We thank Erin Bradley and Liz Cole for their assistance in the field. Field work, thin-section preparation and material for the sandbox model was supported by Franklin &amp; Marshall College's Committee on Grants.

<ol>
	<li>Marshak, S., Wilkerson, M. S., Hsui, H. T., KR, M. c. C. l. a. y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+curved+fold-thrust+belts:+Insights+from+simple+physical+and+analytical.">Generation of curved fold-thrust belts: Insights from simple physical and analytical.</a> modelsThrust Tectonics. , 83-92 (1992).</li><li>Mitra, G., S, S. e. n. g. u. p. t. a. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evolution+of+salients+in+a+fold-and-thrust+belt:+the+effects+of+sedimentary+basin+geometry,+strain+distribution+and+critical+taper.">Evolution of salients in a fold-and-thrust belt: the effects of sedimentary basin geometry, strain distribution and critical taper.</a> Evolution of Geological Structures in Micro- to Macro-scales. , 59-90 (1997).</li><li>Weil, A., Sussman, A., A, S. u. s. s. m. a. n., A, W. e. i. l. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Classifying+curved+orogens+based+on+timing+relationships+between+structural+development+and+vertical+axis+rotations.">Classifying curved orogens based on timing relationships between structural development and vertical axis rotations.</a> Orogenic curvature Geol. 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A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estimating+uncertainty+in+normalized+Fry+plots+using+a+bootstrap+approach.">Estimating uncertainty in normalized Fry plots using a bootstrap approach.</a> J. of Struct. Geol. 24 (2), 311-322 (2002).</li><li>De Paor, D. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Interactive+Program+for+Doing+Fry+Strain+Analysis+on+the+Macintosh+Microcomputer.">An Interactive Program for Doing Fry Strain Analysis on the Macintosh Microcomputer.</a> J. of Geol. Ed. 37 (3), 171-180 (1989).</li><li>Ismat, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Folding+kinematics+expressed+in+fracture+patterns:+An+example+from+the+Anti-Atlas+fold-belt,+Morocco.">Folding kinematics expressed in fracture patterns: An example from the Anti-Atlas fold-belt, Morocco.</a> J. of Struct. Geol. 30 (11), 1396-1404 (2008).</li><li>Reches, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Faulting+of+rocks+in+three-dimensional+strain+fields:+II.+Theoretical+analysis.">Faulting of rocks in three-dimensional strain fields: II. Theoretical analysis.</a> Tectonophys. 95 (1-2), 133-156 (1983).</li><li>Reches, Z., Dieterich, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Faulting+of+rocks+in+three+dimensional+strain+fields:+1.+Failure+of+rocks+in+polyaxial,+servo-control+experiments.">Faulting of rocks in three dimensional strain fields: 1. Failure of rocks in polyaxial, servo-control experiments.</a> Tectonophys. 95 (1-2), 111-132 (1983).</li><li>Ismat, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evolution+of+fracture+porosity+and+permeability+during+folding+by+cataclastic+flow:+Implications+for+syntectonic+fluid+flow.">Evolution of fracture porosity and permeability during folding by cataclastic flow: Implications for syntectonic fluid flow.</a> Rocky Mount. Geol. 47 (2), 133-155 (2012).</li><li>Kwon, S., Mitra, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+kinematic+history+at+an+oblique+ramp,+Leamington+zone,+Sevier+belt,+Utah.">Three-dimensional kinematic history at an oblique ramp, Leamington zone, Sevier belt, Utah.</a> J. of Struct. Geol. 28 (3), 474-493 (2006).</li><li>Casas, A. M., Simon, J. L., Seron, F. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stress+deflection+in+a+tectonic+compressional+field:+A+model+for+the+northeastern+Iberian+chain,+Spain.">Stress deflection in a tectonic compressional field: A model for the northeastern Iberian chain, Spain.</a> J. of Geophys. Res. 97, 7183-7192 (1992).</li><li>Apotria, T. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thrust+sheet+rotation+and+out-of-plane+strains+associated+with+oblique+ramps:+An+example.">Thrust sheet rotation and out-of-plane strains associated with oblique ramps: An example.</a> J. of Struct. Geol. 17 (5), 647-662 (1995).</li><li>Hubbert, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Theory+of+Scale+Models+as+Applied+to+the+Study+of+Geological+Structures.">Theory of Scale Models as Applied to the Study of Geological Structures.</a> Geol. Soc. of Am. Bull. 48 (10), 1459-1520 (1937).</li><li>Sch&#246;pfen, M. P. J., Steyrer, H. P., Koyi, H. 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Mem. 193, 21-27 (2001).</li><li>Kwon, S., Mitra, G., Sussman, A., Weil, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain+distribution,+strain+history+and+kinematic+evolution+associated+with+the+formation+of+arcuate+salients+in+fold-thrust+belts:+the+example+of+the+Provo+salient,+Sevier+orogeny,+Utah.">Strain distribution, strain history and kinematic evolution associated with the formation of arcuate salients in fold-thrust belts: the example of the Provo salient, Sevier orogeny, Utah.</a> Orogenic curvature Geol. Soc. of Am. Special Paper. 383, 205-223 (2004).</li><li>Elliott, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+motion+of+thrust+sheets.">The motion of thrust sheets.</a> J. of Geophys. Res. 81, 949-963 (1976).</li></ol>

