的吸管外额外滴。 <ol><li>静静地等待10秒，一个友好的，中性的表情。说:"什么味道？"如果孩子需要安慰。 注:柠檬汁任务必须至少需要20秒，从通过等到后滴在他们口中，然后看是否有反应讲述液体孩子开始。 </li><li>滴柠檬汁后至少10秒说，"现在是时候喝一些水"，并给孩子喝了一口水。如果孩子拒绝，等待几秒钟，说，再试试"现在你可以拿点水喝。"按F4键作为评估开始提供水（CO）。 </li></ol></li><li>玩患病婴儿使用媒体平板电脑上哭30秒记录播放器。保持平板电脑出孩子够不到的地方。 </li><li>播放使用媒体播放器平板电脑的第二个1分钟的摇篮曲。保持平板电脑出孩子够不到的地方。 </li><li>播放使用媒体播放器平板电脑的2分钟视频中性。握住平板电脑的孩子的脸大致12英寸，但不要让他们抓住不放的平板电脑。 </li></ol> 5.标记协议段在发展方面的挑战管理局（CO） <ol><li>指示CO推功能（F）键（由用户设定的先验数据评分期间确定的任务或潜在的问题），以纪念在ANS数据中的重要步骤协议（ 见表1）。按下F11键表示一个任务提前结束（键入"结束任务早"，并解释到文本框）或重大协议问题或违法行为，如电极脱落或有人在房间里走动和分散注意力的孩子（类型解释到文本框中）。 </li></ol><table><tbody><tr><td> Marker键 </td><td> 事件 </td><td> 持续时间后开始 </td></tr><tr><td> F1 </td><td>摇篮曲1日开始</td><td> 60秒</td></tr><tr><td> F2 </td><td>杰克在框中启动</td><td> 60秒</td></tr><tr><td> F3 </td><td>柠檬汁脚本启动</td><td>至少20秒</td></tr><tr><td> F4 </td><td>柠檬汁端（水喝提供） </td><td>至少有10个SES </td></tr><tr><td> F5 </td><td>婴儿哭声的开始</td><td> 30秒</td></tr><tr><td> F6 </td><td>摇篮曲2启动</td><td> 60秒</td></tr><tr><td> F7 </td><td>视频开始</td><td> 120秒</td></tr><tr><td> F10 </td><td> DCP结束（视频终端） </td><td rowspan="2"></td></tr><tr><td> F11 </td><td>严重的不规则事件</td></tr></tbody></table> 表1:分子标记键协议事件标志期间挑战的任务。如在步骤5.1中所述标记的键被显示，用于指示在ANS数据文件重要协议事件和很少发生。 6.邮政发展挑战协议<ol><li>有CA对母亲说:"那么，如何是你 （暂停听到回应） 这是有趣的，看看孩子对这些各种各样的活动如何应对？现在是时候要轻轻地从孩子的皮肤去除电极"。 </li><li>感人/取出电极之前先关闭ANS的数据采集。 注:该设备将无法进行，或当它被关闭传输电​​力。 </li><li>通过使用一个手指紧握皮肤，同时另一方面消除电极，除去从孩子电极。拉起电极，并从标签指向的方向走的标签。除去最敏感电极最后一个（前部和颈部的背面）。让母亲重新移动电极如果孩子特别不安或者如果她期望。用湿擦拭以去除来自电极的皮肤和干纱布垫剩余如果皮肤潮湿或有凝胶在皮肤上的任何多余的残留物。 </li><li>关闭摄像机。保存ANS数据文件，退出程序，并关闭计算机。 注:ANS数据采集软件创建两个数据文件，".mw"文件（原始ANS数据）和一个".event"文件，它是按下的功能键的文本文件。 </li></ol> 7.呼吸性窦性心律心律失常评分<ol><li>打开心率变异性（HRV）分析方案，以清洁的心电数据和得分率心脏（HR），RSA和呼吸频率（RR）。 </li></ol><img alt="图3" src="/files/ftp_upload/53652/53652fig3.jpg" /> 图3. HRV分析主导航画面。为计分用的HRV蹒跚学步ANS数据设置alysis记分软件显示出来。得分段，拖动事件到"事件类型"框中。 <ol start="2"><li>查看数据收集任何可用的现场记录，以了解可能发生的异常情况或错误。找到并双击从弹出的窗口中攻入".mw"文件（"选择或输入文件路径"）。 注:被砍下应该出现在白框在屏幕顶部的参与者数据的文件名。 <ol><li>将"通道标签"如下:1通道心电图，通道2 - 莫宁，通道3 DZ / DT。验证该采样频率被设置为1,000。点击"确定"，在屏幕的底部打开主导航画面（ 图3）。 注意:这些通道应与数据收集过程中设置的。 </li><li>选择设置以匹配的身影。将模式设置为"事件"。设置事件标识符为"名"查看任务的名称（ 即 "玩偶的盒子"）。将"邮事件时间（s）"，以30秒。 注意:文件长度为每个孩子的文件有所不同。 "类型"是指由CO协议期间按功能（F）键。如果模式是不是事件，而是时间，"细分时代"，可以在几秒钟内（ 即 30设置为不同的时间长度或60）。 </li><li>点击"HRV校准设置"标签。一套计算方法，"整个"。呼吸信号设置为"咗"（DZ不是/ DT）。设置窗口函数为"海明"。设置VLF频带范围为0.003至0.04赫兹（Hz），低频或LF波段范围0.04到0.15赫兹，和高频HF频带范围0.15-1.04赫兹婴幼儿。 </li><li>点击下一个标签，"R＆山顶神器设置"设置IBI和MAD MED为"on"。设置最大心率（每分钟心跳（BPM））230和最小心率40 BPM。对于R峰值检测灵敏度滑块设置为1。设置手动操作来关闭。打开基线和肌肉噪声滤波器（亮绿色）和陷波滤波器关闭（深灰色）。噪声滤波器设置为60 Hz。 </li><li>点击下一个标签，"其他设置"。点击是/输出设置部分没有框（"使用默认输出路径"）来选择默认位置来保存输出文件。勾选"显示报告"按钮（亮绿色）。 注:同步的视频可以通过点击"是/否"框下被视为一个以前编辑过的数据文件可以通过单击"使用编辑的数据"是/否盒被视为"使用默认的视频路径。" </li><li>回到"事件和模式"​​选项卡。点击"分析"按钮。 </li><li>选择"是"或"否"当记者问，"难道心电图反转？否/是"。 注:多数心电图不反转。分析窗口将打开，显示数据的30秒。该HRV分析程序，标志着淡蓝色圆点每个R峰值时有一个潜在的"异常值"或不规则图案，标志着一个黄色的星星。离群值可以被标记时两个R峰之间的距离是不同的，为了这个个体经常RR间隔。 </li><li>检查所有的异常值（黄星），以确定黄星的原因（见7.1.1.9编辑选项）。在数据记录的利息登录的变量进行任何必要的编辑之后。 注:分析窗口右下角的框显示由这一细分市场，包括HR，RR和RSA的计算值。 </li><li>点击"编辑R"原则（在控制窗口中的绿色按钮）来检查异常值。点击放大镜图标，以进一步放大]。 </li><li>使用红色的"删除"按钮来删除黄色的星星被放置不正确和白色的"插入"按钮为纪念第r峰。 注:通常是清楚其中R峰值应删除或手动标记。极杂乱部分可以使用黄色"删除数据部分"按钮被移除，左右滑动的部分中的浅蓝色X游标之后被删除。这应该只在开头或分段的端部进行;必须有数据来计算一个30秒段的有效分数的连续20秒。 </li><li>点击"确定"在屏幕底部的保存编辑，返回到先前的屏幕，显示编辑的数据。点击"写入"（顶部工具栏中的白色按钮）来输出数据打进到Excel文件。重复此过程，每30秒间隔或任务进行评分。 注:"取消"退出而不保存更改。 </li><li>点击"完成"的"控制"面板上的编辑和写入数据的全部30秒后段。 注:弹出窗口将请求的文件名，编辑DAT一个。所有编辑的数据，并打进了每个孩子的数据将被保存一个.edh文件。 </li></ol></li></ol> 8.预射血期评分注意:阻抗（IMP）分析程序允许一个清洁阻抗数据，并获得人力资源和PEP值。 <img alt="图4" src="/files/ftp_upload/53652/53652fig4.jpg" /> 图4.进出口分析阻抗校准设置屏幕为计分采用阻抗分析软件得分蹒跚学步ANS数据的设定显示。 <ol><li>打开IMP分析程序。在IMP分析程序双击，然后选择刚刚打进HRV分析同一.mw文件。选择每个相同的渠道"频道标签:"通道1心电图，通道2 - 莫宁，通道3 DZ / DT。按"OK。"关于事件和模式选项卡中，选择相同的设置作为HRV分析程序。 <ol><li>点击"ImpedanCE校准设置"选项卡（ 图4）。选择设置以匹配图。设置"电极式"为"点"。 （所测量和数据收集期间记录白色电极之间的距离）进入电极的距离。设置"合奏长度（毫秒）"，以400为幼儿。 </li><li>设置的"Q点的计算方法"，以"最小值KR间隔"。对于18个月年轻人（K值可以基于每个子基，如果需要进行调节）设置K值到25。设置的"B点的计算方法"，以"的DZ / dt的时间+ C百分数"设置的百分比DZ / dt的峰值为"55"，而常数"4"。将"DZ / dt的来源"为"测量"。验证"Detrend DZ / dt的"灯亮了非移动采集单元。 </li><li>点击"R＆山顶神器设置"标签。验证IBI最小/最大和MAD MED检查都"在"设置最大心率（BPM），以230为幼儿和最小心率（BPM）为40设置峰值检测灵敏度1.将手动覆盖到了。打开"基线和肌肉噪声滤波器"（亮绿色）。关闭"陷波滤波器"（深绿色）。关闭60 Hz的设置。 </li><li>点击"更多设置"标签。不要选中"自动分析所有数据段"按钮。选择"是"，在"使用编辑的数据"是/否框，如果任何编辑都在HRV程序进行。双击从弹出框的编辑（.edh）文件。选择"创先争优"为输出模式。点击明亮的绿色"分析（回车）"按钮。 注:如果在得分HRV没有编辑被做了，这是没有必要（或可能）在这里使用编辑数据。 </li><li>点击"编辑合奏"看到合奏波，这是图像的全屏视图的平均值所有波形从段。 </li><li>检查Q点，这是通过在设置中输入K值确定的位置。通过所有段的滚动检查在Q位置。 注:Q点应该只是之前的红色（EKG）线开始上升到R峰值的最低点。如果有一个逐渐倾斜"预Q"区，则Q应在点被标记，其中斜率至R开始更壁立。 </li><li>返回阻抗校准设置选项卡，如果Q点盯人不正确的，当K = 25增加5 K值。再次回顾段。继续更改到K，直到在Q标志是最优的。 注:K值需要为每个孩子保持一致;没有针对不同的细分选择不同的价值观，不要用手移动任何标记。 </li><li>记录PEP值，每个段得分。 注:PEP是每一个人在相当稳定，一般由4-6毫秒整个评估的波动每个孩子。然而，PEP值可以是在整个儿童非常不同。 </li><li>为了输出的得分数据点击"写入"按钮，Excel文件。重复这个过程被打进每个部分。 </li></ol></li></ol>

