July 26th, 2018
•Ribonucleotides are among the most abundant non-canonical nucleotides incorporated into the genome during eukaryotic nuclear DNA replication. If not properly removed, ribonucleotides can cause DNA damage and mutagenesis. Here, we present two experimental approaches that are used to assess the abundance of ribonucleotide incorporation into DNA and its mutagenic effects.
Tags
Related Videos
Measuring Replicative Life Span in the Budding Yeast
Gross and Fine Dissection of Inner Ear Sensory Epithelia in Adult Zebrafish (Danio rerio)
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Competitive Genomic Screens of Barcoded Yeast Libraries
Single Oocyte Bisulfite Mutagenesis
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA