Preparation of Primary Cells from Zebrafish Embryos
1:54
Preparation of Reusable Poly-L-lysine-coated Glass Bottom Dishes
3:26
Preparation and Plating of Primary Cells
6:02
Transfection of Primary Cells with Plasmid DNA
6:57
Results: Live Cell Imaging of Zebrafish Primary Cells
8:25
Conclusion
副本
This method provides the zebrafish community a tool for the the analysis of intercellular dynamics with a resolution unattainable in vivo. And also it allows to compensate for the lack of zebrafish cell lines. The main advantage of this technique
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We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.
