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Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

DOI :

10.3791/53741-v

February 3rd, 2016

February 3rd, 2016

12,104 Views

1Department of Anatomy, University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine

We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.

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Ex Vivo Live Imaging

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