The authors have nothing to disclose.

在塞维尔褶皱冲断带的中部犹他州段，其北界，在利明顿的横向区域作为理想的天然实验室，研究凸槽结（ 图1）。沿着这条交界处，输送方向保持恒定，并且推力片是跨过该结不间断，所以唯一变量是底层地下室几何5。 这里，我们提出通过组合与推块沙箱模型，它复制场区的大型几何现场收集多尺度故障数据以分析这种类型的凸凹结的方法。沙箱模型?...

褶皱冲断带是由salients（或段），其中在毗邻salients推力纸张由凹槽或横向区1,2,3脱钩的。从突出到凹陷的过渡可能会明显复杂，涉及到结构的多方面的套件，可以存放重要线索褶皱冲断带的发展。在本文中，我们仔细检查一个凸槽交界处，使用多尺度的现场数据和沙箱模型的组合，以更好地了解变形如何容纳褶皱冲断带之内。 中央犹他州段和利明横向区域的交界处是理想的天然实验室用于研究凸凹接合部有几个原因（ 图1）。首先，将段内露出的岩石继续，不中断，进入横向区4。所以，变形图案可以连续跟踪和穿过结比较。小号<html>的Econd，岩石基本上是单矿物，所以在故障模式的变化是不是单元内的非均质性的结果，而是反映了总体折叠和研究区域4内推压。第三，elastico-摩擦机制，如碎裂流，辅助变形整个场区，允许的尺度故障模式4直接比较。最后，总的输送方向保持沿着段和横向区域的长度连续的;因此，在缩短方向的变化不影响保留变形图案4。所有这些因素最小化的可能已影响到沿区段和横向区域中的变形的变量数。作为结果，我们推测，保留结构形成，主要是因为在底层基底几何学5的变化。 PLOAD / 54318 / 54318fig1.jpg"/&gt; 图1.索引图的例子。美国西部的塞维尔褶皱冲断带，呈现出大salients，段凹陷和横向的区域。由图2中方框区域（从伊斯马特·和Toeneboehn 7修改）表示。 <a href="https://www.jove.com/files/ftp_upload/54318/54318fig1large.jpg" target="_blank">请点击这里查看更大的版本这个数字。</a> 折叠和中央犹他州段和横利明顿区域内逆冲，发生在深度&lt;15公里处， 即 elastico-摩擦制度，其中主要的变形发生由露头尺度范围内（&lt;1米）断层和碎裂流4,6 。由于推力板的运输和折叠发生主要是由elastico-摩擦的机制，我们预测了详细的故障分析可提供进一步洞察利明顿的横向区域，次的运动历史<html> Ë底层地下室几何。为了检验这一假设，我们已收集并分析中央犹他州段的北部分内和整个利明横向区（ 图2）中的岩石保存故障模式。 <img alt="图3" src="/files/ftp_upload/54318/54318fig2.jpg" /> 图2.宏观尺度地形图的实施例。在图1中方框区域的上色浮雕地形图。4区域是由白色实线分开。元古宙球童峡谷石英岩（PCC）之间的床上用品的接触，相互元古代石英岩（PCM）和寒武纪Tintic石英岩（CT）所示。虚线显示山脉在此区域内的趋势。站点位置显示有编号的黑色方块。一阶线理都用实灰线（从伊斯马特·和Toeneboehn 7修改）。ftp_upload / 54318 / 54318fig2large.jpg"目标="_空白"&gt;点击此处查看该图的放大版本。 沙箱进行了实验来比较，和补充，故障数据。阿推块沙箱模型，额叶和倾斜坡道，被用来帮助我们保留在结构的分析，和周围的横向利明顿区（ 图3），7。这种方法的目标是四倍:1）确定尺度故障模式是一致的，2）确定所述沙箱模型支持并解释现场数据，3）确定如果沙箱模型提供在不属于结构的更多细节在现场观察到的，和4）评价该组合场实验方法是否是有用和容易复制。 <img alt="图3" src="/files/ftp_upload/54318/54318fig3.jpg" /> 图3的推块M实施例奥德尔。照片空沙箱模式。南部斜坡额叶（SFR），斜斜坡（OR），北斜前方（NFR），以及四个区域（1-4）被标记（由伊斯马特·和Toeneboehn 7修改）。 <a href="https://www.jove.com/files/ftp_upload/54318/54318fig3large.jpg" target="_blank">请点击此处查看大图这个数字。</a>

