这项工作得到了 JCO a * 星 Investigatorship 赠款 (项目号 1437C00135), 一个 * 星科学 & 工程研究理事会公共部门资助 (项目 1421200076), 和一个 jsp-新加坡国立大学联合研究项目赠款 (WBS R143-000-611-133) 支持。

1. 预备前体库存解决方案
<ol><li>
顶级股票解决方案。
	<ol>
<li>在氮气气氛手套中, 将11.84 克硒小球称量成150毫升的圆锥烧瓶, 并在烧瓶中放置一个磁力搅拌棒。</li>
		<li>将100毫升的三氮辛基 (顶部) 加到圆锥烧瓶中, 用橡胶隔膜密封烧瓶。</li>
		<li>搅拌的混合物隔夜在 800 RPM。 注: 一旦硒颗粒完全溶解在顶部, 顶部的溶液 (1.5 米) 就可以用于进一步的反应。</li>
	</ol>
</li>
	<li>
顶部的股票解决方案。
	<ol>
<li>将0.611 克的硫粉称量成20毫升的 single-necked 圆底烧瓶 (RBF), 并在烧瓶中放置一个磁力搅拌棒。</li>
		<li>加入10毫升的硫粉和密封的径向基带橡胶隔膜。放置在氮气的气氛下, 搅拌 800 RPM。</li>
		<li>一旦硫粉溶解, 解决方案是明确的, 使用顶部的股票解决方案在进一步的反应。</li>
	</ol>
</li>
</ol>2. CdSe 量子点的合成
<ol><li>
矿 CdSe 量子。 注意: 请参见5。<ol>
<li>将9克三氮辛基氧化物 (拓扑)、6克十六烷基胺 (HDA) 和0.25 毫升 diisooctylphosphonic 酸 (蒂帕) 混合在三颈径向基函数中。在混合物中加入磁性搅拌棒, 并通过刺穿的橡胶隔膜将温度探头插入 RBF。<ol>
<li>在 RBF 上安装回流冷凝器, 并通过适配器将其连接到 Schlenk 线。在所有 glass-to-glass 接头处应用高真空润滑脂。</li>
		</ol>
</li>
		<li>加气溶液在100° c 为 2 h 在恒定的搅动下在800转每分钟。</li>
		<li>分别将乙酰丙酮镉 (Cd (民航)2)、0.570 克 hexadecanediol (HDDO) 和6毫升 1-十八 (颂歌) 混合在 single-necked 径向基函数中。在120° c 和 800 RPM 的2小时内, 在油浴中加入磁搅拌棒和加气搅拌。</li>
		<li>在排气2小时后, 将三颈径向基函数的温度提高到345° c。同时, 让镉前体混合物冷却到室温, 并在 single-necked 径向基函数中加入4毫升的顶硒库存溶液。</li>
		<li>一旦温度达到345° c, 就迅速将整个镉和硒前驱体溶液注入三颈径向基函数。在注入前驱体溶液后立即取出加热地幔, 并允许反应混合物在室温条件下冷却至室温。</li>
		<li>将15毫升的甲苯添加到原油反应混合物中, 并将合成的量子离心为3分钟, 在 2240 x g 处除去沉淀, 并在回收的上清液中加入过量的甲醇。离心混合物在 2325 x g 为8分钟和放弃上清液。</li>
		<li>将5毫升的甲苯添加到沉淀中, 以分散量子. 利用30毫升甲醇进行量子沉淀的进一步处理周期, 将悬浮液在 2240 x g 上离心3分钟, 丢弃上清, 在5毫升的甲苯中分散量子。<ol>
<li>经过两个处理周期后, 将量子的最小用量 (约2-3 毫升) 分散到甲苯中 (此后称为 "量子数库存溶液") 以供进一步使用。</li>
		</ol>
</li>
		<li>采用20µL 的溶液, 并加入3毫升的甲苯, 确定溶液中量子的浓度。测量 350 nm 的吸光度, 并使用已知的摩尔吸收率在 350 nm6上计算浓度。相应地缩放浓度, 以考虑到库存溶液的稀释。 注: 重要的是要保持积极的解决方案, 并立即删除加热地幔后, 迅速注入的前体溶液在步骤 2.1.5, 以获得高尺寸单分散的量子。</li>
	</ol>
</li>
	<li>
锌-闪锌矿 CdSe 量子。 注意: 请参见7。<ol>
<li>将0.0384 克的氧化镉 (CdO)、0.137 克肉豆蔻酸 (MA) 和5毫升的颂歌混合在三颈径向基函数中。在混合物中加入磁性搅拌棒, 并通过刺穿的橡胶隔膜将温度探头插入 RBF。在 RBF 上安装回流冷凝器, 并通过适配器将其连接到 Schlenk 线。用橡皮隔膜密封剩余的端口。在所有 glass-to-glass 接头处应用高真空润滑脂。</li>
		<li>加气溶液在90° c 下恒定搅拌在 800 RPM。</li>
		<li>分别, 加气0.05 毫升油酸 (OA), 0.5 毫升的油 (OM) 和2毫升的颂歌在 single-necked 径向基函数在恒定搅拌下在75° c 和 800 RPM。</li>
		<li>将镉前驱体溶液的温度提高到250° c, 并保持 15 min. 观察溶液中的颜色变化, 从深褐色到无色, 表明镉酸的形成。</li>
		<li>冷却反应混合物到90° c 并且增加12毫升的颂歌。再加气1小时。</li>
		<li>让反应混合物冷却到室温。加入0.012 克硒粉和加气混合物20分钟。</li>
		<li>在 N2下, 将反应混合物的温度提高到240° c。观察颜色变化, 从无色到黄色在150° c 然后到橙色红色在到达240° c, 意味形成 zb-CdSe 量子。</li>
		<li>在240° c 下长出5分钟的量子, 然后将混合物冷却到室温。将 OM、OA 和滴的脱混合物添加到反应溶液中。在室温条件下使溶液冷却至室温。</li>
		<li>通过在生长液中加入丙酮来处理合成的量子, 直到总体积约50毫升。离心结果悬浮在 2240 x g 为 3 min。随后, 丢弃上清液。</li>
		<li>在沉淀中加入5毫升的甲苯, 以分散量子. 进行进一步的处理周期: 用30毫升的甲醇沉淀量子, 将悬浮液在 2240 x 克上离心3分钟, 丢弃上清, 在5毫升的甲苯中分散量子。<ol>
