Results: Ex vivo Imaging of Lung Metastasis and Their Microenvironment
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Conclusion
副本
The overall goal of this procedure is to visualize dynamic tumor cell stroma interactions within lung metastases.This method can help answer key questions in the cancer metastasis field, such as how are metastasis established.and what cell types w
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We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.