<table border="0" cellpadding="0" cellspacing="0" width="1362">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:227px;">fiberboard</td>
			<td style="width:140px;">Any</td>
			<td style="width:195px;">NA</td>
			<td style="width:800px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">finishing lacquer</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">epoxy</td>
			<td>Epoxy technology</td>
			<td>Parts A and B: 301-2 2LB</td>
			<td>best if warmed to 80&#186; - 125&#186;. If warming is not possible, it will cure fine, it will just take 1 week, rather than 1 day.</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">ramp wood-pine</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">painters tape</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">rabbit joints</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">countersunk fasteners</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">sand paper</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">play sand</td>
			<td>Any</td>
			<td>NA</td>
			<td>best if homogenous grain size, ~0.5mm</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">food coloring</td>
			<td>Any</td>
			<td>NA</td>
			<td>best to use one color and a dark color</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">plastic mesh/grid</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">square cross oins</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">crank screw</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">crank handle</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">sheet metal</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">dividers bars</td>
			<td>Any</td>
			<td>NA</td>
			<td></td>
		</tr>
	</tbody>
</table>

kinematic history of a salient-recess junction explored through a combined approach of field data and analog sandbox modeling

Within fold-thrust belts, the junctions between salients and recesses may hold critical clues to the overall kinematic history. The deformation history within these junctions is best preserved in areas where thrust sheets extend from a salient through an adjacent recess. We examine one such junction within the Sevier fold-thrust belt (western United States) along the Leamington transverse zone, northern Utah. Deformation within this junction took place by faulting and cataclastic flow. Here, we describe a protocol that examines these fault patterns to better understand the kinematic history of the field area. Fault data is supplemented by analog sandbox experiments. This study suggests that, in detail, deformation within the overlying thrust sheet may not directly reflect the underlying basement structure. We demonstrate that this combined field-experimental approach is easy, accessible, and may provide more details to the deformation preserved in the crust than other more expensive methods, such as computer modeling. In addition, the sandbox model may help to explain why and how these details formed. This method can be applied throughout fold-thrust belts, where upper-crustal rocks are well preserved. In addition, it can be modified to study any part of the upper crust that has been deformed via elastico-frictional mechanisms. Finally, this combined approach may provide more details as to how fold-thrust belts maintain critical-taper and serve as potential targets for natural resource exploration.

空中拍摄的照片被用于现场区域划分为四个区域（1-4），基于现代山嵴顶的趋势（ 图2）。多尺度故障数据这四个地区进行比较。假定这些趋势变化反映底层基底的几何形状，倾斜的斜坡位于区域2和3中，内，其中山趋势倾斜于塞维尔折叠推力带。在整个四个区域，我们发现，中尺度的故障保护变形织物是渗透和均匀的尺度（ 即，岩石的立方米），并代...

Watch this Scientific Journal Video about Kinematic History of a Salient-recess Junction Explored through a Combined Approach of Field Data and Analog Sandbox Modeling at JoVE.com

Kinematic History of a Salient-recess Junction Explored through a Combined Approach of Field Data and Analog Sandbox Modeling

Kinematic histories of fold-thrust belts are typically based on careful examinations of high-grade metamorphic rocks within a salient. We provide a novel method of understanding fold-thrust belts by examining salient-recess junctions. We analyze the oft-ignored upper crustal rocks using a combined approach of detailed fault analysis with experimental sandbox modeling.

