Homogeneous Time Resolved Fluorescence cAMP Detection Assay
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Results: Potency Determination Assay Using Acoustic Transfer to Serially Dilute Peptide Agonists
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Conclusion
副本
The overall goal of this automated protocol for the serial dilution of peptide samples is to minimize peptide absorption to plasticware enabling accurate potency determination for drug discovery. This method can increase the quality and reproducib
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Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.