September 5th, 2019
•Here, we present a protocol to dissociate and sort a specific cell population from the Drosophila male accessory glands (secondary cells) for RNA sequencing and RT-qPCR. Cell isolation is accomplished through FACS purification of GFP-expressing secondary cells after a multistep-dissociation process requiring dissection, proteases digestion and mechanical dispersion.
Related Videos
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
The TreadWheel: Interval Training Protocol for Gently Induced Exercise in Drosophila melanogaster
Purification of Low-abundant Cells in the Drosophila Visual System
Generation of Cancer Cell Clones to Visualize Telomeric Repeat-containing RNA TERRA Expressed from a Single Telomere in Living Cells
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
Use of Drosophila S2 Cells for Live Imaging of Cell Division