通过现场数据和模拟沙盒建模相结合的办法探索出了一条凸槽结的运动史

Within fold-thrust belts, the junctions between salients and recesses may hold critical clues to the overall kinematic history. The deformation history ...

kinematic-history-salient-recess-junction-explored-through-combined

Research

JoVE Journal

Environment

8.1K 次观看.  Franklin and Marshall College. Kinematic histories of fold-thrust belts are typically based on careful examinations of high-grade metamorphic rocks within a salient. We provide a novel method of understanding fold-thrust belts by examining salient-recess junctions. We analyze the oft-ignored upper crustal rocks using a combined approach of detailed fault analysis with experimental sandbox modeling.

视频: Kinematic History of a Salient-recess Junction Explored through a Combined Approach of Field Data and Analog Sandbox Modeling

Surface Renewal: An Advanced Micrometeorological Method for Measuring and Processing Field-Scale Energy Flux Density Data

Advanced micrometeorological methods have become increasingly important in soil, crop, and environmental sciences. For many scientists without formal training in atmospheric science, these techniques are relatively inaccessible. Surface renewal and other flux measurement methods require an understanding of boundary layer meteorology and extensive training in instrumentation and multiple data management programs. To improve accessibility of these techniques, we describe the underlying theory of surface renewal measurements, demonstrate how to set up a field station for surface renewal with eddy covariance calibration, and utilize our open-source turnkey data logger program to perform flux data acquisition and processing. The new turnkey program returns to the user a simple data table with the corrected fluxes and quality control parameters, and eliminates the need for researchers to shuttle between multiple processing programs to obtain the final flux data. An example of data generated from these measurements demonstrates how crop water use is measured with this technique. The output information is useful to growers for making irrigation decisions in a variety of agricultural ecosystems. These stations are currently deployed in numerous field experiments by researchers in our group and the California Department of Water Resources in the following crops: rice, wine and raisin grape vineyards, alfalfa, almond, walnut, peach, lemon, avocado, and corn.

Surface renewal is a micrometeorological method that is being used increasingly to determine energy fluxes, but its technical complexity makes it inaccessible to a broad audience. We describe the steps needed to set up and calibrate a surface renewal field station, to acquire and process data, and to correctly interpret results.

表面重建:一种先进的微气象法测量和处理油田规模能流密度数据

Advanced micrometeorological methods have become  increasingly important in soil, crop, and environmental sciences.  For many scientists without formal ...

科研

环境科学

Extracting Metrics for Three-dimensional Root Systems: Volume and Surface Analysis from In-soil X-ray Computed Tomography Data

Plant roots play a critical role in plant-soil-microbe interactions that occur in the rhizosphere, as well as processes with important implications to climate change and crop management. Quantitative size information on roots in their native environment is invaluable for studying root growth and environmental processes involving plants. X-ray computed tomography (XCT) has been demonstrated to be an effective tool for in situ root scanning and analysis. We aimed to develop a costless and efficient tool that approximates the surface and volume of the root regardless of its shape from three-dimensional (3D) tomography data. The root structure of a Prairie dropseed (Sporobolus heterolepis) specimen was imaged using XCT. The root was reconstructed, and the primary root structure was extracted from the data using a combination of licensed and open-source software. An isosurface polygonal mesh was then created for ease of analysis. We have developed the standalone application imeshJ, generated in MATLAB1, to calculate root volume and surface area from the mesh. The outputs of imeshJ are surface area (in mm2) and the volume (in mm3). The process, utilizing a unique combination of tools from imaging to quantitative root analysis, is described. A combination of XCT and open-source software proved to be a powerful combination to noninvasively image plant root samples, segment root data, and extract quantitative information from the 3D data. This methodology of processing 3D data should be applicable to other material/sample systems where there is connectivity between components of similar X-ray attenuation and difficulties arise with segmentation.

A methodology for obtaining visual and quantitative root structure information from X-ray computed tomography data acquired in-soil is presented.

从在土X射线计算机断层扫描数据量和表面分析:提取指标三维根系

Plant roots play a critical role in plant-soil-microbe interactions that occur in the rhizosphere, as well as processes with important implications to ...

Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods

Rice fields host a large diversity of arthropods, but investigating their population dynamics and interactions is challenging. Here we describe the modification and application of a leaf blower-vac for suction sampling of arthropod populations in rice. When used in combination with an enclosure, application of this sampling device provides absolute estimates of the populations of arthropods as numbers per standardized sampling area. The sampling efficiency depends critically on the sampling duration. In a mature rice crop, a two-minute sampling in an enclosure of 0.13 m2 yields more than 90% of the arthropod population. The device also allows sampling of arthropods dwelling on the water surface or the soil in rice paddies, but it is not suitable for sampling fast flying insects, such as predatory Odonata or larger hymenopterous parasitoids. The modified blower-vac is simple to construct, and cheaper and easier to handle than traditional suction sampling devices, such as D-vac. The low cost makes the modified blower-vac also accessible to researchers in developing countries.

Assessment of arthropod abundance in crops is critical for investigating population dynamics and species interactions. Here we describe the modification and application of a leaf blower-vac for suction sampling of arthropods in rice.

修改和叶鼓风机-VAC的节肢动物领域采样中的应用

Rice fields host a large diversity of arthropods, but investigating their population dynamics and interactions is challenging. Here we describe the ...

Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions

We describe techniques for approximating seed bank dynamics over time using Helianthus annuus as an example study species. Strips of permeable polyester fabric and glue can be folded and glued to construct a strip of compartments that house seeds and identifying information, while allowing contact with soil leachate, water, microorganisms, and ambient temperature. Strips may be constructed with a wide range of compartment numbers and sizes and allow the researcher to house a variety of genotypes within a single species, different species, or seeds that have experienced different treatments. As opposed to individual seed packets, strips are more easily retrieved as a unit. While replicate packets can be included within a strip, different strips can act as blocks or can be retrieved at different times for observation of seed behavior over time. We used a high temperature glue gun to delineate compartments and sealed the strips once the seed and tags identifying block and removal times were inserted. The seed strips were then buried in the field at the desired depth, with the location marked for later removal. Burrowing animal predators were effectively excluded by use of a covering of metal mesh hardware cloth on the soil surface. After the selected time interval for burial, strips were dug up and seeds were assessed for germination, dormancy and mortality. While clearly dead seeds can often be distinguished from ungerminated living ones by eye, dormant seeds were conclusively identified using a standard Tetrazolium chloride colorimetric test for seed viability.

Here we present a protocol for assessing seed survivorship, germination and dormancy under field conditions using buried, labeled seed strips and tetrazolium chloride (TZ) viability testing.

对于在野外条件下评估种子萌发，休眠和死亡率可靠的方法

We describe techniques for approximating seed bank dynamics over time using Helianthus annuus as an example study species. Strips of permeable polyester ...

Field Experiments of Pollination Ecology: The Case of Lycoris sanguinea var. sanguinea

Plant-pollinator interactions have been studied for approximately one hundred years. During that time, many field methods have been developed to clarify the pollination effectiveness of each pollinator for visited flowers. Pollinator observations have been one of the most common methods to identify pollinators, and bagging and cage experiments have been conducted to show the effectiveness of specific pollinators. In a previous study of Lycoris sanguinea var. sanguinea, its effective pollinators, the visitation frequencies of each floral visitor, and its reproductive strategies were not identified. This study reports the observation that small bees visited flowers that were partially opened (breaking buds). To the best of our knowledge, this phenomenon has not been reported previously. Further, this study investigates the hypothesis that small bees can pollinate at that flowering stage. This study demonstrates the basic methods of field experiments in pollination ecology using L. sanguinea var. sanguinea. Pollinator observations and digital video showed the visitation frequencies of each floral visitor. Bagging and cage experiments revealed that these flowers could be pollinated fully and that breaking-bud pollination could be important for the pollination of this plant species. The advantages and disadvantages of each method are discussed, and recent developments, including laboratory experiments, are described.

Field Experiments of Pollination Ecology: The Case of Lycoris sanguinea var. sanguinea

To reveal the pollinator effectiveness of a given plant species, multiple methods of field experiments have been developed. This study demonstrates the basic methods of field experiments for pollination ecology using the case study of Lycoris sanguinea var. sanguinea and the novel pollination mechanism, breaking-bud pollination.

