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Isolation, Transfection, and Culture of Primary Human Monocytes

DOI :

10.3791/59967-v

December 16th, 2019

December 16th, 2019

12,571 Views

1Department of Medicine, Louisiana State University Health Sciences Center, 2Department of Microbiology, Immunology, and Parasitology, Louisiana State University Health Sciences Center, 3Department of Biomedical and Clinical Sciences L. Sacco, University of Milan

Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.

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Isolation

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