Prepare Apparatus for Dispensing Addressable Droplets
3:26
Form Addressable Droplets and Load with Single Cells
7:42
Data Analysis
9:39
Results: Single Cell Data
11:08
Conclusion
Transcript
The overall goal of this experiment is to determine the absolute protein copy number of a target protein with single-cell resolution. Single cell techniques will enable a better understanding of the behavior of a protein as it relates to a central
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Here we present addressable droplet microarrays (ADMs), a droplet array based method able to determine absolute protein abundance in single cells. We demonstrate the capability of ADMs to characterize the heterogeneity in expression of the tumor suppressor protein p53 in a human cancer cell line.