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G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chapter+20,+Developmental+Psychophysiology:+Conceptual+and+Methodological+Issues.">Chapter 20, Developmental Psychophysiology: Conceptual and Methodological Issues.</a> Handbook of Psychophysiology. , 453-481 (2007).</li><li>Gilissen, R., Koolstra, C., van Ijzendoorn, M., Bakermans-Kranenburg, M., van der Veer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physiological+reactions+of+preschoolers+to+fear-inducing+film+clips:+Effects+of+temperamental+fearfulness+and+quality+of+the+parent-child+relationship.">Physiological reactions of preschoolers to fear-inducing film clips: Effects of temperamental fearfulness and quality of the parent-child relationship.</a> Dev. Psychobiol. 49, 187-195 (2007).</li><li>Bush, N. 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The authors declare that they have no competing financial interests.

这项研究表明，18个月大的孩子，标准化的休息和挑战任务，旨在从一系列域（惊吓，感官，社交，休息）的得到响应样品中导致了一系列的ANS响应。这些休息和挑战的条件下发现，平均ANS反应是类似于谁已经测量ANS的反应与使用类似的任务8,11,27,29幼儿人报道ANS值。在我们的样本中发现的ANS措施的平均水平是那些通常在婴幼儿发现和那些在年龄较大的儿童中发现，这是符合证据之间的心脏率下降和RSA和儿童在10岁时与PEP增加。我们还发现有一个范围横跨三个生理指标的反应得分（从静止状态到挑战条件下的生理反应的差异），与众多的之间的多个统计显著有意义的差异挑战和休息得分RSA和PEP。总的来说，这些研究结果表明，该协议是成功的在平静的活动和发育有挑战性的任务之间的转换过程中捕获ANS变化，引发在充分研究的年龄组都ANS的交感神经和副交感神经分支中的一个可衡量的应激反应。除了为在ANS样品级平均变化的证据，个人演示显著变异搁ANS水平，任务ANS响应和挑战的反应性分数，它提供了进一步的证据，该协议是用于在幼儿ANS个体差异检查有用35。 广大婴幼儿ANS研究只包括RSA的ANS的副交感神经分支，由于在这个非常年轻的年龄组评估同情措施的难度。因此，鲜为人知的是，在A交感神经分支的发展在休息或响应NS这一时期的发展或者是什么因素塑造其发展和健康相关的轨迹内挑战。此外，我们可以了解更多关于通过同时测量他们在SNS和PNS之间的动态或同步或异步的关系，所以需要双反措施。从该协议的结果表明，这两个SNS和PNS活动和变化的测量是可能在这个年龄组。 虽然与成人反应性协议通常涉及与"休息"期间，成人5分钟或坐或躺着不动无刺激，休息幼儿措施的时间段是具有挑战性的。在一些最起码的方式从事幼儿有必要限制运动伪影在休息29,36 ANS措施。在以前的研究中，年幼的孩子"休息或基线""被评定为婴儿听摇篮曲8，或学龄前儿童柒德伦读了一个轻松的故事11所示，中性影片剪辑4或视频屏幕改变形状37。的共识是，这些可能构成合理的参考点取决于比较条件评估的反应。从我们的5岁儿童38样品内的实验室先前的实验表明，通过运动抑制基线条件诱发的精神活动（ 例如，说话，社会参与，打手势）是一个重要的考虑因素;研究结果表明，利用社会参与来让孩子冷静和专注，比如读一个故事，是社会的挑战，而中性的影片也可能因反应作为比较值是合适的影片唤起孩子一个适当的基准进行比较。参加到视频与越来越多的关注相关联，并且它具有在ANS的两个分支镇静响应。在这项研究中，为幼儿，中性视频没有引起ðifferences在人力资源，RSA或PEP的措施，相对于第一静止催眠曲，表明要么可能已用作休息值用以计算反应性，或将其列入协议是不必要的。此外，该协议不包括任务之间的休息时间，这可能会导致从任务"结转"的反应效果任务。这个选择，因为在保持宝宝的注意力长时间的困难的。然而，显著任务到任务的变化分值，最常见于相反的方向显示，携带效应可能在这个协议中被限制。 对于这里所描述的评估数据的设置提供了一个可能的限制;大约有一半的评估是在参加家庭完成，其他人在我们的实验室里完成的。这种潜在的限制是由参与者在完成房屋评估的灵活性，这使我们能够平衡保持数据的收集与与会者谁不能或不愿前往我们的实验室。此外，虽然所有的孩子有一个5分钟等待电极放置和发展挑战协议的开始之间的时间，另一种潜在的限制是，有些孩子变得由电极的放置无奈之举，可能已经在协议8开始被强调。因此，我们可能没有在协议开始得到有效的休息措施。这个协议包括为了抵消这种可能性的第二催眠曲，虽然其发生后立即挑战也可能导致交叉污染的效果。评估"真正"的休息条件的限制能力是ANS研究人员幼儿38工作的长期挑战。更一般地，评估幼儿是具有挑战性的，有时与需要的程序（例如，停止换尿布或允许儿童持有他们的SM偶然修改所有的毛绒玩具，以防止发脾气，禁止考核的延续）。在这个协议中，我们设置在啮合和分散幼儿细节，以减少需要这些修改的可能性。开展与幼儿的研究总是需要通过发育敏感人员的灵活性和故障排除。总体而言，开发与参与的母亲的协作关系是重要的。 总之，本研究的结果表明，这里提出的发展挑战协议和ANS数据收集程序可能为研究人员提供了一组引起了广泛的响应横跨ANS的两个分支的儿童年龄18-21个月的结构化的挑战。随着孩子的年龄均与反应性分数相关，这些任务可以适当与年龄稍大的孩子，虽然这不是在这里测试;如果试图，ANS的得分设置将需要调整。该公关otocol提供了难以评估年龄组的学习逆境生理和发展在生命的早期一个选项。每个域计算的反应性可以作为已经与年龄较大的儿童6进行单独检查，或者可以根据反应变量之间的关联在给定的组合以创建一个"整体的反应性"评分作为已与婴儿8完成，样本和研究的目标。从这个协议中收集的数据结果可以比较被年轻8岁的孩子10,38完成类似的年龄适当的任务。 ANS评估在不同年龄段正常允许跨时间发展轨迹的检查和对他们既个体发育和预测的精神和身体健康状况的能力有关问题的追求。

