Chemical Conjugation of HEVNPs with Biotin, Cancer Targeting Ligand, and Fluorophores
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Results: Production of LXY30-HEVNPs and Binding/Internalization of Fluorescently Labeled LXY30-HEVNPs in MDA-MB-231 Breast Cancer Cells
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Conclusion
Transcript
The overall goal of chemical functionalization of the surface of HEV nanoparticles is to provide a repeatable and consistent approach for conjugation of targeting immunogenic, therapeutic and diagnostic molecules to the surface of HEV nanoparticle
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We have engineered the capsid protein of hepatitis E virus as a theranostic nanoparticle (HEVNP). HEVNP self-assembles into a stable icosahedral cage in mucosal delivery. Here, we describe the modification of HEVNPs for tumor targeting by mutating surface-exposed residues to cysteines, which conjugate synthetic ligands that specifically bind tumor cells.