传粉生态学的田间试验:为例<em&gt;血红石蒜</em&gt;变种。<em&gt;荪</em

Plant-pollinator interactions have been studied for approximately one hundred years. During that time, many field methods have been developed to clarify ...

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

Recent studies demonstrated the use of environmental DNA (eDNA) from fishes to be appropriate as a non-invasive monitoring tool. Most of these studies employed disk fiber filters to collect eDNA from water samples, although a number of microbial studies in aquatic environments have employed filter cartridges, because the cartridge has the advantage of accommodating large water volumes and of overall ease of use. Here we provide a protocol for filtration of water samples using the filter cartridge and extraction of eDNA from the filter without having to cut open the housing. The main portions of this protocol consists of 1) filtration of water samples (water volumes &#8804;4 L or &gt;4 L); (2) extraction of DNA on the filter using a roller shaker placed in a preheated incubator; and (3) purification of DNA using a commercial kit. With the use of this and previously-used protocols, we perform metabarcoding analysis of eDNA taken from a huge aquarium tank (7,500 m3) with known species composition, and show the number of detected species per library from the two protocols as the representative results. This protocol has been developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.

滤芯水过滤样品和环境DNA的提取使用

Recent studies demonstrated the use of environmental DNA (eDNA) from fishes to be appropriate as a non-invasive monitoring tool. Most of these studies ...

Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance

A major concern when developing commercial mycoinsecticides is the kill speed compared to that of chemical insecticides. Therefore, isolation and screening for the selection of a fast-acting, highly virulent entomopathogenic fungus are important steps. Entomopathogenic fungi, such as Metarhizium, Beauveria, and Nomurea, which act by contact, are better suited than Bacillus thuringiensis or nucleopolyhedrosis virus (NPV), which must be ingested by the insect pest. In the present work, we isolated 68 Metarhizium strains from infected insects using a soil dilution and bait method. The isolates were identified by the amplification and sequencing of the ITS1-5.8S-ITS2 and 26S rDNA region. The most virulent strain of Metarhizium anisopliae was selected based on the median lethal concentration (LC50) and time (LT50) obtained in insect bioassays against III-instar larvae of Helicoverpa armigera. The mass production of spores by the selected strain was carried out with solid-state fermentation (SSF) using rice as a substrate for 14 days. Spores were extracted from the sporulated biomass using 0.1% tween-80, and different formulations of the spores were prepared. Field trials of the formulations for the control of an H. armigera infestation in pigeon peas were carried out by randomized block design. The infestation control levels obtained with oil and aqueous formulations (78.0% and 70.9%, respectively) were better than the 63.4% obtained with chemical pesticide.

Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance

Here, we report the different stages involved in the knowledge-based development of an effective mycoinsecticide, including the isolation, identification, screening, and selection of the "best-fit" entomopathogenic fungus, Metarhizium anisopliae, for the control of insect pests in agriculture.

的发展<em&gt; Metarhizium anisopliae</em&gt;作为防腐剂:从隔离到现场表现

A major concern when developing commercial mycoinsecticides is the kill speed compared to that of chemical insecticides. Therefore, isolation and ...

Experimental Methods of Dust Charging and Mobilization on Surfaces with Exposure to Ultraviolet Radiation or Plasmas

Electrostatic dust transport has been hypothesized to explain a number of observations of unusual planetary phenomena. Here, it is demonstrated using three recently developed experiments in which dust particles are exposed to thermal plasma with beam electrons, beam electrons only, or ultraviolet (UV) radiation only. The UV light source has a narrow bandwidth in wavelength centered at 172 nm. The beam electrons with the energy of 120 eV are created with a negatively biased hot filament. When the vacuum chamber is filled with the argon gas, a thermal plasma is created in addition to the electron beam. Insulating dust particles of a few tens of microns in diameter are used in the experiments. Dust particles are recorded to be lofted to a height up to a few centimeters with a launch speed up to 1 m/s. These experiments demonstrate that photo and/or secondary electron emission from a dusty surface changes the charging mechanism of dust particles. According to the recently developed &#34;patched charge model&#34;, the emitted electrons can be re-absorbed inside microcavities between neighboring dust particles below the surface, causing the accumulation of enhanced negative charges on the surrounding dust particles. The repulsive forces between these negatively charged particles may be large enough to mobilize and lift them off the surface. These experiments present the advanced understanding of dust charging and transport on dusty surfaces, and laid a foundation for future investigations of its role in the surface evolution of airless planetary bodies.

