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14:41 min
July 11th, 2020
DOI :
10.3791/61394-v
Chapters
0:00
Introduction
1:27
Step 1: Preparation of a 15% Denaturing Polyacrylamide Gel
2:03
Step 2: Assembly of the Glass Plates and Electrophresis Unit
4:13
Step 3: Preparation of Loading Dye and Samples
5:38
Step 4: Gel Electrophresis
6:33
Step 5: Preparation for Electro-blotting
10:15
Step 6: Preparation of Radiolabeled Probe
10:50
Step 7: Hybridization of the Blot
12:36
Representative Results
13:24
Conclusion
Transcript
Hi there. So this is a small RNA northern protocol. It's a derivative of protocol originally optimized by Dr.Rashid Akbergenov.
In this method, one can detect small RNA's of anywhere between 20-24 nucleotide in length, very precisely and at
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Summary
This method demonstrates use of the northern hybridization technique to detect miRNAs from total RNA extract.
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