Results: Clear Antibody Staining that Reveals Drosophila Structure and Cellular Organization
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Conclusion
副本
The overall goal of this procedure is to dissect and stain drosophila pupal ovaries. This method can help answer key questions in development of biology, such as those pertaining to stem cell differentiation and tissue development in the model org
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The Drosophila ovary is an excellent model system for studying stem cell niche development. Though methods for dissecting larval and adult ovaries have been published, pupal ovary dissections require different techniques that have not been published in detail. Here we outline a protocol for dissecting, staining, and mounting pupal ovaries.