在孩子们的自主神经系统的个体差异（ANS）活性发挥的生理和心理健康问题12-16发展和维护具有重要作用。越来越多的证据体现出，在休息和反应性ANS措施个体差异预测儿童和外向精神病理学4,9,17-19和身体健康7,20的变化。除了​​ANS运作这些直接或主要作用，研究的积累的身体已经发现，孩子们的反应可以用上下文的危险因素，如不良事件或婚姻冲突，中度对儿童的福祉和健康的影响进行交互（见参考文献21复审）。尽管越来越多的证据，儿童的自主反应起到保健作用在整个生命过程中，在这些过程中伴随的兴趣，有是需要更多的研究三岁下检查ANS功能在儿童中的发展。 该ANS包括两个分支，副交感神经和交感神经系统。它调节内脏（如心脏速率，呼吸，消化，性兴奋）的功能，主要是通过无意识的机制，以应对日常和不良反应1。副交感神经系统（PNS）是"休息和恢复"的系统，睡觉或放松交感神经系统（SNS）时保持较低的休息心脏率和恢复状态是响应紧急情况或威胁"战斗或逃跑"系统通过加速一个人的心脏率23的情况。呼吸性窦性心律不齐（RSA）是对心脏功能的PNS影响的可靠指标，和射血前期（PEP）是对汽车在SNS影响的可靠指标DIAC工作。 RSA和PEP已经发现通过在成人24,25的样品的实验药理学阻断有效的措施。最近，这项工作已经扩展到儿童和青少年的样本，进一步确立了RSA和PEP在整个发展8,26,27 ANS活动的有效和可靠的措施。使用带和现场电极可靠心阻抗测量（PEP）已成人样本28被使用相当长的时间。最近，平行测量和分析技术也被证明是可靠的，并在儿童11,29,30的样本有效的，尽管在幼儿的PEP措施集合是罕见的。 有对PNS在婴幼儿，包括迷走神经张力和呼吸性窦性心律不齐措施发挥作用的现存的大量文献。有使用PEP的SNS的研究较少，很少同时检查这两个副交感神经和交感神经系统，这是建立自己的高度集成的功能的理解是至关重要的。幼儿PEP的一些研究中，大多数发现自己的协议不产生响应的挑战8.31的PEP显著集团层面的变化。有研究表明，有稳定搁在婴儿期开始PNS措施，他们与气质，行为和健康，13,16,26,32,33关系，但有SNS的理解有限响应和稳定性随着时间的推移，关系发展，塑造其发展轨迹的因素。 PNS响应已被主要归因于社会参与16和SNS的反应提出了体现"战斗或逃跑反应"1以及奖励灵敏度（见参考文献34复审）。在年幼的孩子，这是具有挑战性的，以模拟在laborato具体的挑战RY和他们搞由于他们的注意力持续时间短。还有谁可能感到不适与同时测量PNS与SNS需要七个点电极测量幼儿的挑战。此外，发展科学领域内，有关于如何激发"应激反应"，同时充分尊重也与儿童工作儿童的发展需求和职业道德标准很少。本文介绍了关于由设计引起从18个月大的孩子和副交感神经交感神经系统的反应休息和挑战性的环境的标准化协议的管理和评分信息。 ANS反应通常是概念化为离散相对于外部刺激的生理反应比较或休息状态，横跨生物个体差异22。的精神和肉体的生命早期病因的检查，他ALTH问题已导致科学家在理解孩子们的反应，以唤起自适应响应，或者可能被认为是紧张的情况下挑战特别感兴趣。文献的优势是指这种现象为"压力反应"，虽然在遇到挑战性的刺激具有在宽范围的域的引出应答的潜力。因此，协议中所述下方被设计为跨多个领域，包括两种形式的休息引起ANS反应（一边听着舒缓的摇篮曲，并同时观看了冷静，中立视频）和18个月的孩子3发育相适应的挑战:预测/惊吓（玩偶的箱），感官（柠檬汁），和社会情感（听一个生病的婴儿哭声的）。该协议是改编自我们现有的12个月孩子8和3-5岁儿童11协议，以使其发育挑战性，参与和容忍的幼儿（18-21个月）。 在这里，我们目前的发展挑战协议（DCP）和ANS从应力的18个月大的考察访问数据，进食和孕妇和他们的后代的早期发展（SEED）研究。健康孕妇怀孕期间参加了2013-14孕期体重增加和产前应激的研究中招募和超重，低收入，以及种族和多族裔。刚出生后并再次之前的数据收集为这里所报告的研究中，当后代是18-21个月的年龄时2014-2015的开始获得父母对她的后代的研究的知情同意。

<table><tbody><tr><td>ANS equipment</td><td></td><td></td><td></td></tr><tr><td>Laptop and power cord&amp;nbsp;</td><td>Dell</td><td>60-0107-3.0</td><td>Mindware BioLab software requires a PC with 2 USB ports</td></tr><tr><td>ANS data collection software</td><td>Mindware</td><td>contact company</td><td>BioLab acquisition program</td></tr><tr><td>ANS data acquisition unit and cords</td><td>Mindware</td><td>50-3711-08</td><td>BioNex 8-slot Chassis</td></tr><tr><td>Snap leads</td><td>Mindware</td><td>93-0498-00</td><td>2 white, 2 red, 2 brown, 1 black</td></tr><tr><td>Spot electrodes</td><td>Kendall</td><td>31112496</td><td>130 model; 7 for each assessment + extras-to be sealed in a ziplock bag at all times)</td></tr><tr><td>ANS scoring/editing software</td><td>Mindware</td><td>contact company</td><td>HRV Analysis, version 3.1.0F and IMP Analysis, version 3.1.0I</td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Protocol equipment</td><td></td><td></td><td></td></tr><tr><td>tablet computer</td><td>Apple</td><td></td><td>iPad; for playing audio and video clips</td></tr><tr><td>portable speaker</td><td>Polariod</td><td>PBT510</td><td></td></tr><tr><td>Jack in the Box</td><td>Schylling</td><td>SMJB</td><td>Sock Monkey theme</td></tr><tr><td>Lemon juice</td><td>ReaLemon</td><td>98077</td><td>0.14 oz shelf-stable packets</td></tr><tr><td>Pasture pipettes</td><td>Grainger</td><td>6FAV9</td><td>1 ml size, disposable</td></tr><tr><td>Portion cups</td><td>Dart Solo Con</td><td>250PC</td><td>2.5 oz, disposable, plastic</td></tr><tr><td>audio files of baby cry</td><td>Alkon lab</td><td></td><td>30 sec clip recorded in a neonatal intensive care unit</td></tr><tr><td>audio file of baseline lullaby</td><td>Rockabye Baby Music</td><td></td><td>60 sec of &quot;Across the Universe&quot; on &quot;Rockabye Baby Lullaby Renditions of the Beatles&quot; CD</td></tr><tr><td>audio file of final lullaby</td><td>Rockabye Baby Music</td><td></td><td>60 sec of &quot;Here Comes the Sun&quot; on &quot;Rockabye Baby Lullaby Renditions of the Beatles&quot; CD</td></tr><tr><td>video file of neutral video</td><td></td><td></td><td>2 min of Baby Einstein video</td></tr><tr><td>Retractable measuring tape</td><td>Rollfix</td><td>TM04</td><td></td></tr><tr><td></td><td></td><td></td><td></td></tr><tr><td>Miscellaneous materials</td><td></td><td></td><td></td></tr><tr><td>Surge protector</td><td></td><td></td><td>to plug in all electronics</td></tr><tr><td>2-prong adapter for surge protector</td><td></td><td></td><td></td></tr><tr><td>Video camera</td><td>Sony</td><td></td><td>Handycam HDR-CX440</td></tr><tr><td>Video Encoder, power and input cords</td><td>Mindware</td><td>50-8614-01</td><td>optional; to sync video and ANS data files</td></tr><tr><td>Baby wipes and gauze</td><td></td><td></td><td>for cleaning and drying oily skin</td></tr><tr><td>Toys</td><td></td><td></td><td>to distract children during assessment</td></tr><tr><td>Visual aids</td><td></td><td></td><td>pictures of child with electrodes on, description of protocol to show participants</td></tr><tr><td>Script of protocol for research assistants</td><td></td><td></td><td></td></tr><tr><td>Charging cords for tablet and speaker</td><td></td><td></td><td></td></tr><tr><td>Doll and stickers</td><td></td><td></td><td>for demonstration if necessary</td></tr></tbody></table>

measuring cardiac autonomic nervous system (ans) activity in toddlers - resting and developmental challenges