<li>经过两个处理周期后, 将量子的最小用量 (约2-3 毫升) 分散到甲苯中 (此后称为 "量子数库存溶液") 以供进一步使用。</li>
		</ol>
</li>
		<li>采用20µL 处理后的纳米颗粒, 加入3毫升的甲苯, 确定了 CdSe 量子在股票溶液中的浓度。测量 350 nm 的吸光度, 并使用已知的摩尔吸收率在 350 nm6上计算浓度。相应地缩放浓度, 以考虑到库存溶液的稀释。 注: 对于单分散量子的成功合成, 关键是要确保所有的硒粉加入到反应混合物中, 并且它的所有都不留在径向基函数的颈部和侧。</li>
	</ol>
</li>
</ol>3. CdSe 种子 CdS NRs 的合成
注意: 请参见8。
<ol><li>混合3克拓扑, 0.90 克 CdO, 0.80 克 hexylphosphonic 酸 (HPA), 和0.29 克 octadecylphosphonic 酸 (ODPA) 在50毫升三-颈径向基函数。在混合物中加入磁性搅拌棒, 并通过刺穿的橡胶隔膜插入温度探头。</li>
	<li>在 RBF 上安装回流冷凝器, 并通过适配器将其连接到 Schlenk 线。用橡皮隔膜密封剩余的端口。在所有 glass-to-glass 接头处应用高真空润滑脂。将径向基函数加热至150° c, 并将其置于真空下 1.5 h 至加气, 搅拌在 800 RPM。</li>
	<li>放置1.8 毫升的顶部的股票解决方案成一个10毫升 single-necked 径向基函数和密封它与橡胶隔膜。在甲苯中加入 80 nmol 矿 CdSe 量子, 然后在70° c 真空下除去甲苯。让德加的解决方案在 800 RPM 下搅拌30分钟。</li>
	<li>将含有该 Cd 前驱体的径向基函数置于氮气下, 并将温度升高到350° c。在320° c, 通过橡胶隔膜将1.8 毫升的顶部添加到 RBF。</li>
	<li>在350° c 时, 将含有 w CdSe 量子的顶部溶液绘制成注射器, 并迅速注入含有该 Cd 前驱体的径向基函数。允许的解决方案搅拌在 800 RPM 额外的6分钟, 以允许增长的 NRs。随后, 在环境条件下, 除去加热地幔, 冷却溶液至室温。</li>
	<li>为了处理 NRs 的溶液, 在生长液中加入2毫升的甲苯, 并将整个混合物放入50毫升离心管中。加入30毫升的甲醇。随后, 离心结果悬浮在 2240 x g 为3分钟, 然后丢弃上清液。</li>
	<li>在沉淀中加入5毫升的甲苯, 以分散 NRs. 进行进一步的处理周期: 用30毫升的甲醇沉淀 NRs, 将悬浮液在 2240 x 克上离心3分钟, 丢弃上清, 在5毫升的甲苯中分散 NRs。<ol>
<li>经过2-3 次加工周期, 将 NRs 分散在5毫升甲苯中 (此后称为 NR 溶液), 以供进一步使用。</li>
	</ol>
</li>
	<li>准备样品进行 TEM 分析。5<ol>
<li>将一滴 NR 溶液放在一个覆盖有连续碳膜的300网状铜网格上进行电子显微镜分析。</li>
		<li>用吸附剂纸除去多余的溶液, 在室温下烘干样品。</li>
	</ol>
</li>
	<li>进行 TEM 成像 (和分析), 以获得 nr 的尺寸, 使 nr 的平均体积和每 nr 的摩尔数可以确定。<ol>
<li>采用20µL 处理后的 NRs, 并将其添加到3毫升的甲苯中, 确定了 CdSe 种子 CdS NRs 在股票溶液中的浓度。测量 350 nm 的吸光度, 用已知的摩尔吸收率计算 NRs 的浓度 (假定为由 cd 控制)9。相应地对浓度进行缩放, 以解释库存溶液的稀释。 注: 对于一个典型的合成, NRs 的产量接近 100%, 很少有副产品, 如分支结构。</li>
	</ol>
</li>
</ol>4. CdSe 种子 CdSe NRs 的合成
注意: 请参见8。
<ol><li>混合1.035 克的 CdO, 0.1657 克 HPA, 0.1543 克 n-四酸 (TDPA), 和3克拓扑在三颈径向基函数。在混合物中加入磁性搅拌棒, 并通过刺穿的橡胶隔膜将温度探头插入 RBF。在 RBF 上安装回流冷凝器, 并通过适配器将其连接到 Schlenk 线。用橡皮隔膜密封剩余的端口。在所有 glass-to-glass 接头处应用高真空润滑脂。</li>
	<li>加气溶液在150° c 为 1.5 h 在恒定的搅动下在800转每分钟。</li>
	<li>另外, 混合2毫升的 TOPSe 库存溶液和 10 nmol 的 w CdSe 量子 (分散在甲苯) 在 single-necked 径向基函数和德加在一个油浴在 800 RPM 和90° c 的常数搅拌下, 直到所有的甲苯被删除。</li>
	<li>脱气后, 将镉前体混合物的温度提高到340° c。随着温度的升高, 观察溶液中的颜色变化, 从深褐色到无色, 表明镉-烷基膦酸络合物的形成。</li>
	<li>当镉的前驱温度达到340° c 时, 迅速地将1.8 毫升的顶端注入混合物中, 使温度恢复到340° c。</li>
	<li>在到达340° c 时, 迅速地向三颈径向基函数注入1.8 毫升的顶端硒/CdSe 量子混合物, 温度将降到320° c。在恢复到340° c 时, 保持反应溶液在这个温度为 ~ 3 分钟在去除加热的披风之前和允许解答冷却到室温在环境条件下。</li>
	<li>在反应溶液冷却到室温后, 加入2毫升的甲苯并将整个溶液转移到50毫升离心管上。上离心管与甲醇 (99%) 到50毫升和离心结果悬浮在 2240 x g 为 3 min。随后, 丢弃上清液。</li>
	<li>在沉淀中加入5毫升的甲苯以分散 NRs. 进行进一步的处理周期: 沉淀 NRs 使用30毫升甲醇 (99%), 离心悬浮在 2240 x g 为3分钟, 丢弃上清, 并分散 NRs 5 毫升的甲苯。<ol>
