Optional Surgical Trachea and Carotid Artery Preparation
3:06
Surgical Cremaster Muscle Preparation
4:44
Intravital Microscopy
5:38
Results: Representative Post-Capillary Venule Identification, Rolling Leukocyte Recruitment Patterns, and Hemodynamic and Microvascular Parameter Assessment
7:21
Conclusion
副本
This practical and reproducible method can be used to visualize the endothelium leukocyte interactions that lead to leukocyte recruitment within an intact mouse. This technique also presents a powerful tool for the monitoring of physiological and
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Here, we show how to perform intravital microscopy on post-capillary venules of the mouse cremaster muscle. Commonly applied to different models of inflammation and sepsis, particularly those induced by chemokines and cytokines, we highlight its relevance in the study of muscolopathies involving exaggerated muscular leukocyte infiltration.