自主神经系统（ANS）包括两个分支，副交感神经和交感神经系统，并控制内部器官（如心脏速率，呼吸，消化）的功能，并响应日常和不良事件1。已发现儿童ANS措施涉及到的行为问题，情绪调节和健康2-7。因此，幼儿期了解影响ANS发展的因素是很重要的。在ANS的两个分支影响青少年儿童对刺激心血管反应，并已测量无创性，通过外部监测设备，采用生理变化8-11有效和可靠的措施。不过，也有非常年幼的儿童与副交感神经和交感神经系统的同步措施，限制了两个系统的集成功能的了解一些研究。此外，Majori的现有幼儿的研究对婴幼儿休息ANS措施或者其反应报告TY常用的母子互动范例，而较少有人知道ANS反应到其他具有挑战性的条件。我们提出了一个研究设计和标准化的协议，用于心脏自主控制在18个月大的孩子一个非侵入性和快速评估。我们家庭或实验室访问期间描述了连续监测ANS的休息和挑战条件下，副交感神经和交感神经分支的方法，从我们的使用验证的设备和软件得分140种族多元化的幼儿提供样本描述调查结果。结果表明，该协议可能会产生一系列既休息和发育困难的条件下的生理反应，在心脏的变动和副交感神经和交感神经活动指数所示。个人休息的水平，respons证明变异ES挑战，挑战和反应性，它提供了额外的证据，该协议旨在为幼儿ANS个体差异考试有用。

种子研究纳入162母子二人组合（37％非裔美国人，30％的拉丁裔; 16％是白人，17％为其他种族或）。对于18月的访问中，我们完成了140名儿童（已登记的样本87％）的人的评价; 6名参加搬走了，剩下的参与者无法访问或这次访问不可用。为参观ANS数据收集组件的拒绝率是可供此访的母亲牌3/140（2％）。三次访问分别为21个月（超过3个月以后在目标年龄）后进行的，导致在135儿童在目标时间内收集的ANS的数据。差的信号质量得分从两个孩子的ANS数据使我们。因此，ANS的数据可用于133儿童。与这里提出ANS数据儿童的平均年龄为18.89个月（范围17.59-21.60; SD = 0.80），对儿童的53％是女性。意思是说，按性别和年龄调整后体重身长百分位数为68.40（SD = 27.47）。 与scoreable ANS数据133目标岁的儿童中，有100％的耐受性电极的应用也开始发展挑战的协议。该协议是为三个科目和五门科目宝宝哭任务后停产中途。两名受试者没有显示视频，由于设备的问题。从133儿童的全部信息进行评分。其中，三个孩子不得不因噪声/运动伪影无scoreable PEP的数据。四更多的孩子没有刻痕的第一个休息的摇篮曲数据PEP。七，其他孩子们基线摇篮曲在一个或多个片段unscorable PEP的数据。 为HR，RSA和由任务PEP措施描述统计列在表2 表2还给出在两个方面计算任务之间的平均变化:挑战任务反应计算（相对于静息水平DURING第一催眠曲）和任务到任务的变化计算（相对于前述任务）为每个ANS量度。那名显著不同于零的平均变化都指出， 图的挑战反应的人力资源，RSA和PEP三个领域5礼物盒状图。虽然平均反应值见表2和图5是小提出，绝大多数都是从零显著不同，以及箱线图显示所有任务一致的证据，所有ANS指数，儿童表现在他们对挑战作出反应的变化， 图6呈现平均的RSA和PEP响应于每个整个协议的任务，并表示当他们从任务到任务显著改变。 RSA大部分的相邻任务之间显著变化，这表明，平均来说，儿童的副交感神经系统的反应响应于每一个新的任务改变。该numbe用于PEP任务之间显著平均改变R的比用于RSA较少，虽然显著变化从摇篮曲到第一和第二个挑战的任务标识，并且从最后催眠曲到中立视频，这表明，平均来说，儿童的SNS反应是从静止到后续任务的条件不同。儿童年龄不显著任何挑战的反应成绩相关。这些模式表明，这一系列的任务引起的样品级变异整个协议和整个ANS的两个分支响应，并且，有一些任务的PNS与SNS分支之间不同的模式。 <img alt="图5" src="/files/ftp_upload/53652/53652fig5.jpg" /> 图5.箱图代表心率， 副交感神经， 交感神经 和 活性的发展挑战的任务。（A）表示听到吨率的反应性，（B）表示的RSA的反应性，和（C）代表的PEP的反应性。注意:框代表含有的值的中间50％四分位数间距，并在保持代表中位数的方块的行。晶须从最高值和最低值延长，不包括趴在箱外至少1.5箱长度异常值。 <a href="https://www.jove.com/files/ftp_upload/53652/53652fig5large.jpg" target="_blank">请点击此处查看该图的放大版本。</a> <img alt="图6" src="/files/ftp_upload/53652/53652fig6.jpg" /> 图6.交感神经和副交感神经活动跨越发展挑战议定书平均水平。在RSA（红色线）和PEP（蓝线）减少反映了分别通过PNS撤离和SNS激活，平均级别自主反应。 *表示平均得分样品为AN小号衡量这些相邻的任务（P &lt;0.05）之间的显著改变。 补充文件1:脚本A，B，C <a href="https://www.jove.com/files/ftp_upload/53652/Supplemental_File_1_JoVE_Bush2015_SupplementalFile_ScriptA,B,C_AA_edits.docx" target="_blank">，请点击这里下载该文件。</a> <img alt="表2" src="/files/ftp_upload/53652/53652tab2.jpg" /> 表2:描述性的ANS措施:任务级觉醒和两种差分成绩ANS响应表2显示了HR，RSA和PEP描述性统计。左列显示任务具体的统计数据，相对于静止的措施中间一栏显示结果挑战任务（指2摇篮曲的），而右列，用于从先前的任务每个任务的变化， <a href="https://www.jove.com/files/ftp_upload/53652/53652tab2large.jpg" target="_blank">请点击这里查看这个较大的FIGUR版本</a>即