<li>经过2-3 次加工周期, 将 NRs 分散在5毫升甲苯中 (此后称为 NR 溶液), 以供进一步使用。</li>
	</ol>
</li>
	<li>将一滴 NR 溶液放在覆盖有连续碳膜的300网状铜网格上进行电子显微镜分析。用吸附剂纸除去多余的溶液, 在室温下烘干样品。</li>
	<li>进行 TEM 成像和分析, 得到 NRs 的尺寸, 从而确定 nr 的平均体积和每 nr CdSe 的摩尔数。<ol>
<li>采用20µL 的溶液, 并加入3毫升的甲苯, 确定溶液中 NRs 的浓度。测量 350 nm 的吸光度, 并使用已知的摩尔吸收率在该波长6上计算浓度。相应地缩放浓度, 以考虑到库存溶液的稀释。 注: 对于一个典型的合成, NRs 的产量接近 100%, 很少有副产品, 如分支结构。</li>
	</ol>
</li>
</ol>5. CdSe 种子 CdSe TPs 的合成
注意: 请参见10。
<ol><li>混合0.43 克 CdO, 3.8 毫升 OA, 2.2 毫升颂歌, 和0.3 毫升的顶部在三颈径向基函数。在混合物中加入磁性搅拌棒, 并通过刺穿的橡胶隔膜将温度探头插入 RBF。在 RBF 上安装回流冷凝器, 并通过适配器将其连接到 Schlenk 线。用橡皮隔膜密封剩余的端口。在所有 glass-to-glass 接头处应用高真空润滑脂。</li>
	<li>加气溶液在90° c 为 1 h 在恒定的搅动下在800转每分钟然后增加温度到265° c。当温度达到265° c 时, 观察溶液颜色的变化, 从深褐色到无色, 表明油酸镉的形成复杂。</li>
	<li>将温度降低到50° c, 加入1.7 毫升的2米 TOPSe (分别由 sonicating、37赫和 320 W 组成, 在0.316 毫升的玻璃瓶中2毫升的混合物中8克硒粉) 和0.017 克甲基溴 (CTAB)。</li>
	<li>另外, 混合7毫升的颂歌, 0.025 克 CTAB, 0.5 毫升的顶部, 0.75 毫升 OA, 和 100 nmol 锌闪锌矿 CdSe 量子 (分散在甲苯) 在三颈径向基函数。在混合物中加入磁性搅拌棒, 并通过刺穿的橡胶隔膜将温度探头插入 RBF。<ol>
<li>在 RBF 上安装一个冷凝器, 并通过适配器将它连接到 Schlenk 线。用橡皮隔膜密封剩余的端口。在所有 glass-to-glass 接头处应用高真空润滑脂。</li>
	</ol>
</li>
	<li>德加在步骤5.4 中的混合物在90° c 和 800 RPM 下不断搅拌为〜45分钟, 直到所有的甲苯被删除。</li>
	<li>将含有锌-闪锌矿 CdSe 量子的反应混合物的温度提高到260° c。当温度达到260° c 时, 用注射器泵将8毫升的镉前驱体溶液添加到含有锌-闪锌矿 CdSe 量子的混合物中, 其注射速率为0.25 毫升/分钟。添加完成后, 在室温条件下使溶液冷却至室温。</li>
	<li>解决方案冷却到室温后, 将其转移到50毫升离心管。加入40毫升丙酮 (99%) 沉淀 TPs 和离心悬浮在 1340 x g 为10分钟。随后, 丢弃上清液。</li>
	<li>将5毫升的甲苯添加到 TPs 的沉淀中, 以分散它们。进行进一步的处理周期: 沉淀 tps 使用30毫升甲醇 (99%), 离心悬浮在 2240 x g 为3分钟, 丢弃上清, 并分散 tps 在5毫升的甲苯。<ol>
<li>在处理2-3 循环后, 将 TPs 分散在5毫升甲苯 (以后称为 TP 库存溶液) 中以供进一步使用。</li>
	</ol>
</li>
	<li>将 TP 溶液放在一个覆盖有连续碳膜的300网状铜网格上, 用于电子显微镜分析。用吸附纸除去多余的溶液, 在室温下烘干样品。</li>
	<li>进行 TEM 成像和分析, 以获得 TPs 的尺寸, 使 tp 的平均体积和每 tp 的 CdSe 摩尔数可以确定。<ol>
<li>采用20µL 的溶液, 并加入3毫升的甲苯, 确定溶液中量子的浓度。</li>
		<li>测量 350 nm 的吸光度, 并使用已知的摩尔吸收率在该波长6上计算浓度。相应地对浓度进行缩放, 以解释库存溶液的稀释。 注: 对于典型的合成, TPs 的收率为 ~ 80%, 20% 是 bipods 和三脚架。</li>
	</ol>
</li>
</ol>6. 小平面活化和纳米结构的连接
<ol><li>
准备胺 (多哈回合) 股票解决方案。
	<ol>
<li>通过在5毫升的乙醇中加入0.140 克的多哈回合来准备一个股票解决方案。几种的解决方案在37赫和 320 W 为〜5分钟, 以确保多哈裁军部完全解散。</li>
	</ol>
</li>
	<li>
阳离子交换和连接。
	<ol>
<li>在适当浓度下准备1毫升的 NC (NR 或 TP) 解决方案 (请参见表 1)。添加6毫克的 ODPA 到1毫升的 NC 溶液和几种10分钟, 在37赫和 320 W。</li>
		<li>另外, 在适当的浓度 (参见表 1) 中, 在一个小瓶中混合1毫升的裁军部股票解决方案和1毫升 Ag+解决方案。加入一个磁性搅拌棒, 并积极搅拌解决方案在 800 RPM。</li>
		<li>在搅拌时, 将 NC 解决方案的1毫升添加到小瓶中, 并允许响应在相应的时间内进行, 如表 1所示。</li>
		<li>在反应结束时, 停止搅拌, 让溶液分离。提取和除去底水层。将5毫升甲醇添加到有机层中, 使其沉淀出来. 离心瓶3分钟在 2240 x g。</li>
		<li>离心后, 丢弃上清液, 加入1毫升的甲苯, re-disperse 该产品以作进一步的表征。 注: 不同材料的阳离子交换反应发生率不同。表 1总结了合成其他材料和形貌的连接纳米结构的条件集。如前所述, 在每组反应中, NC 溶液 (1 毫升)、水性 Ag+溶液 (1 毫升) 和乙醇-多哈解决方案 (1 毫升) 和 ODPA (6 毫克) 的数量保持不变。</li>
	</ol>
</li>
</ol>

