Experimental L. monocytogenes Inoculum Preparation and Infection
3:44
Peak Infection Spleen and Liver Bacterial Load Measurement
6:16
Effects of L. monocytogenes on CD4+ and CD8+ Cell IFN-gamma Responses
7:43
Results: Representative NK, NKT, and CD4 and CD8 T cell IFN-gamma Responses to L. monocytogenes
8:54
Conclusion
副本
The overall goals of this protocol are to cultivate and infect C57 blacksix mice with the EGG strain of listeria monocytogenes and to measure the bacterial load, interferon gamma responses, and survival and response to infection by this pathogen.
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This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.