Embedding Embryos for Inverted or Upright Microscopy
2:36
Removal of Excess Agarose Around the Posterior Body
4:03
Mounting of Embryos for Vertical Light-sheet Microscope
5:05
Results: Long-term Time Lapse Imaging of Mounted Zebrafish Embryos
6:15
Conclusion
Transcript
Overall goal of this method is to mount Zebrafish embryos for live imaging, that enables the normal outgrowth of the posterior body region. This method can be adapted for the imaging of other regions of the developing Zebrafish. This technique hel
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Here, we present a versatile mounting method that allows for the long-term time-lapse imaging of the posterior body development of live zebrafish embryos without perturbing normal development.