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Measuring Cardiac Autonomic Nervous System ANS Activity in Toddlers - Resting and Developmental Challenges

我们描述了与18个月大的孩子休息和挑战的条件下连续监测的自主神经系统的方法。结果表明，该协议能产生有意义的生理反应的自主神经系统的两个分支和响应模式引起显著的个体差异。

测量心脏自主神经系统（ANS）活性的幼儿 - 安静和发展挑战

The autonomic nervous system (ANS) consists of two branches, the parasympathetic and sympathetic nervous systems, and controls the function of internal ...

measuring-cardiac-autonomic-nervous-system-ans-activity-toddlers

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Measuring Cardiac Autonomic Nervous System (ANS) Activity in Toddlers - Resting and Developmental Challenges

Biopsychology

The Autonomic Nervous System

SCIENTISTS IN ACTION

15.3K 次观看.  University of California, San Francisco. 我们描述了与18个月大的孩子休息和挑战的条件下连续监测的自主神经系统的方法。结果表明，该协议能产生有意义的生理反应的自主神经系统的两个分支和响应模式引起显著的个体差异。 

视频: Measuring Cardiac Autonomic Nervous System ANS Activity in Toddlers - Resting and Developmental Challenges

Quantitative Assessment of Cortical Auditory-tactile Processing in Children with Disabilities

Objective and easy measurement of sensory processing is extremely difficult in nonverbal or vulnerable pediatric patients. We developed a new methodology to quantitatively assess children&#39;s cortical processing of light touch, speech sounds and the multisensory processing of the 2 stimuli, without requiring active subject participation or causing children discomfort. To accomplish this we developed a dual channel, time and strength calibrated air puff stimulator that allows both tactile stimulation and sham control. We combined this with the use of event-related potential methodology to allow for high temporal resolution of signals from the primary and secondary somatosensory cortices as well as higher order processing. This methodology also allowed us to measure a multisensory response to auditory-tactile stimulation.

Objective and easy measurement of sensory processing is extremely difficult in nonverbal or vulnerable pediatric patients. We developed a new methodology to quantitatively assess infants and children&#39;s cortical processing of light touch, speech sounds, and the multisensory processing of the 2 stimuli, without requiring active subject participation or causing discomfort in vulnerable patients.

皮质听觉，触觉加工的残疾儿童定量评价

Objective and easy measurement of sensory processing is extremely difficult in nonverbal or vulnerable pediatric patients. We developed a new methodology ...

科研

行为学

Quantitative Autonomic Testing

Disorders associated with dysfunction of autonomic nervous system are quite common yet frequently unrecognized. Quantitative autonomic testing can be invaluable tool for evaluation of these disorders, both in clinic and research. There are number of autonomic tests, however, only few were validated clinically or are quantitative. Here, fully quantitative and clinically validated protocol for testing of autonomic functions is presented. As a bare minimum the clinical autonomic laboratory should have a tilt table, ECG monitor, continuous noninvasive blood pressure monitor, respiratory monitor and a mean for evaluation of sudomotor domain. The software for recording and evaluation of autonomic tests is critical for correct evaluation of data. The presented protocol evaluates 3 major autonomic domains: cardiovagal, adrenergic and sudomotor. The tests include deep breathing, Valsalva maneuver, head-up tilt, and quantitative sudomotor axon test (QSART). The severity and distribution of dysautonomia is quantitated using Composite Autonomic Severity Scores (CASS). Detailed protocol is provided highlighting essential aspects of testing with emphasis on proper data acquisition, obtaining the relevant parameters and unbiased evaluation of autonomic signals. The normative data and CASS algorithm for interpretation of results are provided as well.

Standardized, comprehensive and fully quantitative testing of autonomic functions is described. The autonomic tests consist of evaluation of all three major autonomic domains including cardiovagal, adrenergic and sudomotor. The severity and distribution of dysautonomia is quantitated using Composite Autonomic Severity Scores.

定量自主测试

Disorders associated with dysfunction of autonomic nervous system are quite common yet frequently unrecognized. Quantitative autonomic testing can be ...

医学

Infant Auditory Processing and Event-related Brain Oscillations

Rapid auditory processing and acoustic change detection abilities play a critical role in allowing human infants to efficiently process the fine spectral and temporal changes that are characteristic of human language. These abilities lay the foundation for effective language acquisition; allowing infants to hone in on the sounds of their native language. Invasive procedures in animals and scalp-recorded potentials from human adults suggest that simultaneous, rhythmic activity (oscillations) between and within brain regions are fundamental to sensory development; determining the resolution with which incoming stimuli are parsed. At this time, little is known about oscillatory dynamics in human infant development. However, animal neurophysiology and adult EEG data provide the basis for a strong hypothesis that rapid auditory processing in infants is mediated by oscillatory synchrony in discrete frequency bands. In order to investigate this, 128-channel, high-density EEG responses of 4-month old infants to frequency change in tone pairs, presented in two rate conditions (Rapid: 70 msec ISI and Control: 300 msec ISI) were examined. To determine the frequency band and magnitude of activity, auditory evoked response averages were first co-registered with age-appropriate brain templates. Next, the principal components of the response were identified and localized using a two-dipole model of brain activity. Single-trial analysis of oscillatory power showed a robust index of frequency change processing in bursts of Theta band (3 - 8 Hz) activity in both right and left auditory cortices, with left activation more prominent in the Rapid condition. These methods have produced data that are not only some of the first reported evoked oscillations analyses in infants, but are also, importantly, the product of a well-established method of recording and analyzing clean, meticulously collected, infant EEG and ERPs. In this article, we describe our method for infant EEG net application, recording, dynamic brain response analysis, and representative results.

High-density electroencephalography (dEEG) is being used increasingly to study brain development and plasticity in the early years of life. Here we present an application of sophisticated analysis techniques that builds on traditional EEG recording to understand the oscillatory dynamics of rapid auditory processing in the infant brain.

婴儿听觉处理和事件相关的脑振荡

Rapid auditory processing and acoustic change detection abilities play a critical role in allowing human infants to efficiently process the fine spectral ...