Dust charging and mobilization is demonstrated in three experiments with exposure to thermal plasma with beam electrons, beam electrons only, or ultraviolet (UV) radiation only. These experiments present the advanced understanding of electrostatic dust transport and its role in shaping the surfaces of airless planetary bodies.

紫外辐射或等离子体照射表面的粉尘充电和动员实验方法

Electrostatic dust transport has been hypothesized to explain a number of observations of unusual planetary phenomena. Here, it is demonstrated using ...

A Video Surveillance System to Monitor Breeding Colonies of Common Terns (Sterna Hirundo)

Many waterbird populations have faced declines over the last century, including the common tern (Sterna hirundo), a waterbird species with a widespread breeding distribution, that has been recently listed as endangered in some habitats of its range. Waterbird monitoring programs exist to track populations through time; however, some of the more intensive approaches require entering colonies and can be disruptive to nesting populations. This paper describes a protocol that utilizes a minimally invasive surveillance system to continuously monitor common tern nesting behavior in typical ground-nesting colonies. The video monitoring system utilizes wireless cameras focused on individual nests as well as over the colony as a whole, and allows for observation without entering the colony. The video system is powered with several 12 V car batteries that are continuously recharged using solar panels. Footage is recorded using a digital video recorder (DVR) connected to a hard drive, which can be replaced when full. The DVR may be placed outside of the colony to reduce disturbance. In this study, 3,624 h of footage recorded over 63 days in weather conditions ranging from 12.8 &#176;C to 35.0 &#176;C produced 3,006 h (83%) of usable behavioral data. The types of data retrieved from the recorded video can vary; we used it to detect external disturbances and measure nesting behavior during incubation. Although the protocol detailed here was designed for ground-nesting waterbirds, the principal system could easily be modified to accommodate alternative scenarios, such as colonial arboreal nesting species, making it widely applicable to a variety of research needs.

A Video Surveillance System to Monitor Breeding Colonies of Common Terns Sterna Hirundo

This paper describes a protocol that uses a remote video monitoring surveillance system to continuously monitor breeding colonies of ground-nesting waterbirds. The system includes five cameras monitoring individual nests and one camera monitoring the colony as a whole, and is powered by car batteries that are recharged via solar panels.

监测常见燕鸥繁殖菌落的视频监控系统 (燕鸥家燕)

Many waterbird populations have faced declines over the last century, including the common tern (Sterna hirundo), a waterbird species with a widespread ...

Construction of a Low-cost Mobile Incubator for Field and Laboratory Use

Incubators are essential for a range of culture-based microbial methods, such as membrane filtration followed by cultivation for assessing drinking water quality. However, commercially available incubators are often costly, difficult to transport, not flexible in terms of volume, and/or poorly adapted to local field conditions where access to electricity is unreliable. The purpose of this study was to develop an adaptable, low-cost and transportable incubator that can be constructed using readily available components. The electronic core of the incubator was first developed. These components were then tested under a range of ambient temperature conditions (3.5 &#176;C - 39 &#176;C) using three types of incubator shells (polystyrene foam box, hard cooler box, and cardboard box covered with a survival blanket). The electronic core showed comparable performance to a standard laboratory incubator in terms of the time required to reach the set temperature, inner temperature stability and spatial dispersion, power consumption, and microbial growth. The incubator set-ups were also effective at moderate and low ambient temperatures (between 3.5 &#176;C and 27 &#176;C), and at high temperatures (39 &#176;C) when the incubator set temperature was higher. This incubator prototype is low-cost (&lt; 300 USD) and adaptable to a variety of materials and volumes. Its demountable structure makes it easy to transport. It can be used in both established laboratories with grid power or in remote settings powered by solar energy or a car battery. It is particularly useful as an equipment option for field laboratories in areas with limited access to resources for water quality monitoring.

This paper describes a method for building an adaptable, low-cost and transportable incubator for microbial testing of drinking water. Our design is based on widely available materials and can operate under a range of field conditions, while still offering the advantages of higher-end laboratory-based models.

建造供野外和实验室使用的低成本移动孵化器

Incubators are essential for a range of culture-based microbial methods, such as membrane filtration followed by cultivation for assessing drinking water ...