Preparation of the Cells for Transfection and Transfection of the Cells
3:54
Analysis of Cell Transfection Efficacy Using Flow Cytometry and Fluorescence Microscopy
5:33
Results: Analysis of Generated Cationic Nanoliposomes
7:13
Conclusion
副本
This protocol allows the production of safe and efficient nanoliposomes which can be used as transfection vehicles for therapeutic messenger RNA. These nanoliposomes facilitate mRNA uptake in cells resulting in increased protein expression levels
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Here we describe a protocol for the generation of cationic nanoliposomes, which is based on the dry-film method and can be used for the safe and efficient delivery of in vitro transcribed messenger RNA.