Batch Processing of ICS Datasets and Calculation of the Microscope s Beam Area
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Results: Image Correlation Spectroscopy Detects Clusters on the Cell Surface of Adherent Cancer Cells
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Conclusion
副本
The overall goal of this image correlation spectroscopy protocol is to provide an accessible methodology for the quantification of clustering events occurring at the cell surface. Antibodies that bind to receptors at the cell surface can confer co
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Antibodies that bind to target receptors on the cell surface can confer conformation and clustering alterations. These dynamic changes have implications for characterizing drug development in target cells. This protocol utilizes confocal microscopy and image correlation spectroscopy through ImageJ/FIJI to quantify the extent of receptor clustering on the cell surface.