<ol>
	<li>Figuerola, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=End-to-End+Assembly+of+Shape-Controlled+Nanocrystals+via+a+Nanowelding+Approach+Mediated+by+Gold+Domains.">End-to-End Assembly of Shape-Controlled Nanocrystals via a Nanowelding Approach Mediated by Gold Domains.</a> Adv. Mat. 21, 550-554 (2009).</li><li>Tang, Z., Kotov, N. A., Giersig, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spontaneous+Organization+of+Single+CdTe+Nanoparticles+into+Luminescent+Nanowires.">Spontaneous Organization of Single CdTe Nanoparticles into Luminescent Nanowires.</a> Science. 297, 237-240 (2002).</li><li>Kim, D., Kim, W. D., Kang, M. S., Kim, S. -. H., Lee, D. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-Organization+of+Nanorods+into+Ultra-Long+Range+Two-Dimensional+Monolayer+End-to-End+Network.">Self-Organization of Nanorods into Ultra-Long Range Two-Dimensional Monolayer End-to-End Network.</a> Nano Lett. 15, 714-720 (2015).</li><li>Schliehe, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrathin+PbS+Sheets+by+Two-Dimensional+Oriented+Attachment.">Ultrathin PbS Sheets by Two-Dimensional Oriented Attachment.</a> Science. 329, 550-553 (2010).</li><li>Chakrabortty, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facet+to+facet+Linking+of+Shape+Anisotropic+Inorganic+Nanocrystals+with+Site+Specific+and+Stoichiometric+Control.">Facet to facet Linking of Shape Anisotropic Inorganic Nanocrystals with Site Specific and Stoichiometric Control.</a> Nano Lett. 16, 6431-6436 (2016).</li><li>Leatherdale, C. A., Woo, W. K., Mikulec, F. V., Bawendi, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+Absorption+Cross+Section+of+CdSe+Nanocrystal+Quantum+Dots.">On the Absorption Cross Section of CdSe Nanocrystal Quantum Dots.</a> J Phys Chem B. 106, 7619-7622 (2002).</li><li>Dabbousi, B. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=(CdSe)ZnS+Core-Shell+Quantum+Dots:+Synthesis+and+Characterization+of+a+Size+Series+of+Highly+Luminescent+Nanocrystallites.">(CdSe)ZnS Core-Shell Quantum Dots: Synthesis and Characterization of a Size Series of Highly Luminescent Nanocrystallites.</a> J Phys Chem B. 101, 9463-9475 (1997).</li><li>Carbone, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis+and+Micrometer-Scale+Assembly+of+Coilloidal+CdSe/CdS+Nanorods+Prepared+by+Seeded+Grwoth+Approach.">Synthesis and Micrometer-Scale Assembly of Coilloidal CdSe/CdS Nanorods Prepared by Seeded Grwoth Approach.</a> Nano Lett. 7, 2942-2950 (2007).</li><li>Shaviv, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Absorption+properties+of+Metal-Semiconductor+Hybrid+Nanoparticles.">Absorption properties of Metal-Semiconductor Hybrid Nanoparticles.</a> ACS Nano. 5, 4712-4719 (2011).</li><li>Lim, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Controlled+Synthesis+of+CdSe+Tetrapods+with+High+Morphological+Uniformity+by+the+Persistent+Kinetic+Growth+and+the+Halide-Mediated+Phase+Transformation.">Controlled Synthesis of CdSe Tetrapods with High Morphological Uniformity by the Persistent Kinetic Growth and the Halide-Mediated Phase Transformation.</a> Chem Mat. 25, 1443-1449 (2013).</li></ol>

