Fluorescence Loss in Photobleaching Assay to Assess Mitochondrial Matrix Continuity
3:34
Live Staining with Fluorescent Mitochondrial Dyes
5:13
Co-immunostaining for Cyclin E and Mitochondria
7:48
Results: Live Microscopy of the Drosophila Ovary
9:19
Conclusion
副本
The overall goal of this procedure, is to demonstrate methods for studying mitochondrial structure and function in live as well as in fixed tissue in the model organism drosophila melanogaster. This method can help answer key questions in the fiel
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Analysis of the mitochondrial structure-function relationship is required for a thorough understanding of the regulatory mechanisms of mitochondrial functionality. Specific methods for studying mitochondrial structure and function in live and fixed Drosophila ovaries are described and demonstrated in this paper.