Eye Tracking Young Children with Autism

The rise of accessible commercial eye-tracking systems has fueled a rapid increase in their use in psychological and psychiatric research. By providing a direct, detailed and objective measure of gaze behavior, eye-tracking has become a valuable tool for examining abnormal perceptual strategies in clinical populations and has been used to identify disorder-specific characteristics1, promote early identification2, and inform treatment3. In particular, investigators of autism spectrum disorders (ASD) have benefited from integrating eye-tracking into their research paradigms4-7. Eye-tracking has largely been used in these studies to reveal mechanisms underlying impaired task performance8 and abnormal brain functioning9, particularly during the processing of social information1,10-11. While older children and adults with ASD comprise the preponderance of research in this area, eye-tracking may be especially useful for studying young children with the disorder as it offers a non-invasive tool for assessing and quantifying early-emerging developmental abnormalities2,12-13. Implementing eye-tracking with young children with ASD, however, is associated with a number of unique challenges, including issues with compliant behavior resulting from specific task demands and disorder-related psychosocial considerations. In this protocol, we detail methodological considerations for optimizing research design, data acquisition and psychometric analysis while eye-tracking young children with ASD. The provided recommendations are also designed to be more broadly applicable for eye-tracking children with other developmental disabilities. By offering guidelines for best practices in these areas based upon lessons derived from our own work, we hope to help other investigators make sound research design and analysis choices while avoiding common pitfalls that can compromise data acquisition while eye-tracking young children with ASD or other developmental difficulties.

Eye tracking has long been used to study gaze patterns in typically-developing individuals, but recent technological advancements have made its use with clinical populations, including autism, more feasible. While eye-tracking young children with autism can offer insight into early symptom manifestations, it involves methodological challenges. Suggestions for best practices are provided.

自闭症幼儿的眼动追踪

The rise of accessible commercial eye-tracking systems has fueled a rapid increase in their use in psychological and psychiatric research. By providing a ...

Psychophysiological Assessment of the Effectiveness of Emotion Regulation Strategies in Childhood

Effective regulation of emotion is one of the most important skills that develops in childhood. Research interest in this area is expanding, but empirical work has been limited by predominantly correlational investigations of children's skills. Relatedly, a key conceptual challenge for emotion scientists is to distinguish between emotion responding and emotion regulatory processes. This paper presents a novel method to address these conceptual and methodological issues in child samples. An experimental paradigm that assesses the effectiveness with which children regulate emotion is described. Children are randomly assigned to use specific emotion regulation strategies, negative emotions are elicited with film clips, and changes in subsequent psychophysiology index the extent to which emotion regulation is effective. Children are instructed to simply watch the emotion-eliciting film (control), distract themselves from negative emotions (cognitive distraction), or reframe the situation in a way that downplays the importance of the emotional event (cognitive reappraisal). Cardiac physiology, continuously acquired before and during the emotional task, serves as an objective measure of children's unfolding emotional responding while viewing evocative films. Key comparisons in patterns of obtained physiological reactivity are between the control and emotion regulation strategy conditions. Representative results from this approach are described, and discussion focuses on the contribution of this methodological approach to developmental science.

A detailed protocol for an experimental paradigm to assess the effectiveness with which children regulate emotion is described. Children are randomly assigned to use specific emotion regulation strategies, negative emotions are elicited with film clips, and changes in subsequent psychophysiology index the extent to which emotion regulation is effective.

情绪调节策略的童年有效性的心理生理评估

Effective regulation of emotion is one of the most important skills that develops in childhood. Research interest in this area is expanding, but empirical ...

Use of a Video Scoring Anchor for Rapid Serial Assessment of Social Communication in Toddlers

Reciprocal social behavior (RSB), an early-emerging capacity to engage in social contingency&#8212;which is foundational for both social learning and social competency&#8212;is hypothesized to be disrupted in autism spectrum disorder (ASD). The ability to quantify the full range of RSB during the toddler period, when core symptoms of ASD often arise, is pivotal for evaluating early risk for ASD, characterizing social development, and tracking response to early interventions. However, important parameters of variation in RSB&#8212;especially prior to the development of verbal language&#8212;can be nuanced and difficult to characterize using questionnaire-based methods. To address this challenge, we developed a system for measuring quantitative variation in RSB in toddlers (ages 18 - 30 months) that incorporated not only standard questionnaire data from caregivers but also a novel set of video-referenced items, through which a respondent compares the behavior of a subject to that observed in a short video of a young child manifesting a highly competent level of social communication. Testing of this measure in a general population sample of twins confirmed that both the video-referenced items and the RSB Total Score (video-referenced items plus non-video-referenced items) displayed unimodal, continuous distributions, strong internal consistency, marked preservation of individual differences, and extremely high heritability. In addition, video-referenced items were particularly sensitive to quantifying incremental changes in social communication, a major element of RSB, over the course of early childhood development. Scores on the vrRSB clearly differentiated children with and without ASD and these data comprise an initial validation of this promising method for quantifying early RSB&#8212;cross-sectionally, over time, and as a function of early intervention.

Here we present a protocol for administration of the video-referenced rating of reciprocal social behavior (vrRSB) using a paper or online version. This survey quantifies RSB, a prerequisite for social competence, in toddlers through &#34;video-referenced&#34; items whereby a subject is compared to a reference child shown in a three-minute video.

视频评分锚在幼儿社会交往快速评估中的应用

Reciprocal social behavior (RSB), an early-emerging capacity to engage in social contingency&#8212;which is foundational for both social learning and ...