我们没有什么要透露的。

这项工作中描述的连接技术允许形状各向异性的镉硫纳米粒子, 可以进行阳离子交换与 Ag+ , 以加入, facet-to-facet, 进入组件, 如线性链或分支网络。未能形成分散, 广泛的 facet-to-facet 连接的纳米粒子的组装往往是因为两个原因: (i) ODPA 在 NR 的溶液中没有很好的分散, 可以通过 sonicating 的混合物来解决。《议定书》详细规定的时间;或者 (ii) 所使用的 Ag+的浓度是非的。当 Ag 的+?...

胶体半导体的定向组装为纳米结构的制备提供了一条合成途径, 其物理化学性质是与各自的 NC 构造块的集合总和或根本不同1,2,3,4. 在纳米粒子组装的各种方法中, 定向 attachment-in 的方法在本质上是相互融合的, 它是一种允许间电子耦合的方式。然而, 传统的定向附着通常需要微妙的平衡粒子偶极子-, 配体-和溶剂的相互作用通常难以执行, 并使适用于不同的 NC 系统。
我们最近开发了一个湿化学方法的共价连接形状-各向异性镉硫, 通过引入一个活性无机中间体, 通过选址的核过程。微粒由反应的无机中间领域的自发融合以后连接5。尽管该技术仍然基于面向对象的连接机制, 但需要考虑弱的间交互, 从而使其具有更大的灵活性和控制能力。通过部分阳离子交换过程 (溶液中) 先将其尖端面转换为银硫, 以实现形状各向异性镉硫的连接;其次是选择性去除的配体钝化表面。然后, 通过融合暴露的银硫面, 形成了与之相连的镉硫的组装。
在本协议中, 我们证明了连接技术可以应用于各种形状的各向异性镉硫 (即CdSe 种子 CdS NRs 和 CdSe 种子 CdSe NRs 或 TPs), 产生长线性 NR 链或高分枝 TP网络.这些结果表明, 该技术可以推广到各种 NC 形状和金属硫, 适于银阳离子交换。

<table width="724">
	<colgroup>
		<col />
		<col span="2" />
		<col />
	</colgroup>
	<tbody>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Cadmium oxide (CdO), 99.5%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Highly toxic</td>
		</tr>
		<tr height="43">
			<td height="43" style="width:288px;height:43px;">Tri-n-octylphosphine oxide (TOPO), 90 % and 99%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Technical and analytical grade</td>
		</tr>
		<tr height="47">
			<td height="47" style="width:288px;height:47px;">Cadmium acetylacetonate (Cd(acac)2), 99.9%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Highly toxic</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Hexadecanediol (HDDO), 90%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Technical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">1-octadecene (ODE), 90%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Technical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Dodecylamine (DDA), 98%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Toxic</td>
		</tr>
		<tr height="47">
			<td height="47" style="width:288px;height:47px;">Cadmium nitrate tetrahydrate ((CdNO3)2.4H2O), 98%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Highly toxic</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Myristic acid (MA), 99%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Octyl phosphonic acid (OPA), 97%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Oleylamine (Oly), 70%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Technical grade</td>
		</tr>
		<tr height="43">
			<td height="43" style="width:288px;height:43px;">Hexadecyltrimethylammonium bromide (CTAB), 95%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Toxic</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Selenium pellets (Se, 5 mm), 99.99%</td>
			<td style="width:123px;">Sigma Aldrich</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Hexadecylamine (HDA), 90%</td>
			<td style="width:123px;">Alfa Aesar</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Technical grade, toxic</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">n-tetradecylphosphonic acid (TDPA), 98%&#160;</td>
			<td style="width:123px;">Alfa Aesar</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade</td>
		</tr>
		<tr height="26">
			<td height="26" style="width:288px;height:26px;">Silver nitrate (AgNO3), 99.9%</td>
			<td style="width:123px;">Alfa Aesar</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Oleic acid (OA), 90%</td>
			<td style="width:123px;">Alfa Aesar</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Technical grade</td>
		</tr>
		<tr height="43">
			<td height="43" style="width:288px;height:43px;">Tri-n-octylphosphine (TOP), 97%</td>
			<td style="width:123px;">Strem</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade, toxic, air sensitive</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">n-hexylphosphonic acid (HPA), 97%</td>
			<td style="width:123px;">Strem</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">n-octadecylphosphonic acid (ODPA), 97%</td>
			<td style="width:123px;">Strem</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Tellurium powder (Te), 99.9%</td>
			<td style="width:123px;">Strem</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Air sensitive</td>
		</tr>
		<tr height="43">
			<td height="43" style="width:288px;height:43px;">Tri-n-butylphosphine (TBP), 99%</td>
			<td style="width:123px;">Strem</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Analytical grade, highly toxic, air sensitive</td>
		</tr>
		<tr height="22">
			<td height="22" style="width:288px;height:22px;">Diisooctylphosphonic acid (DIPA), 90%</td>
			<td style="width:123px;">Fluka</td>
			<td style="width:123px;"></td>
			<td style="width:191px;">Technical grade, toxic</td>
		</tr>
	</tbody>
</table>

facet-to-facet linking of shape-anisotropic colloidal cadmium chalcogenide nanostructures

