November 30th, 2021
•This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.
Related Videos
Human ES cells: Starting Culture from Frozen Cells
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
In Vivo Modeling of the Morbid Human Genome using Danio rerio
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
An Ex vivo Culture System to Study Thyroid Development
Establishment of an Extracellular Acidic pH Culture System
A High Output Method to Isolate Cerebral Pericytes from Mouse
Isolation of Human Primary Valve Cells for In vitro Disease Modeling
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint