Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
Results: FKBP12 Dimerizes upon Addition of BB Dimerizer Drug
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Conclusion
副本
This method can help answer key questions in the drug screening field, such elucidating the effect of small-molecule inhibitors on very low concentrations for protein-protein interactions. The main advantage of this technique is that it is calibra
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This protocol describes a calibration-free approach for quantifying protein homo-oligomerization in vitro based on fluorescence fluctuation spectroscopy using commercial light scanning microscopy. The correct acquisition settings and analysis methods are shown.