在这里, 我们描述了一个协议, 允许形状-各向异性的镉硫纳米晶 (如纳米棒 (NRs) 和四 (TPs), 以共价键和现场-具体连接通过其端面, 导致聚合物样的线性或分支链.连接过程以阳离子交换过程开始, 其中镉硫的端面首先转换为银硫。其次是选择性地去除其表面的配体。这就导致了镉硫的高活性银硫端面, 自发地融合在一起, 从而建立了间 facet-to-facet 附件。通过明智地选择前体浓度, 可以产生一个广泛的联网网络。通过低分辨率透射电子显微镜 (TEM) 和能量色散 X 射线光谱学进行了结构表征, 证实了银硫域之间的链镉硫。

使用 CdSe 的 cd NRs 作为模型系统, 如图 1(a)中所示, 我们演示了可以使用部分 Ag+交换过程来专门转换 NR 提示到 ag2的小平面。Ag2的小平面是由多哈回合, 它与 ODPA 反应通过酸碱反应形成一个不溶性盐5。这将导致从 Ag2的小平面中移除 "裁军部" 配体, 从而使它们在接触时相互融合, 并形成链接?...

Watch this Scientific Journal Video about Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures at JoVE.com

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures

本协议详细介绍了形状-各向异性胶体镉硫纳米晶如何通过它们的端面进行共价键连接。

形状-各向异性胶体镉硫纳米结构的 Facet-to-facet 连接

Here, we describe a protocol that allows for shape-anisotropic cadmium chalcogenide nanocrystals (NCs), such as nanorods (NRs) and tetrapods (TPs), to be ...

facet-to-facet-linking-shape-anisotropic-colloidal-cadmium

Research

JoVE Journal

Chemistry

7.6K 次观看.  National University of Singapore. 本协议详细介绍了形状-各向异性胶体镉硫纳米晶如何通过它们的端面进行共价键连接。

视频: Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures

Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules

Nanoporous metal foams possess a unique combination of properties - they are catalytically active, thermally and electrically conductive, and furthermore, have high porosity, high surface-to-volume and strength-to-weight ratio. Unfortunately, common approaches for preparation of metallic nanostructures render materials with highly disordered architecture, which might have an adverse effect on their mechanical properties. Block copolymers have the ability to self-assemble into ordered nanostructures and can be applied as templates for the preparation of well-ordered metal nanofoams. Here we describe the application of a block copolymer-based supramolecular complex - polystyrene-block-poly(4-vinylpyridine)(pentadecylphenol) PS-b-P4VP(PDP) - as a precursor for well-ordered nickel nanofoam. The supramolecular complexes exhibit a phase behavior similar to conventional block copolymers and can self-assemble into the bicontinuous gyroid morphology with two PS networks placed in a P4VP(PDP) matrix. PDP can be dissolved in ethanol leading to the formation of a porous structure that can be backfilled with metal. Using electroless plating technique, nickel can be inserted into the template&#39;s channels. Finally, the remaining polymer can be removed via pyrolysis from the polymer/inorganic nanohybrid resulting in nanoporous nickel foam with inverse gyroid morphology.

This article describes the preparation of well-ordered nickel nanofoams via electroless metal deposition onto nanoporous templates obtained from self-assembled diblock copolymer based supramolecules.

Gyroid镍纳米结构的嵌段共聚物超分子

Nanoporous metal foams possess a unique combination of properties - they are catalytically active, thermally and electrically conductive, and furthermore, ...

科研

化学

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy

Colloidal Probe Nanoscopy (CPN), the study of the nano-scale interactive forces between a specifically prepared colloidal probe and any chosen substrate using the Atomic Force Microscope (AFM), can provide key insights into physical interactions present within colloidal systems. Colloidal systems are widely existent in several applications including, pharmaceuticals, foods, paints, paper, soil and minerals, detergents, printing and much more.1-3 Furthermore, colloids can exist in many states such as emulsions, foams and suspensions. Using colloidal probe nanoscopy one can obtain key information on the adhesive properties, binding energies and even gain insight into the physical stability and coagulation kinetics of the colloids present within. Additionally, colloidal probe nanoscopy can be used with biological cells to aid in drug discovery and formulation development. In this paper we describe a method for conducting colloidal probe nanoscopy, discuss key factors that are important to consider during the measurement, and show that both quantitative and qualitative data that can be obtained from such measurements.

Colloidal probe nanoscopy can be used within a variety of fields to gain insight into the physical stability and coagulation kinetics of colloidal systems and aid in drug discovery and formulation sciences using biological systems. The method described within provides a quantitative and qualitative means to study such systems.

粒子粒子相互作用采用胶体探针纳米显微定量和定性检查

Colloidal Probe Nanoscopy (CPN), the study of the nano-scale interactive forces between a specifically prepared colloidal probe and any chosen substrate ...

Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Indoxyl glycosides proved to be valuable and versatile tools for monitoring glycosidase activities. Indoxyls are released by enzymatic hydrolysis and are rapidly oxidized, for example by atmospheric oxygen, to indigo type dyes. This reaction enables fast and easy screening in vivo without isolation or purification of enzymes, as well as rapid tests on agar plates or in solution (e.g., blue-white screening, micro-wells) and is used in biochemistry, histochemistry, bacteriology and molecular biology. Unfortunately the synthesis of such substrates proved to be difficult, due to various side reactions and the low reactivity of the indoxyl hydroxyl function. Especially for glucose type structures low yields were observed. Our novel approach employs indoxylic acid ester as key intermediates. Indoxylic acid esters with varied substitution patterns were prepared on scalable pathways. Phase transfer glycosylations with those acceptors and peracetylated glycosyl halides can be performed under common conditions in high yields. Ester cleavage and subsequent mild silver mediated glycosylation yields the peracetylated indoxyl glycosides in high yields. Finally deprotection is performed according to Zempl&#233;n.

Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme activity monitoring. Especially for glucose type structures previous syntheses proved to be challenging and low yielding. Our novel approach employs indoxylic acid esters as precious intermediates to yield a considerable number of indoxyl glycosides in good yields.

的吲哚酚苷的检测糖苷酶活动的合成

Indoxyl glycosides proved to be valuable and versatile tools for monitoring glycosidase activities. Indoxyls are released by enzymatic hydrolysis and are ...

Synthesis and Exfoliation of Discotic Zirconium Phosphates to Obtain Colloidal Liquid Crystals

Due to their abundance in natural clay and potential applications in advanced materials, discotic nanoparticles are of interest to scientists and engineers. Growth of such anisotropic nanocrystals through a simple chemical method is a challenging task. In this study, we fabricate discotic nanodisks of zirconium phosphate [Zr(HPO4)2&#183;H2O] as a model material using hydrothermal, reflux and microwave-assisted methods. Growth of crystals is controlled by duration time, temperature, and concentration of reacting species. The novelty of the adopted methods is that discotic crystals of size ranging from hundred nanometers to few micrometers can be obtained while keeping the polydispersity well within control. The layered discotic crystals are converted to monolayers by exfoliation with tetra-(n)-butyl ammonium hydroxide [(C4H9)4NOH, TBAOH]. Exfoliated disks show isotropic and nematic liquid crystal phases. Size and polydispersity of disk suspensions is highly important in deciding their phase behavior.

A two dimensional model material of discotic zirconium phosphate was developed. The inorganic crystal with lamellar structure was synthesized by hydrothermal, reflux, and microwave-assisted methods. On exfoliation with organic molecules, layered crystals can be converted to monolayers, and nematic liquid crystal phase was formed at sufficient concentration of monolayers.

合成与盘状磷酸盐锆剥落获取胶体液晶

Due to their abundance in natural clay and potential applications in advanced materials, discotic nanoparticles are of interest to scientists and ...

Visualization of Ambient Mass Spectrometry with the Use of Schlieren Photography

This manuscript outlines how to visualize mass spectrometry ambient ionization sources using schlieren photography. In order to properly optimize the mass spectrometer, it is necessary to characterize and understand the physical principles of the source. Most commercial ambient ionization sources utilize jets of nitrogen, helium, or atmospheric air to facilitate the ionization of the analyte. As a consequence, schlieren photography can be used to visualize the gas streams by exploiting the differences in refractive index between the streams and ambient air for visualization in real time. The basic setup requires a camera, mirror, flashlight, and razor blade. When properly configured, a real time image of the source is observed by watching its reflection. This allows for insight into the mechanism of action in the source, and pathways to its optimization can be elucidated. Light is shed on an otherwise invisible situation.

This paper presents a protocol for the visualization of gaseous streams of an ambient ionization source using schlieren photography and mass spectrometry.

环境质谱可视化纹影摄影的使用

This manuscript outlines how to visualize mass spectrometry ambient ionization sources using schlieren photography. In order to properly optimize the mass ...

Preparation and Characterization of C60/Graphene Hybrid Nanostructures

Physical thermal deposition in a high vacuum environment is a clean and controllable method for fabricating novel molecular nanostructures on graphene. We present methods for depositing and passively manipulating C60 molecules on rippled graphene that advance the pursuit of realizing applications involving 1D C60/graphene hybrid structures. The techniques applied in this exposition are geared towards high vacuum systems with preparation areas capable of supporting molecular deposition as well as thermal annealing of the samples. We focus on C60 deposition at low pressure using a homemade Knudsen cell connected to a scanning tunneling microscopy (STM) system. The number of molecules deposited is regulated by controlling the temperature of the Knudsen cell and the deposition time. One-dimensional (1D) C60 chain structures with widths of two to three molecules can be prepared via tuning of the experimental conditions. The surface mobility of the C60 molecules increases with annealing temperature allowing them to move within the periodic potential of the rippled graphene. Using this mechanism, it is possible to control the transition of 1D C60 chain structures to a hexagonal close packed quasi-1D stripe structure.

Preparation and Characterization of C60/Graphene Hybrid Nanostructures

Here we present a protocol for the fabrication of C60/graphene hybrid nanostructures by physical thermal evaporation. Particularly, the proper manipulation of deposition and annealing conditions allow the control over the creation of 1D and quasi 1D C60 structures on rippled graphene.

C 型60/石墨烯混合纳米结构的制备与表征

Physical thermal deposition in a high vacuum environment is a clean and controllable method for fabricating novel molecular nanostructures on graphene. We ...

The Effect of Ultraviolet Radiation on the Chemical Bath Deposition of Bis(thiourea) Cadmium Chloride Crystals and the Subsequent CdS Obtention

In this work, the effects on the preparation of bis(thiourea) cadmium chloride crystals when illuminated with ultraviolet (UV) light at a wavelength of 367 nm using the chemical bath deposition technique are studied comparatively. Two experiments are performed to make a comparison: one without UV light and the other with the aid of UV light. Both experiments are performed under equal conditions, at a temperature of 343 K and with a pH of 3.2. The precursors used are cadmium chloride (CdCl2) and thiourea [CS(NH2)2], which are dissolved in 50 mL of deionized water with an acidic pH. In this experiment, the interaction of electromagnetic radiation is sought at the moment the chemical reaction is carried out. The results demonstrate the existence of an interaction between the crystals and the UV light; the UV light assistance causes crystal growths in an acicular shape. Also, the final product obtained is cadmium sulfide and shows no evident difference when synthesized with or without the use of UV light.

