Results: Representative Secondary Cell (SC) RNA Isolation and Analysis
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Conclusion
Transcription
This method permits a transcriptomic analysis of a rare cell population from Drosophila male accessory gland, called the secondary cells, and it uncovers genes that make them critical for reproductive success. In the course of one day, this techni
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Here, we present a protocol to dissociate and sort a specific cell population from the Drosophila male accessory glands (secondary cells) for RNA sequencing and RT-qPCR. Cell isolation is accomplished through FACS purification of GFP-expressing secondary cells after a multistep-dissociation process requiring dissection, proteases digestion and mechanical dispersion.