Measuring Cardiac Autonomic Nervous System (ANS) Activity in Children

The autonomic nervous system (ANS) controls mainly automatic bodily functions that are engaged in homeostasis, like heart rate, digestion, respiratory rate, salivation, perspiration and renal function. The ANS has two main branches: the sympathetic nervous system, preparing the human body for action in times of danger and stress, and the parasympathetic nervous system, which regulates the resting state of the body.
ANS activity can be measured invasively, for instance by radiotracer techniques or microelectrode recording from superficial nerves, or it can be measured non-invasively by using changes in an organ's response as a proxy for changes in ANS activity, for instance of the sweat glands or the heart. Invasive measurements have the highest validity but are very poorly feasible in large scale samples where non-invasive measures are the preferred approach. Autonomic effects on the heart can be reliably quantified by the recording of the electrocardiogram (ECG) in combination with the impedance cardiogram (ICG), which reflects the changes in thorax impedance in response to respiration and the ejection of blood from the ventricle into the aorta. From the respiration and ECG signals, respiratory sinus arrhythmia can be extracted as a measure of cardiac parasympathetic control. From the ECG and the left ventricular ejection signals, the preejection period can be extracted as a measure of cardiac sympathetic control. ECG and ICG recording is mostly done in laboratory settings. However, having the subjects report to a laboratory greatly reduces ecological validity, is not always doable in large scale epidemiological studies, and can be intimidating for young children. An ambulatory device for ECG and ICG simultaneously resolves these three problems.
Here, we present a study design for a minimally invasive and rapid assessment of cardiac autonomic control in children, using a validated ambulatory device 1-5, the VU University Ambulatory Monitoring System (VU-AMS, Amsterdam, the Netherlands,<a href="http://www.vu-ams.nl" target="_blank"> www.vu-ams.nl</a>).

Measuring Cardiac Autonomic Nervous System ANS Activity in Children

Measurement of autonomic nervous system activity usually confines the researcher and participant to the laboratory, which may provide an intimidating environment to children. The VU University Ambulatory Monitoring System (VU-AMS) device can record cardiac autonomic control in any setting. The VU-AMS proved very amenable to testing in children.

测量心脏自主神经系统（ANS）儿童活动

The autonomic nervous system (ANS) controls mainly automatic bodily functions that are engaged in homeostasis, like heart rate, digestion, respiratory ...

An Electrophysiology Protocol to Measure Reward Anticipation and Processing in Children

We present a protocol designed to measure the neural correlates of reward in children. The protocol allows researchers to measure both reward anticipation and processing. Its purpose is to create a reward task that is appropriate for young children with and without autism while controlling reward properties between two conditions: social and nonsocial. The current protocol allows for comparisons of brain activity between social and nonsocial reward conditions while keeping the reward itself identical between conditions. Using this protocol, we found evidence that neurotypical children demonstrate enhanced anticipatory brain activity during the social condition. Furthermore, we found that neurotypical children anticipate social reward more robustly than children with autism diagnoses. As the task uses snacks as a reward, it is most appropriate for young children. However, the protocol may be adapted for use with adolescent or adult populations if snacks are replaced by monetary incentives. The protocol is designed to measure electrophysiological events (event-related potentials), but it may be customized for use with eye-tracking or fMRI.

This protocol is designed to measure reward anticipation and processing in young children with and without autism. Specifically, the protocol is designed to study the neural correlates of reward during social and nonsocial conditions while controlling for reward between conditions.

儿童奖励预期与处理的电生理学协议

We present a protocol designed to measure the neural correlates of reward in children. The protocol allows researchers to measure both reward anticipation ...

Calculating Heart Rate Variability from ECG Data from Youth with Cerebral Palsy During Active Video Game Sessions

The aim of this study was to generate a method for calculating heart rate variability (HRV) from electrocardiogram (ECG) waveforms. The waveforms were recorded by a HR monitor that participants (youth with cerebral palsy (CP)) wore during active video game (AVG) sessions. The AVG sessions were designed to promote physical activity and fitness (aerobic performance) in participants. The goal was to evaluate the feasibility of AVGs as a physical therapy (PT) intervention strategy. The maximum HR (mHR) was determined for each participant and the Target Heart Rate Zone (THRZ) was calculated for each of three exercise phases in the 20 min AVG session: (warm-up at 40-60% mHR, conditioning at 60-80% mHR, and cool down at 40-60% mHR). Each participant played three 20 min games during the AVG session. All games were played while sitting on a bench because many youth with CP cannot stand for extended periods of time. Each game condition differed with participants using hand icons only, hand and feet icons together or feet icons only to collect objects. The objective of the game (called KOLLECT) is to collect objects to gain points and avoid hazards to not lose points. Hazards were used in the warm-up and cool down phases only to promote slower, controlled movement to maintain HR in the target heart rate zone (THRZ). There were no hazards in the conditioning phase to promote higher levels and more intense physical activity. Analytic methods were used to generate HRV (selected time-domain and frequency-domain measures) from ECG data to examine aerobic workload. Recent applications of HRV indicate that short-term measurements (5 min bouts) are appropriate and that HRV biofeedback may help improve symptoms and the quality of life in a variety of health conditions. Although HR is a well-accepted clinical measure to examine aerobic performance and intensity in PT interventions, HRV may provide information of the autonomic system functions, recovery and adaptation during AVG sessions.

This protocol describes a method for calculating Heart Rate Variability (HRV) from electrocardiogram (ECG) waveforms. Waveforms from continuous heart rate (HR) recordings during active video game (AVG) sessions were used to measure the aerobic performance of youth with cerebral palsy (CP).

在活动视频游戏会话期间,从脑瘫青年的 ECG 数据计算心率变异性

The aim of this study was to generate a method for calculating heart rate variability (HRV) from electrocardiogram (ECG) waveforms. The waveforms were ...

A Familiarization Protocol Facilitates the Participation of Children with ASD in Electrophysiological Research

This paper includes a detailed description of a familiarization protocol, which is used as an integral component of a larger research protocol to collect electroencephalography (EEG) data and Event-Related Potentials (ERPs). At present, the systems available for the collection of high-quality EEG/ERP data make significant demands on children with developmental disabilities, such as those with an Autism Spectrum Disorder (ASD). Children with ASD may have difficulty adapting to novel situations, tolerating uncomfortable sensory stimuli, and sitting quietly. This familiarization protocol uses Evidence-Based Practices (EBPs) to increase research participants&#39; knowledge and understanding of the specific activities and steps of the research protocol. The tools in this familiarization protocol are a social narrative, a visual schedule, the Premack principle, role-playing, and modeling. The goal of this familiarization protocol is to increase understanding and agency and to potentially reduce anxiety for child participants, resulting in a greater likelihood of the successful completion of the research protocol for the collection of EEG/ERP data.

Electrophysiological research is an important tool for identifying biomarkers of developmental disorders, including Autism Spectrum Disorders (ASD), but data collection in these populations remains challenging. This work presents a familiarization protocol to accompany research that includes electroencephalography (EEG) to improve the likelihood of collecting EEG data from children with ASD.

熟悉方案有助于儿童参与电生理研究

This paper includes a detailed description of a familiarization protocol, which is used as an integral component of a larger research protocol to collect ...