Printing Microenvironment Microarrays (MEMAs) Using an Array Printing Robot
1:37
Creation of Ligand Treatment Plates
2:28
Culturing Cells on MEMAs
4:23
Fixing, Staining, and Imaging MEMAs
6:14
Results: Cell Cycle Profiles, Cell Number and EdU Incorporation
7:44
Conclusion
副本
Studying the tumor microenvironment is difficult due to its complexity. We've developed microenvironment microarrays consisting of simple combinatorial microenvironments that allow the identification of major drivers of tumor cell phenotypes. The
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The purpose of the method presented here is to show how microenvironment microarrays (MEMA) can be fabricated and used to interrogate the impact of thousands of simple combinatorial microenvironments on the phenotype of cultured cells.