The Effect of Ultraviolet Radiation on the Chemical Bath Deposition of Bisthiourea Cadmium Chloride Crystals and the Subsequent CdS Obtention

This article presents a protocol for the synthesis of bis(thiourea) cadmium chloride crystals by chemical bath deposition. Two experiments are described: one aided by ultraviolet light compared to one without ultraviolet light.

紫外辐射对双 (硫脲) 氯化镉晶体化学镀液沉积的影响及随后的 CdS 获得

In this work, the effects on the preparation of bis(thiourea) cadmium chloride crystals when illuminated with ultraviolet (UV) light at a wavelength of ...

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F&#8722;

Anion photoelectron imaging is a very efficient method for the study of energy states of bound negative ions, neutral species and interactions of unbound electrons with neutral molecules/atoms. State-of-the-art in vacuo anion generation techniques allow application to a broad range of atomic, molecular, and cluster anion systems. These are separated and selected using time-of-flight mass spectrometry. Electrons are removed by linearly polarized photons (photo detachment) using table-top laser sources which provide ready access to excitation energies from the infra-red to the near ultraviolet. Detecting the photoelectrons with a velocity mapped imaging lens and position sensitive detector means that, in principle, every photoelectron reaches the detector and the detection efficiency is uniform for all kinetic energies. Photoelectron spectra extracted from the images via mathematical reconstruction using an inverse Abel transformation reveal details of the anion internal energy state distribution and the resultant neutral energy states. At low electron kinetic energy, typical resolution is sufficient to reveal energy level differences on the order of a few millielectron-volts, i.e., different vibrational levels for molecular species or spin-orbit splitting in atoms. Photoelectron angular distributions extracted from the inverse Abel transformation represent the signatures of the bound electron orbital, allowing more detailed probing of electronic structure. The spectra and angular distributions also encode details of the interactions between the outgoing electron and the residual neutral species subsequent to excitation. The technique is illustrated by the application to an atomic anion (F&#8722;), but it can also be applied to the measurement of molecular anion spectroscopy, the study of low lying anion resonances (as an alternative to scattering experiments) and femtosecond (fs) time resolved studies of the dynamic evolution of anions.

Here, we present a protocol for photoelectron imaging of anionic species. Anions generated in vacuo and separated by mass spectrometry are probed using velocity mapped photoelectron imaging, providing details of anion and neutral energy levels, anion and neutral structure and the nature of the anion electronic state.

由 F− 310 Nm 支队所示的阴离子光电子成像

Anion photoelectron imaging is a very efficient method for the study of energy states of bound negative ions, neutral species and interactions of unbound ...

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

This article presents a detailed protocol for synthesis of small circular DNA molecules, annealing of circular DNA motifs, and construction of 1D and 2D DNA nanostructures. Over decades, the rapid development of DNA nanotechnology is attributed to the use of linear DNAs as the source materials. For example, the DAO (double crossover, antiparallel, odd half-turns) tile is well-known as a building block for construction of 2D DNA lattices; the core structure of DAO is made from two linear single-stranded (ss) oligonucleotides, like two ropes making a right hand granny&#160;knot. Herein, a new type of DNA tiles called cDAO (coupled DAO) are built using a small circular ss-DNA of c64nt or c84nt (circular 64 or 84 nucleotides) as the scaffold strand and several linear ss-DNAs as the staple strands. Perfect 1D and 2D nanostructures are assembled from cDAO tiles: infinite nanowires, nanospirals, nanotubes, nanoribbons; and finite nano-rectangles. Detailed protocols are described: 1) preparation by T4 ligase and purification by denaturing PAGE (polyacrylamide gel electrophoresis) of small circular oligonucleotides, 2) annealing of stable circular tiles, followed by native PAGE analysis, 3) assembling of infinite 1D nanowires, nanorings, nanospirals, infinite 2D lattices of nanotubes and nanoribbons, and finite 2D nano-rectangles, followed by AFM (Atomic Force Microscopy) imaging. The method is simple, robust, and affordable for most labs.

This article presents a detailed protocol for T4 ligation and denaturing PAGE purification of small circular DNA molecules, annealing and native PAGE analysis of circular tiles, assembling and AFM imaging of 1D and 2D DNA nanostructures, as well as agarose gel electrophoresis and centrifugation purification of finite DNA nanostructures.

由小圆形 DNA 分子构建的稳定 DNA 分子、一维和二维纳米结构

This article presents a detailed protocol for synthesis of small circular DNA molecules, annealing of circular DNA motifs, and construction of 1D and 2D ...

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

DNA origami nanostructures hold an immense potential to be used for biological and medical applications. However, low-salt conditions and nucleases in physiological fluids induce denaturation and degradation of self-assembled DNA nanostructures. In non-viral gene delivery, enzymatic degradation of DNA is overcome by the encapsulation of the negatively charged DNA in a cationic shell. Herein, inspired by gene delivery advancements, a simple, one-step and robust methodology is presented for the stabilization of DNA origami nanostructures by coating them with chitosan and linear polyethyleneimine. The polycation coating efficiently protects DNA origami nanostructures in Mg-depleted and nuclease-rich media. This method also preserves the full addressability of enzyme- and aptamer-based functionalization of DNA nanostructures.

Here, a protocol for the protection of DNA origami nanostructures in Mg-depleted and nuclease-rich media using natural cationic polysaccharide chitosan and synthetic linear polyethyleneimine (LPEI) coatings is presented.

基因治疗诱导聚化涂层保护 dna 折纸纳米结构

DNA origami nanostructures hold an immense potential to be used for biological and medical applications. However, low-salt conditions and nucleases in ...